查询词典 chain reaction

Objective:To establish a real-time quantitative reverse transcription polymerase chain reaction method for examining the expression of sialic acid-binding immunoglobulin-like lectin-one (Siglec-1)gene in the peripheral blood mononuclear cells in patients with coronary heart disease,so as to explore the relationship between Siglec-1 mRNA expression and the development/progression of CHD.

目的：建立检测人Siglec-1(sialic acid-binding immunoglobulin-like lectins，唾液酸结合的免疫球蛋白样凝集素1，CD169)mRNA 含量的实时荧光定量RT-PCR 的方法，并用来测定冠心病患者外周血单个核细胞中Siglec-1的基因表达水平，探讨Siglec-1基因表达水平与冠状动脉粥样硬化发生发展的关系。

According to different length of gene encoding HSP150 band amplified by polymerase chain reaction, 15 strains could be divided into 6 genetic types.

综合2种分析结果，并对低双乙酰酿酒酵母菌株YZU—APK和SS-05进行DNA分子标记，确定了其基因型分别是（P07：1，P42：1，hsp150：1）和（P07：1，P42：5，hsp150：2）。

Especially serious is that of a "grass baskets" in conjunction with the fate of a number of "grass basket", so he caused a chain reaction, a large number of enterprises can not repay short-term debt as a result of the collapse into bankruptcy and the state.

尤其严重的是，一个&草筐&的命运联着若干个&草筐&，于是就造成了连锁反应，大量企业由于不能偿还短期债务而陷入倒闭破产的境地。

This study paid more attention to the techniques of detecting Soybean mosaic virus in dry soybean seed and Bean common mosaic virus in dry bean seed by reverse transcription-polymerase chain reaction, combining with biological test and enzyme-linked immosorbent assay. The goal of the research is to establish a molecular detection procedure of SMV and BCMV from dry seed. The main results are following:⑴The effective techniques were made to extract total RNA of SMV from dry soybean seed and BCMV from dry bean seed.

本研究以大豆和菜豆种子中主要种传病毒大豆花叶病毒、菜豆普通花叶病毒作为检测对象，在生物学测定和酶联免疫吸附测定的基础上，研究干种子中两种病毒的逆转录-聚合酶链式反应检测方法，以建立一套快速、灵敏、专化性强的直接以干种子作为检测对象的分子检测技术体系，主要结果如下：⑴建立了从大豆和菜豆干种子中提取SMV、BCMV总RNA的有效方法。

To establish delta block HLA-matching technique,DNA was extracted from whole blood by salting-out method,delta block was amplified by polymerase chain reaction,and PCR product was detected by GeneScan.

为了建立delta模块配型的检测方法，采用盐析法提取DNA，PCR技术扩增delta模块，GeneScan模式检测PCR产物。

Methods: 70 patients with COPD and 70 healthy volunteers were enrolled in this study. Limosis peripheral blood was taken and whole blood corpuscle genomic DNA was extracted. The genetic polymorphism of SOD1-3 genes was determined by polymerase chain reaction and restriction fragment length polymorphism.

收集70例COPD患者和70例健康人空腹外周血，提取全血细胞DNA，采用聚合酶链式反应－限制性片段长度多态性法检测两组SOD1-3基因的多态性。

DNA vaccine; genital gland and blood; residue level; fluorescent quantative polymerase chain reaction

本研究的目的是建立一种快速准确的检测灵长目类动物生殖腺和血液中DNA疫苗残留拷贝的方法，为疫苗进入一期临床实验和进行安全性评价奠定良好基础。

Borna disease virus ; Reverse transcription-polymerase chain reaction ; Receptor ; Serotonin ; Dopamine ; Transcription ; genetic

Borna病病毒；逆转录聚合酶链反应；受体；血清素；多巴胺；转录；遗传

Objective This study aimed to establish a fast and accurate quantitative method to detect Borna disease virus ribonucleic acid using fluorescence quantitative nested reverse transcriptase polymerase chain reaction.

目的：本研究拟建立博尔纳病病毒荧光定量套式逆转录酶聚合酶链反应检测方法，为快速、准确地定量检测 BDV 奠定基础。

Objective This study aimed to establish a fast and accuratequantitative method to detect Borna disease virus ribonucleic acid using fluorescence quantitative nested reverse transcriptasepolymerase chain reaction.

目的：本研究拟建立博尔纳病病毒荧光定量套式逆转录酶聚合酶链反应检测方法，为快速、准确地定量检测 BDV奠定基础。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。