查询词典 chain reaction

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

Methods: Genotyping of HLA-DRB1 was performed in 65 well-characterized PBC patients and 431 healthy controls with polymerase chain reaction-sequence-specific primers.

利用序列特异性引物PCR对65例确诊为PBC的患者和431例健康人进行HLA-DRB1等位基因以及有关基因亚型分析。

Methods:Genotyping of HLA-DRBl was performed in 65 well-characterized PBC patients and 431 healthy controls with polymerase chain reaction-sequence-specific primers.

随着临床医生对该病认识的逐步深人和实验室检查手段的不断提高，近年来国内外对PBC的报道均明显增加川。

Fortunately there is a way to control the chain reaction of uranium atoms splitting each other.

幸运地是有一种方法去控制链式反应中铀原子的相继裂变。

Methods Allele Specific polymerase chain reaction and Confronting Two-pair primers-PCR techniques were used to determine β_2-AR genetic polymorphisms in 42 patients with asthma,which were treated in The Affiliated Hospital of Inner Mongolia Medical College from 2004 July to 2005 September and 30 healthy controls.

方法对2004年7月至2005年9月内蒙古医学院附属医院收治的42例支气管哮喘患者和30名健康者采用等位基因特异性聚合酶链反应和两对相对引物PCR法(confronting two-pair primers，PCR-CTPP)进行β2-AR基因多态性分析。

The mice in experimental group and control group were exposed to 350 cGy radiation produced by 60Co. After 3 h, karyocytes at different concentrations in the fresh human umbilical cord blood were injected into the mice in experimental group A, B, C via their tail veins, and the equal volume of normal sodium was also injected into control group via tail veins. After one month, carbon tetrachloride (CCl4) was injected into experimental group A, B and control group via abdominal cavity, and the equal volume of normal sodium was injected into experimental group C. After two months, immunohistochemistry and reverse transcriptase polymerase chain reaction were used to detect the expressions of human cytokeratin-18 (CK18), cytokeratin-19 (CK19) and albumin in liver tissues of all mice.

采用60Co治疗仪γ射线对实验组和对照组行350cGy的亚致死剂量照射，实验组A、B、C3组照射后3h内经尾静脉分别输入1.0×10^7个/只、2.0×10^7个/只和3.0×l0^7个/只人新鲜脐血有核细胞，对照组经尾静脉注入等体积无菌生理盐水。1个月后对实验A、B组和对照组裸鼠经腹膜腔注射四氯化碳(CCl4)，实验C组注射等体积生理盐水。2个月后采用免疫组化和RT-PCR方法检测裸鼠肝组织人源性CK18、CK19和人源性白蛋白的表达。

I wanted to stay a moment of spiritual chain reaction, but unfortunately the sun sank, deepening twilight.

我本想在灵琐停留片刻，无奈太阳下沉，暮色苍茫。

Methods The human cytogegalovirus in blood serum of 176 confirmed pathological jaundice neonates was detected with polymerase chain reaction.

采用聚合酶链反应技术对176 例被确诊为病理性黄疸新生儿患者进行血清巨细胞病毒检测，并同时进行G6PD、血常规、胆红素等各项指标的测定，进行比较。

In this report, half-esterification between these pendent hydroxyl groups of hydroxyl-PU and maleic anhydride could lead to new pendent groups, carboxylic groups. These highly polar carboxylic groups in the side chain of PU could play the role of internal emulsifier. They had self-dissociation ability in the water or were neutralized first by appropriate amount of base. Then, PU bearing carboxylic group could dispersed steadily in the water, named aqueous-based polyurethane dispersion.

在本论文中，利用原来亲油性Hydroxyl PU主链段上悬挂的羟基经由与琥珀酸酐的半酯化(Half-esterification Reaction)反应后，使PU在侧链上悬挂具有强极性的羧酸基，其可作为聚胺酯分子中的内乳化剂，羧酸基能在水相中自身解离产生离子或加入适量碱使之中合，使聚氨酯高分子能够稳定分散於水中，即所谓水性PU分散液(Aqueous-based Polyurethane Dispersion)。

Methods: Expression of CK19mRNA in bone marrows of 48 breast cancer patients sand 20 benign breast disease patients were detected by reverse transcriptionpolymerase chain reaction.

作者：作者：黄艳春，阿先古丽·阿不力孜，封敏作者单位：新疆医科大学附属肿瘤医院检验科，新疆乌鲁木齐830011 来源：医学期刊/大学学报收藏本文章

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。