查询词典 chaperons

Lon protease and members of Clp family of molecular chaperons and protease regulatory subunits contain homologous regions with properties expected for substrate-binding domain.

最近有相关的研究指出，位於大肠杆菌中的 Lon C 端的P domain，主要是负责蛋白酶间的聚合，而辨识基质的部份则位於N端。

Organisms often synthesize molecular chaperons to cope with stress such as heat, cold, wounding, heavy metals, and water deficit.

生物常合成分子保护子来应对高温、低温、伤害、重金属以及缺水等逆境。

With the assistance of the chaperons such as calnexin、calreticulin and tapasin,the molecular heavy chain andβ2 micro globulin of the major histocompatibility complexare assembled together to form the steady complex which is further transported to the surface of the APC through the Golgi body.

在calnexin、calreticulin、tapasin等分子伴侣的协助下，内质网腔内的主要相容性复合体(major histocompatibilitycomplex，MHC)Ⅰ类分子重链、β2微球蛋白以及相应多肽片段聚集成较为稳定的复合物，经高尔基体被进一步转运到APC表面；MHCⅠ类分子与抗原肽的有效结合是特异性细胞免疫应答的重要的环节。

ER stress induces cardiomyocytesapoptosis by activation of ER chaperons such as GRP78 and CHOP,PERK-eIF2αpathway,IRE1-XBP1 pathway,caspase-12 and JNKs pathway.

给予β受体阻滞剂和膜稳定剂在体内体外都能有效抑制内质网应激，减少细胞凋亡。

The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction from the genomic DNA ofM.tuberculosis H37RV strain;(2) To clone PCR product of Tb wbbLgene into a cloning vector pMD18-T for sequencing;(3) To subcloneTb wbbL gene to an expression vector pET16b to construct pET16b-Tb wbbL; and to overexpress Tb WbbL protein in E.coli BL21(DE3)under different induction conditions;(4) To establish theco-expression system for expressing chaperons of pKJE7 plasmidand soluble Tb WbbL protein in E.coli BL21(DE3) under differentinduction conditions;(5) To test expressed WbbL protein by SDS-PAGEand Western blot methods.

本论文的目的是：(1)利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因；(2)将 Tb wbbL 基因克隆到pMD18-T 克隆载体中，经 DNA 序列测定证实为正确的基因；(3)将 Tb wbbL 基因亚克隆到 pET16b 表达载体中并通过改变不同的诱导条件在大肠杆菌 BL21(DE3)中表达 Tb WbbL 蛋白质；(4)建立在 BL21(DE3)大肠杆菌中共表达 Tb WbbL 蛋白质与分子伴侣的体系，优化表达条件以高效表达出可溶性 Tb WbbL蛋白质；(5)用 Western blot 方法鉴定所表达的 Tb WbbL 蛋白质为 Tb wbbL 基因产物。

As a sequence, the cell evolved an adaptively coordinated response to limit further accumulation of unfolded proteins by expressing large amount of chaperons. In this pathway, Ite1, an ER transmembrane protein kinase/endoribonuclease, is essential for viability during ER stress.

当内质网腔内未折叠蛋白增多时，通过Irelp胞质面部分的丝氨酸／苏氨酸自磷酸化，诱导UPR特异性转录因子Haclp mRNA剪接成熟。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。