查询词典 chain-driven

Methods The cDNA coding sequencing of enterokinase light chain was amplified by RT-PCR from mouse dodecadactylon mucosa and cloned into pET32a expression plasmid. The recombinant enterokinase light chain was expressed in BL21(DE3) and purified with Ni-affinity chromatography.

采用RT-PCR从C57BL/6J小鼠的十二指肠肠系膜黏膜组织中钓取肠激酶轻链的cDNA，将其克隆入pET32a原核表达载体中，并在大肠杆菌BL21(DE3)中进行表达，然后以镍亲和层析法对表达产物进行纯化。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

From dynamic customer demand, supply chain is composed of supplier and retailer, combining P-control strategy with z-transformation, a dynamic model is given, building model of traditional, EPOS, VMI supply chain respectively.

研究在动态顾客需求下，生产商和零售商所组成的供应链系统，采用P-控制策略和z变换相结合，给出一个动态模型，并分别对传统、EPOS和VMI供应链进行建模，通过仿真，分析和比较顾客需求信息对三种供应链性能影响的效果。

Comparing with database in GenBank, the results showed RBB1 had 91% homology to mRNA for a known ATP synthase CF1 epsilon chain 89% homology to ATPase epsilon subunit in Zea mays and 87% homology to Triticum aestivum ATP synthase CF1 epsilon chain.

对该基因可读框架编码的蛋白进行功能位点和结构功能域的分析，同样证明了该基因为H〓-ATP合酶CF〓亚基蛋白编码基因，其氨基酸序列3-83之间和1-133位氨基酸之间具有两个保守的蛋白结构域ATP-synt-DE-N和AtpC。

Twentieth century, with the rapid development of the world automobile industry, automotive market prospects after a huge, everlast company decided to divest its existing main business focus on energy-saving auto after-market product development and maintenance services to the most advanced research results: Fuel reinforcing agents, saving energy and reducing friction agent, boric acid ester, organic molybdenum, tungsten, boron products, first applied to the oil gas, diesel additives and car care and maintenance in the field, specifically for the automotive service chain to provide OEM service and technical support Europe and the United States as a world-renowned conservation renowned chain Midas, Firth pass, lead-free round and so on and Everlast company has a good working relationship, enjoys a high reputation.

2002年进入中国，安能公司先后为多家汽车生产厂及汽车护理连锁服务机构提供OEM服务和技术支持，如车爵士、美达等中国品牌，在此期间，安能公司努力适应中国市场情况和实际需求，创新了一套适合中国国情的运营模式。2010年，安能公司斥资千万在中国北京中关村科技园区及上海建立了汽车护理品两个专业研发基地，同时注资北京市级油品应用技术研究所，共同打造具有世界先进水平的汽车护理品研发基地。并顺利开工建设在北京顺义的生产基地，安能以其强大的技术优势和科研优势奠定了发展中国市场的坚实基础。

This is a excellent video for tit torture: you will see the master use a lot of clamps put on model's breast and nipple, also use nipple chain on the nipples, then hang few heavy metal cuff on the nipple chain, flogger strike on her breast, Tongtong's hands are tied up with ropes and hang strait up back, she can do nothing except moaning and screaming for mercy.

您可以在本期录像中看到精彩的虐乳镜头：彤彤的双手被高高地吊在天花板上，主人用夹子夹满了她的乳房和乳头，又用几个钢制手铐穿在一起吊在她的乳头铁链子上，令她发出痛苦的呻吟。您还将看到口枷，鞭打，吊刑，铁笼子等刑具在等着美丽的彤彤！

Product categories include: diamond-studded ornaments clasp, chain-shaped chain buckle, zinc alloy decorative buckle, plastic buckle, mountaineering buckle, shoes buckle floriation, package and, corns, hit nails, claw beads, paint clasp, diamond, acrylic, glass diamond.

产品类别有：镶钻饰扣、链形链扣、锌合金饰扣、塑胶扣、登山扣、鞋花饰扣、包和、鸡眼、撞钉、爪珠、烤漆扣、水钻、压克力、玻璃钻石等。

This system can change the synthetic wavelength chain according to the distance to be measured. The inphase state of the interference signals, which is difficult for phase measurement, can be avoided by changing the wavelength freely. In addition, this system can realize the dimension calibration between the workpiece and the gauge block at a shortest measuring chain.

该测量系统可以根据待测距离选择合成波长链完成由粗测到精测的过程、通过波长的调谐能够避免零相位的测量，并在测量链最短的情况下实现了被测量和量块的直接比对，适用于孔径、平行平面间距的测量。

Geordie will be hanged in a golden chain ,'Tis not the chain of many .

喔我的乔第将以一条金色锁链被处以绞刑，那不是一条普通的绞链。

O my Geordie will be hanged in a golden chain,'Tis not the chain of many.

她在唱：喔，我的乔第将以一条金色锁链被处以绞刑，那不是一条普通的绞链；他出身皇族，不慎失足于一位贤淑女子。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新