查询词典 chain reaction

Methods 966 cases of high risk female were examined by using the ligase chain reaction and investigated by questionnaire.

方法采用连接酶链反应和问卷对966例高危女性患者和527例对照组进行生殖道沙眼衣原体检测及相关高危因素调查。

Objective To study the difference between ligase chain reaction and immunochromatography assay in detection of Chlamydia trachomatis.

目的 比较连接酶链反应与免疫层分析法检测沙眼衣原体的差异。

Breast tissues were taken at surgery from patients with breast adenoma, nodule of lobuli glandulae mammariae and breast cancer. Normal samples adjacent to breast cancer were used as control. NIS mRNA and protein were assessed in these samples by reverse transcription polymerase chain reaction , Western blot and immunohistochemistry methods.

1。乳腺组织取自乳腺纤维瘤、乳腺小叶增生以及乳腺癌患者，另取癌旁正常乳腺组织作为对照，分别运用半定量逆转录聚合酶链反应、Western Blot方法和免疫组织化学技术检测NIS的基因和蛋白表达情况。

METHODS By nested polymerase chain reaction, the inactivation efficacy of three different concentrations of iodophor, potassium permanganate, Lysol,"84" disinfectant and jingxin liquid disinfectant was detected after TTV having been disinfected for 10, 30, 60 and 90 minutes by these disinfectants respectively.

采用巢式聚合酶链反应技术，检测 3种不同浓度的碘伏、高锰酸钾、煤酚皂液、&84&消毒液和金星消毒剂，对 TTV DNA分别消毒处理 10、30、6 0 min和 90 m in的灭活效果。结果碘伏在常规使用浓度(0 。5 %～ 1%)对 TTV DNA灭活效果好；高锰酸钾、来苏尔、&84&消毒液和金星消毒剂在高浓度下可灭活 TTV DNA ，而常规使用浓度对 TTV DNA灭活效果不好。

Observe the change cardiac rhythm and rate;The expression of GimRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针异搏定所致的缓慢性心律失常模型大鼠双侧&内关&穴，观察针刺前后心律变化；用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白mRNA的相对含量。

Observe the change cardiac rhythm and rate;The expression of Gi-mRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针乌头碱所致的快速性心律失常模型大鼠双侧&内关&穴，观察针刺前后心律变化；用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白mRNA的相对含量。

Methods: Arrhythmia was induced with cordilox, and acupuncture two-aide "Neiguan" by electro-acupuncture later. Observe the change cardiac rhythm and rate; The expression of G mRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针异搏定所致的缓慢性心律失常模型大鼠双侧&内关&穴，观察针刺前后心律变化；用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白 mRNA的相对含量。

Methods: tachyarrhythmia was induced with aconitine, and acupuncture two-side "Neiguan" by electro-acupuncture later. Observe the change cardiac rhythm and rate; The expression of G-mRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针乌头碱所致的快速性心律失常模型大鼠双侧&内关&穴，观察针刺前后心律变化；用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白mRNA的相对含量。

We draw fresh blood with EDTA anticoagulant of two millilitre from every patients. First we draw DNA of leukocyte from blood by the method with saturation phenol and chloroform second we amplificate the ACE gene using the DNA polymerase chain reaction technic employing the primer gene designed by Rigat.

首先应用饱和酚-氯仿法提取白细胞DNA，之后采用Rigat等人设计的ACE基因引物，应用PCR技术对标本ACE基因进行扩增，扩增产物采用2%琼脂糖凝胶电泳。

The coding region in the genomic RNA of the Maize Dwarf Mosaic Virus was gained by the way of reverse transcription polymerase chain reaction.

本研究利用RT-PCR对玉米矮花叶病河南标样的基因组RNA进行了全序列测定，并与Potyvirus于中其它病毒进行了同源性分析。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。