查询词典 chain reaction

Kg1?d1 orally since the age of 8 weeks and the rats in the other two groups were given distilled water orally. body weights of rats were recorded weekly and blood glucose concentration was determined by oral glucose tolerance test. pancreas weights were recorded after rats were killed and the expression levels of bcl2 mrna and bax mrna were detected by reverse transcriptasepolymerase chain reaction.

每周记录大鼠体质量；采用口服葡萄糖耐量试验监测血糖；定期处死大鼠，分离胰腺并称重；采用逆转录聚合酶链反应检测bcl2和bax在胰腺组织中的表达。

The aliquots of the amplified DNA by polymerase chain reaction were electrophoresed on 1. 5% agarose gel and visualized by ethidium-bromide fluorescence.

将提取的适量RNA与逆转录酶、下游引物反转录合成cDNA的第一链，快速离心混匀，37℃1小时，95℃10分钟，灭活逆转录酶。

Vas deferens Abnormalities Gene Mutation Cystic fibrosis Polymerase chain reaction

输精管；畸形；基因；突变；囊性纤维病；聚合酶链反应

Crohn's disease was induced by trinitrobenzene sulfonic acid in the model group. The mice were sacrificed 5 days afterwards and LPMC were isolated and cultured. The expression of CCR2 and CCR5 on LPMC were determined by flow cytometry. The secretion of MIP-1α in the supernatant was determined by enzyme-linked immunosorbent assay, and the expression of MIP-1α mRNA was determined by reverse transcriptase polymerase chain reaction.

模型组小鼠以三硝基苯磺酸灌肠造模。5d后处死，提取和培养LPMC，采用流式细胞仪测定小鼠LPMC中趋化因子受体CCR2、CCR5的表达；采用酶联免疫吸附测定检测培养上清液中MIP-1α的分泌；采用逆转录聚合酶链反应检测MIP-1α mRNA的表达。

Flow cytometry techniques was used to to determine cell apoptosis under the action of mifepristone. And expression of survivin gene was evaluated by reverse transcription polymerase chain reaction. Result: Four different condensations dose-dependently inhibited the proliferation of HO8910 cells.the inhibition by mifepristone plateaued at 20 y.

将HO8910细胞进行体外培养，采用MTT法测定，观察不同浓度米非司酮(20，10，5，2.5μmol／L)对HO8910细胞的杀伤活性；通过流式细胞仪分析用药后细胞凋亡的情况；逆转录PCR法检测了米非司酮培养前后Survivin扣只人A的表达，并将结果进行了相关分析。

AIM: To establish a simple and fast hepatitis B virus covalently closed circular DNA detecting method based on polymerase chain reaction with satisfactory sensitivity and specificity.

摘要目的：建立一种基于聚合酶链反应的简便快速、具有较高敏感性和特异性的检测乙型肝炎病毒共价闭合环状DNA的方法。

After 5 days of coculture, the percentage of CD4 CD25 T cells was detected by flow cytometry. T cell proliferation was assessed by ［3H］ thymidine incorporation using a liquid scintillation counter. The expression of Foxp3 in CD4 CD25 T cells was detected by reverse transcription polymerase chain reaction.

众多学者对其可能的作用机制进行了研究，目前认为可能通过与免疫细胞（如T细胞，树突状细胞，B细胞等）直接接触［3］或通过改变细胞因子分泌网络间接影响免疫细胞发挥效应有关，但确切机制仍不清楚。

Objective To explore the role of bacteria in chronic prostatitis by detecting the bacterial 16S ribosomal RNA gene of the expressed prostatic secretion using polymerase chain reaction.

目的 通过采用聚合酶链反应技术对慢性前列腺炎患者前列腺液中细菌16S rRNA基因检测，探讨PCR方法诊断慢性前列腺炎细菌学病因的价值。

Objective: To evaluate the clinical value of fluorescent quantitative polymerase chain reaction in the diagnosis of primary syphilis.

目的为评价荧光定量聚合酶链反应在一期梅毒诊断中的临床应用价值，以暗视野、血清学检查方法作对照，用FQ-PCR方法检测68例疑诊为一期梅毒病人的生殖器溃疡处分泌物。

The fulllength humanized 13D4 scFv gene fragments were synthesized with splicing overlap extensionpolymerase chain reaction.

这些人源化抗体将大大降低13D4用于治疗人感染H5N1时的HAMA反应，为禽流感治疗性抗体的临床应用奠定基础。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。