查询词典 chain reaction

Years old, 25 being colonic cancer,51 being rectal cancer, 31 had limph-node metastasis, 13 had distant metastasis.In the present study, 44 cases of gastric cancer with normal gastric mucosa and 62 caese of colorectal cancer with there normal mucosa was detected CD44v6 raRNA expression using reverse transcription-polymerase chain reaction.

结果 胃、大肠的正常动膜有少量CD44V6表达：胃癌、大肠癌组织CD44V6VRNA、蛋白的表达率均高于其正常锚膜（P＜0.00），44例胃癌组织 CD44V6IuRN表达阳性率在不同的性别、发生部位、大体类型、组织类型、组织学分化程度、浸润深度和TNM分期间差异无显著性。

Reverse transcription-polymerase chain reaction was used to detect MNV in the mouse cecal contents.

结果共检测小鼠盲肠内容物206份，阳性样本为77份，阳性率为37.38%。3个设施的小鼠感染MNV，各品系小鼠易感性差异无显著。

Methods Hepatic fibrosis models of rabbits were made by appling abdomen walls with the cercarias of Schistosome japonica.The rabbits were randomly categorized into four groups:normal control,4,8,12 weeks after application.The level of transforming growth factor-beta1(TGF-β1) mRNA in hepatic tissues was examined by reverse transcription polymerase chain reaction. The level of expressions of Smad3 was examined by Western blot. Meanwhile,immunohistochemistry staining was assessed by measuring type Ⅰ collagen .

日本血吸虫尾蚴腹贴法制备家兔肝纤维化模型(n =32 )，按诱导时间将动物随机分为 4、8、12周及正常对照共 4组，采用逆转录多聚酶链反应检测肝组织中转化生长因子TGF -β1mRNA表达水平，免疫组织化学法检测Ⅰ型胶原的表达，同时应用Westernblot检测Smad3蛋白的表达。

The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction from the genomic DNA ofM.tuberculosis H37RV strain;(2) To clone PCR product of Tb wbbLgene into a cloning vector pMD18-T for sequencing;(3) To subcloneTb wbbL gene to an expression vector pET16b to construct pET16b-Tb wbbL; and to overexpress Tb WbbL protein in E.coli BL21(DE3)under different induction conditions;(4) To establish theco-expression system for expressing chaperons of pKJE7 plasmidand soluble Tb WbbL protein in E.coli BL21(DE3) under differentinduction conditions;(5) To test expressed WbbL protein by SDS-PAGEand Western blot methods.

本论文的目的是：(1)利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因；(2)将 Tb wbbL 基因克隆到pMD18-T 克隆载体中，经 DNA 序列测定证实为正确的基因；(3)将 Tb wbbL 基因亚克隆到 pET16b 表达载体中并通过改变不同的诱导条件在大肠杆菌 BL21(DE3)中表达 Tb WbbL 蛋白质；(4)建立在 BL21(DE3)大肠杆菌中共表达 Tb WbbL 蛋白质与分子伴侣的体系，优化表达条件以高效表达出可溶性 Tb WbbL蛋白质；(5)用 Western blot 方法鉴定所表达的 Tb WbbL 蛋白质为 Tb wbbL 基因产物。

Methods To detect GPⅢ a P1^A polymorphism in 100 cerebral thrombosis patients and 100 healthy collators with polymerase chain reaction restriction fragment length polymorphismtechnique.

方法运用聚合酶链反应-限制性片断长度多态性分析技术检测100例脑血栓患者及100例健康对照者GPⅢa的P1^A的多态性。

Noninvasive ambulatory blood pressure monitoring and genotyping by polymerase chain reaction amplification of all subjects were performed. The patients were then classified into four groups according to their dipping status: extreme dippers, dippers, non-dippers, and risers.

参与研究之高血压患者依其二十四小时血压纪录分为四类型：夜间血压下降极明显者、夜间血压下降明显者、夜间血压下降不明显者(non-dippers)、和夜间血压上升者。

Set up they different dominos in such a way that they all fall down inan exciting nuclear chain reaction.

通过摆弄这些不同的骨牌，使它们能在多米诺链中全部倒下！

A little over four million dominos were knocked down in a chain reaction.

略大于400万张的多米诺牌在连锁反应的作用下被推倒。

What wor-ries Hudnut is the possibility of the geological equivalent of dominos: What if an earthquake on one fault causes a chain reaction

赫德纳特担心的是地质学上发生骨牌效应的可能性：万一某一断层的地震引起连锁反应？

The introduction of Ligation-Mediated PCR greatly improves the sensitivity and specificity of in vivo footprinting study, and has facilitated the executation of in vivo footprinting in the regulation of eucaryotic gene expression.

DNA相互作用的情况，而且可以检测体内的DNA构象的变化，连接介导的聚合酶链反应(Ligation-Mediated polymerase chain Reaction，LM-PCR)的发明，使得体内足迹研究的敏感性和特异性大大提高，有力地促进了体内足迹技术在真核基因表达调控研究方面的应用。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。