查询词典 chain reaction

Ovarian neoplasma ; Multiple drug ; Resistance ; Gene expression ; Polymerase chain reaction

卵巢肿瘤；抗药性；多药；基因表达；聚合酶链反应

This was done using a high-performance liquid chromatography system equipped with an electrochemical detector, endonuclease nicking assay and reverse transcription polymerase chain reaction, respectively. RESULTS: H2O2 induced the formation of 8-oxo-dG in DNA at concentrations of 100 and 200 μmol/L.

结果：与对照组比较，100、200、300μmol/LH2O2浓度时均能使A549细胞DNA的8-oxo-dG水平增高（P.05或P.01）。200μmol/LH2O2作用12h后8-oxo-dG水平上升到峰值并在24h降至基线水平，8-oxo-dG水平在6~18h处理组显著高于对照组（P.05或P.01）。

Reverse transcription polymerase chain reaction was used to examine the deletions at P16 and FHIT in 52 patients with lung cancer and 9 patients with nonmalignant disease.

采用逆转录——聚合酶链反应方法，对52例肺癌患者和9例肺良性疾病患者的纤支镜活检标本中P16和FHIT基因缺失情况进行检测。

METHODS: Twentyfour patients with congenital heart defects were divided into 3 groups: mild pulmonary hypertension group(n=8), moderate or severe pulmonary hypertension group(n=9), and nonpulmonary hypertension group(n=7). NOS mRNA expression was detected in lung tissues with reverse transcriptase polymerase chain reaction.

将24例左向右分流先天性心脏病患者分为三组：轻度肺动脉高压8例，中重度肺动脉高压9例和无肺动脉高压7例，应用逆转录多聚酶链式反应检测患者肺组织中NOS mRNA的表达。

Methods Blood samples were obtained from 5 patients with AIDS, the C2~C3 region of HIV-1 envelope gene was amplified by nested polymerse chain reaction from plasma of each patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous and nonsynonymous substitutions as well as ds/dn ratios were calculated.

采用套式聚合酶链反应方法对5例艾滋病患者血浆HIV-1 env基因C2~V3区进行PCR扩增，产物经纯化后克隆至pMD-18T载体中，每例患者分别挑取5~8个克隆，共34株，完成阳性克隆的鉴定、抽提纯化和测序，将获得的DNA序列及核苷酸序列进行分析，并计算其核苷酸同源替换率与非同源替换率的比值。

Blood samples were obtained from 5 patients with AIDS, the C2~C3 region of HIV-1 envelope gene was amplified by nested polymerse chain reaction from plasma of each patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous and nonsynonymous substitutions as well as ds/dn ratios were calculated.

采用巢式PCR方法对5例艾滋病患者血浆HIV-1env基因C2~V3区进行PCR扩增，产物经纯化后克隆至pMD-18T载体中，每例患者分别挑取5~8个克隆，共34株，完成阳性克隆的鉴定、抽提纯化和测序，将获得的DNA序列及氨基酸序列进行分析，并计算其氨基酸同源替换率与非同源替换率的比值。

Infertility ; male ; Oligospermia ; Gene deletion ; Y chromosome ; Polymerase chain reaction

不育；男性；少精子症；基因缺失； Y染色体；聚合酶链反应

G banding karyotype analysis of peripheral lymphocytes in 217 patients with azoospermia or severe oligospermia were performed and the Y-chromosome AZFc region from seven cases with Y chromosome abnormality was amplified by polymerase chain reaction.

对217例无精和严重少精症患者外周血淋巴细胞染色体核型进行分析，并采用聚合酶链反应对7例Y染色体结构异常患者的AZFc区进行检测。

A polymerase chain reaction method was used to modify the growth hormone cDNA of Oncorhynchus tschawytscha.

采用聚合酶链反应方法改造鲑鱼生长激素cDNA。

METHOD: Fos ontogenic protein immunohistochemistry and double antigen immunohistochemistry method; semiquantitative reverse transcription-polymerase chain reaction method were used.

Fos 癌蛋白免疫组化LSAB 法结合双抗原标记法；半定量逆转录聚合酶链反应方法。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。