查询词典 chemiluminescent

The invention relates to a method for adopting acridine ester DMAE.NHS as chemiluminescent substance for analyzing the total thyroxine.

本发明涉及一种采用吖啶酯DMAENHS作为化学发光物质分析总甲状腺素的方法。

Methods Highly purified HCV Core, NS3, NS4, NS5 proteins were immobilized on solid matrix by biochip arrayer, the protein chip was used to detect the corresponding antibodies in human serum by chemiluminescent immunoassays.

利用生物芯片技术和化学发光技术将高度纯化的抗原HCV Core、NS3、NS4、NS5以特定微阵列固定在固相载体上，用化学发光免疫法检测抗HCV Core、抗HCV NS3、抗HCV NS4和抗HCV NS5。

A flow injection method was established for the determination of gallic acid in gallnut with enhanced chemiluminescent detection based on Luminol-KIO4 system.

在碱性条件下，高碘酸钾氧化鲁米诺可产生化学发光，没食子酸对其有很强的增敏作用。

We detected fasting plasma glucose by glucose oxidase,fasting insulin by chemiluminescent immunoassay, triglyceride and high density lipoprotein-cholesterol by zymology,tumor necrosis factor-αby enzyme linked immunosorbent assay, the reverse of the product of FPG and FIns is used as the insulin sensitivity index,that is,ISI=1/FPG×FIns.

血糖测定采用葡萄糖氧化酶法；胰岛素测定采用化学发光法；血脂测定采用酶法；肿瘤坏死因子-α测定采用双抗体夹心酶联免疫法；胰岛素敏感指数采用空腹血糖与空腹胰岛素乘积的倒数，即ISI=1/FPG ×FIns。

We detected fasting plasma glucose by glucose oxidase, fasting insulin by chemiluminescent immunoassay, triglyceride and high density lipoprotein-cholesterol by zymology, tumor necrosis factor-αby enzyme linked immunosorbent assay, the reverse of the product of FPG and FIns is used as the insulin sensitivity index, that is, ISI=1/FPG×Fins.

血糖测定采用葡萄糖氧化酶法；胰岛素测定采用化学发光法；血脂（甘油三酯TG、高密度脂蛋白HDL-C）测定采用酶法；肿瘤坏死因子-α测定采用双抗体夹心酶联免疫法；胰岛素敏感指数采用空腹血糖与空腹胰岛素乘积的倒数，即ISI=1/FPG ×FIns。

CLIA using acridinium ester derivatives as chemiluminescent label has advantages of low background,high signal-to-noise ratio,no need of catalyst and simple luminescence system.

以吖啶酯衍生物为发光标记物的CLIA的特点是：标记简单、标记物稳定、不需要催化剂、自然本底低、受外界干扰因素少、信噪比高、发光体系简单等。

Different aspects associating the DMAE-NHS-based CLIA were studied in this thesis,including synthesis of acridinium ester,chemiluminescent characteristics of DMAE-NHS,labelling antibody or streptavidin with DMAE-NHS,two-site sandwich chemiluminescent immunoassay for TSH,two-site sandwich chemiluminescent immunoassay involved biotin-streptavidin system for TSH,and competitive chemiluminescent immunoassay using biotin-streptavidin system for TLVThe desired acridinium ester,DMAE-NHS,was synthesized according to the reference method with some modifications.

目前国内尚未见有关DMAE·NHS的合成及其应用于CLIA的研究报导，本论文研究了吖啶酯DMAE·NHS的有机合成方法，DMAE·NHS的发光性能，DMAE·NHS及其与抗体偶联物的热稳定性，DMAE·NHS标记TSH单克隆抗体和链亲和素，TSH CLIA，TSH BAS-CLIA和TT_4 BAS-CLIA。

Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-二甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿／乙酸乙酯(4∶1，V∶V)作溶剂和淋洗剂，第二次柱层析用己烷／丙酮(2∶1，V∶V)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷／丙酮(2∶1，V∶V)进行研磨，过滤，洗涤，除去溶于己烷／丙酮(2∶1，V∶V)的部分，得到所需要的产品。

The chemiluminescent immunoass was used to measure the level of HCG in 49 hydatidiform moles.

采用化学发光法测定了49例葡萄胎患者的308次血人绒毛膜促性腺激素。

In this article, the current and emerging applications of chemiluminescent techniques in the research of life sciences are reviewed, with emphasis on the chemiluminescent detection of nucleic acids and proteins.

自上个世纪七十年代末八十年代初起，化学发光分析法在生命科学研究中的应用逐渐为人们所关注，开始了它发展的第二阶段。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。