查询词典 chain reaction

The uptake of 〓I-OxLDL in adipocytes was determined by gamma ray counter and normalized to protein concentration of each sample, was expressed as nanograms of 〓I-OxLDL uptake per milligram of cell protein. Reverse transcription polymerase chain reaction was used to evaluate PPARγ and CD36 mRNA expressions.

采用酶联免疫吸附法检测血浆IL-6以及脂肪细胞分泌IL-6的水平；采用gamma射线计数仪来检测脂肪细胞对〓I-OxLDL的特异性摄取情况，采用改良Lowry法进行细胞蛋白浓度测定，细胞对〓I-OxLDL的特异性摄取量以ng／mg细胞蛋白表示；采用逆转录聚合酶链式反应来检测PPAR γ和CD36 mRNA在脂肪细胞的表达情况。

Reverse Transcription polymerase chain reaction with RNA PCR Kit,the product of RT-PCR was determined by electrophoresis of agarose gel (100v, 30s ),the result was shoot by GDS gel imaging system, the staps were scaned and quantitated by computer analysis system.

用RNA PCR试剂盒进行逆转录聚合酶链式反应，RT-PCR产物在1.2%琼脂糖凝胶上电泳(100V，30 min)，用GDS凝胶成像系统拍摄电泳结果，各带用计算机图像分析系统扫描定量。

These results revealed that the prepared serum and relevant assay could be used for serotyping field isolates.

多聚酶链式反应(Polymerase Chain Reaction，PCR)由于具有敏感快速的优点，已被成功应用于许多病原的快速检测和疾病诊断中。

Development of a quantitative competitive polymerase chain reaction assay for serotype 1 Marek's disease virus .

4株鸡马立克氏病病毒国内分离株Meq基因的克隆与序列分析。

Methods: Polymerase chain reaction/sequence specific primer technique was used to analyze HLA-DRB1 alleles in the following four groups: patients with chronic hepatitis B, carriers of HBV, spontaneous recoverers after HBV infection, and normal controls.

采用聚合酶链反应/序列特异性引物技术分别对慢性乙型肝炎组、HBV携带组、HBV感染后自然恢复组和正常对照组进行HLA-DRB1等位基因的检测。

HIV proviral DNA present in the peripheral blood mononuclear cells from the patient and his wife was amplified by a nested polymerase chain reaction and sequenced directly.

艾滋病毒基因存在于外周血单个核细胞，从病人和他的妻子被扩增一聚合酶链反应和直接测序。

HIV proviral DNA present in the peripheral blood mononuclear cells from the patient and his wife was amplified by a nested polymerase chain reaction and sequenced directly.

艾滋病毒基因存在于外周血单个核细胞，从病人和他的妻子被扩增一聚合酶链反响和直接测序。

A method for detection of proviral human immunodeficiency virus type 1 DNA fragment encoded pol gene in dried blood spots on filter paper by nested primers polymerase chain reaction has been developed.

目的建立套式引物聚合酶链反应，用于检测滤纸干血斑中的人免疫缺陷病毒1型（HIV1）前病毒pol基因DNA片段。

To test this concept in transplant recipients, we evaluated complement, complement regulator, and T cell/proinflammatory marker gene expression by quantitative real-time polymerase chain reaction in 71 archived heart transplant biopsies and correlated the results with the histologic grade of rejection. Significantly more transcripts encoding alternative pathway components factor B, C3 and properdin, and C3a receptor and C5a receptor were detected in grade 3 versus grade 0 or 1 biopsies. The grade 3 rejections also contained significantly higher amounts of CD3, interferon gamma, perforin, and granzyme B genes.

为了明确移植受者中该理论的正确性，我们采用RT-PCR的方法检测了71例心脏移植物活检标本中补体、补体调节物以及T细胞/促炎症反应标记物基因表达及其与排斥反应的组织学分级的相关程度。3级的排斥反应相比于0或者1级的排斥反应其活检标本中旁路途径成分因子B、C3以及备解素和C3a与C5a的受体表达均明显升高。3级排斥分那英同样存在更多的CD3、IFN-gamma、穿孔素和颗粒酶B基因的表达。

At the end of this paper, the development of polymerase chain reaction for the future is discussed.

最后简单介绍了聚合酶链式反应并展望了微生物采油技术的前景。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。