查询词典 chain reaction

In vivo footprinting is a method of studying DNA-protein interaction. It can reflect the authentic status of DNA-protein in vivo, and can also detect the change of DNA conformation. The introduction of Ligation-Mediated PCR greatly improves the sensitivity and specificity of in vivo footprinting study, and has facilitated the executation of in vivo footprinting in the regulation of eucaryotic gene expression.

体内足迹法是一种研究DNA-蛋白质相互作用的方法，它能真实反映体内蛋白-DNA相互作用的情况，而且可以检测体内的DNA构象的变化，连接介导的聚合酶链反应(Ligation-Mediatedpolymerase chain Reaction，LM-PCR)的发明，使得体内足迹研究的敏感性和特异性大大提高，有力地促进了体内足迹技术在真核基因表达调控研究方面的应用。

Exons 12 and 23 of the ABCB4 gene were amplified using polymerase chain reaction.

目的：探讨ABCB4基因外显子12和23的突变与妊娠期肝内胆汁淤积症发病的关系。

Reverse transcription polymerase chain reaction and western blot were used to detect the COCH and POU3F4/brn4 gene's expression. To investigate in vitro characteristics of COCH gene's expession, two factors were introduced in the culture medium of spiral ligament type Ⅰ fibrocytes respectively. Firstly, phosphorothionate-modified antisense oligodeoxynucleiotides to POU3F4/brn4 mRNA was applied to study the regulation effects of POU3F4/brn4 to COCH gene transcription.

引入人工合成的针对POU3F4／brn4基因mRNA的反义硫代磷酸寡聚脱氧核苷酸，干扰耳蜗螺旋韧带Ⅰ型成纤维细胞POU3F4／brn4的表达，免疫印迹检测反义干扰细胞POU3F4／brn4蛋白条带的强弱，RT-PCR检测反义寡核苷酸干扰细胞COCH基因转录水平的变化，观察POU3F4／brn4对COCH基因的转录调控作用。

The free neutron s can bombard other nuclei, leading to a series of fissions called a chain reaction.

自由中子可以轰击其它的核，导致一系列的裂变，称为链锁反应。

The free neutrons can bombard other nuclei, leading to a series of fissions called a chain reaction.

自由中子可以轰击其它的核，导致一系列的裂变，称为链锁反应。

Methods Cells were maintained in DMEM medium with 10% fetal bovine serum. Five days before the beginning of experiments, the cells were seeded in phenol red-free DMEM medium containing 5% charcoal dextran-treated FBS. The cells were harvested, and seeded in 6-well culture plates or in 75ml flacks. After NP, BisA and DBP treatments for 72h, the cells were harvested, and mRNA and protein expression of PCNA, bcl-2 and bax were detected by reverse transcription-polymerase chain reaction and immunohistochemistry, respectively.

方法］T47D细胞在含10%胎牛血清的DMEM培养液中进行常规传代培养，实验前将细胞转移至无酚红DMEM（含5%活性碳葡聚糖苷处理过的FBS）中继续培养5d，收集细胞，PBS洗涤后接种於内置盖玻片的6孔板或75ml培养瓶中，用32×10^(-7)mol/L NP、32×10^(-7)mol/L BisA及32×10^(-6)mol/L DBP对细胞分别处理72h，用半定量RT-PCR技术观察这3种化合物对核增殖抗原PCNA、bcl-2及bax mRNA表达的影响，并用免疫组化方法对结果进行验证。

Methods: Polymerase chain reaction was used in the study of GSTP1 genotype, than the discrepancies of GSTP1 genotype frequences between 56 UC patients and 120 healthy controls were anatysed by χ^2 test.

用聚合酶链反应技术检测56例溃疡性结肠炎和120例健康人群的GSTP1基因型，采用χ^2检验，分析比较GSTP1基因型频率在UC组和健康对照组之间的分布差异。

Methods:Polymerase chain reaction was used in the study of GSTP1 genotype, than the discrepancies of GSTP1 genotype frequences between 56 UC patients and 120 healthy controls were anatysed by x2 test.

用聚合酶链反应技术检测56例溃疡性结肠炎和120例健康人群的GSTP1基因型，采用x2检验，分析比较GSTP1基因型频率在UC组和健康对照组之间的分布差异。

Objective To evaluate the value of ligase chain reaction in the diagnosis of gonorrhoea.

目的 评价连接酶链反应技术对淋病诊断的意义。

Objective To detect the DNA of Neisseria gonorrhoeae in clinical genitourinary specimens by the real-time fluorescence quantitative Polymerase Chain Reaction , and to provide a reliable support for the clinical diagnosis and treatment of gonorrhoea.

目的 应用实时荧光定量聚合酶链反应技术准确定量检测临床标本中淋病奈瑟菌基因，同时与金标准—细菌培养法比较其敏感性和特异性，为临床淋病的诊疗提供依据。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。