查询词典 chelating

The lyophilization treatment of silkie fowl blood meal and the hot-air drying treatment of broiler blood meal had the strongest inhibition in lipid peroxidation. All the blood meal showed higher ability of the ferrous ion chelating.

而所有的血粉样品其螯合亚铁离子能力都达到90%以上，彼此间无显著差异，血粉样品的抑制脂质过氧化能力以乌骨鸡冷冻乾燥的血粉效果最佳(p.05)且与自肉鸡热风乾燥之血粉无显著差异。

The scFv formed inclusion body in E. Coli. 8 mol/L urea was used to solubilize the inclusion body, and the scFv was then purified in two steps , including Ni 2+ chelating affinity chromatography and gel filtration.

大肠杆菌表达的单链抗体包涵体，用8 mol/L尿素裂解，经过Ni离子螯合亲和层析和凝胶过滤两步纯化后，纯度达到97%以上，再通过透析复性除去尿素。

A new chelating fiber containing thioamide group is synthesized by cross-linking of polyacrylonitrile fiber with ethylene-dimine and following with chemical modification with sodium sulfide using DMF as solvent.

以腈纶纤维为起始原料，经乙二胺预交联后，以DMF作溶剂与硫化钠反应，合成了一种硫代酰胺功能基可达6.8mmol/g干纤维的螯合功能纤维，其对贵金属金、银离子具有良好的吸附性能。

According to the Mannich-Reaction, a kind of chelating flocculant grafted with diethylene triamine was prepared from polyacrylamide, formaldehyde and diethylene triarmne.

研究了以聚丙烯酰胺、甲醛、二乙烯三胺为原料，按照Mannich反应机理制备部分胺化接枝型螯合作用絮凝剂及其从水中除铅性能。

The results are following:(1)We investigated the influence of the positional isomeric effect on structures.Due to the less space-crowding,it is easier for 3-pytpy to behave as bridging ligand than 2-pytpy under the same reaction conditions,while,it is easier for 2-pytpy to function as tridentate chelating ligand to construct supramolecular structures.

研究结果如下：(1)研究了两个吡啶取代三联吡啶配体的位置异构效应对配合物结构的影响。3-pytpy配体比2-pytpy具有更小的空间位阻，在相同的合成条件下容易以单-三齿桥联方式配位；而2-pytpy更易以三齿螯合形式配位，通过π…π堆积作用形成超分子结构。

In this sense, IDA plays dual effects, tridentate chelating to Cu(2) and bridgebetween two Cu(1) andCu(2).3 Metal complexes were selected as the appropriate for the study of cleavage plasmid pBR322DNA by gel electrophoresis technique. The results showed Ni and Mn complexes could cleave effect- ively DNA in the presence of H2O2 at physiological pH and temperature, whereas individual Zn complex could cleave effectively DNA.

通过电泳实验研究了一系列金属配合物与pBR322DNA的作用，发现在Tris-HCl缓冲溶液中，生理条件下，镍、锰配合物在共反应物H_2O_2存在下能够很好的断裂DNA，而Zn配合物单独作用就能够使DNA由ccc型转化为oc和linear型。

Secondary electroless copper plating system of triethanolamine and EDTA·2Na dual-chelating-agent is researched in the paper.

系统研究了以三乙醇胺为主络合剂、EDTA·2Na盐为辅络合剂的二次镀铜体系。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

In result of DPPH free radical clean test, we got good effect that using 50% ethanol and ethanol extracted Vitaceae plants in the test of ferrous ion chelating effects have over 50% restraint rate by using 50% ethanol.

在清除DPPH自由基方面结果显示，用乙醇及50%乙醇萃取的葡萄科植物具有不错效用，其中50%乙醇萃取细本山葡萄在还原能力测定及螯合亚铁离子实验中皆达到50%以上的抑制率。

In this experiment, we use different kind of solvent to extract Vitaceae plants to test of antioxidae, includes clean DPPH free radical, ferrous ions chelating effects, restrain producing superoxide anion, ferric reducing ability of plasma and anti-liver lipid peroxidation assay.

在利用不同溶媒萃取葡萄科植物评估抗乳癌细胞株MCF-7毒杀试验和抗氧化试验中，其包含清除DPPH自由基、螯合亚铁离子、抑制超氧阴离子之生成、还原三价铁离子能力测定及抗肝脂质过氧化等几项。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。