查询词典 chain reaction

Methods Differential display reverse transcription polymerase chain reaction was carried out to screen the differentially expressed gene fragments from the lateral geniculate body and the visual cortex.

利用差异显示反转录PCR技术分析大鼠外侧膝状体与视皮层基因差异表达情况，并筛选差异表达基因片段。

Gerbillinae ; DNA ; Polymerase chain reaction ; Genetics

沙鼠亚科； DNA ；聚合酶链反应；遗传

Four field strains of infectious bronchitis viruswere isolated from chicken kidney, trachea and lung of the young layer flocks in some regions of Shaanxi province. Biological characterizations of the virus were investigated by virus pathogenicity to chicken embryo and healthy chickens, hemagglutination test, hemagglutination inhibition test, dwarfing embryonated chicken eggs, electron microscopy and reverse transcription polymerase chain reaction. It demonstrated that allantoic fluid of each passage virus could hemagglutinate chicken red blood cells by treatment with 1% trypsin. The standard positive serum to IBV could inhibited the hemagglutination. The isolates could make respiratory and kidney diseases in chickens.

采集陕西省宝鸡市、扶风县、兴平市和榆林市等地鸡场疑似传染性支气管炎发病鸡病料，通过9～11 日龄非免疫鸡胚进行了病毒的实验室分离和传代，并对分离毒株进行了病毒的血凝性、血凝抑制性、致病性、鸡胚矮小化、电镜特征等生物学特性鉴定，结果表明，4 株分离株经1%胰酶处理后的各代病毒尿囊液均可凝集鸡红细胞，标准阳性血清可特异性的抑制其凝集性，回归试验可复制出与自然发病相同的病例，病毒传代物有明显的致鸡胚矮小化作用，透射电镜下可见有近似球形、直径100 nm左右的冠状病毒粒子，将分离鉴定的4 株IBV 定名为BJ-04、FF-04、XP-03 和YL-04。

TTV DNA isolated from sera of 6 hemophilic patients by polymerase chain reaction were cloned using TA easy vector.

目的 了解血友病人的输血传播病毒基因型及其在个体内存在状况。

TTV DNA isolated from sera of 6 hemophilic patients by polymerase chain reaction were cloned using TA easy vector. 3-5 clones selected from each patient randomly were sequenced, and then compared with TTV DNA sequences in GenBank.

对6例血友病人血清中分离的TTV进行基因克隆，从每例病人的标本随机选多个克隆进行测序，比较其核苷酸和氨基酸序列。

Partial cDNAs encoding LHR gene were cloned from Cynoglossus semilaevis by nested reverse transcrip-polymerase chain reaction (nested RT-PCR). Alignment analysis showed that the sequence was 97%, 94%, 85%, 83%, 82% and 80% similar to those of Hippoglossus hippoglossus, Paralichthys olivaceus, Acanthopagrus schlegelii, Oreochromis niloticus, Oryzias latipes and Takifugu rubripes, respectively.

采用巢式RT-PCR法克隆了半滑舌鳎Cynoglossus semilaevis促黄体激素受体(Luteinizing Hormonene Receptor， LHR)基因部分序列，经过BLAST比对，认为该序列与庸鲽、牙鲆、黑鲷、尼罗罗非鱼、青鳉和红鳍东方鲀的LHR基因同源性分别为97%，94%，85%，83%，82%和80%。

Histiocytic disorders ; malignant ; Polymerase chain reaction ; Receptor ; antigen ; T-cell ; Gene rearrangement

组织细胞增生症；恶性；聚合酶链反应；受体；抗原； T细胞；基因重排

One histiocytic sarcoma showed t(14;18) by quantitative-polymerase chain reaction, which was confirmed by fluorescence in situ hybridization analysis showing IGH/BCL2 fusions in neoplastic histiocytes.

定量性聚合酶链反应显示1例组织细胞肉瘤发生了t(14；18)，此结果也得到荧光原位杂交分析的证实，即肿瘤性的组织细胞发生了IGH/BCL2基因的融合。

The histochemical staining and polymerase chain reaction was used to detect oral leukoplakia.

应用组织化学染色法和聚合酶链反应技术对口腔白斑是否存在念珠菌进行检测。

We have developed a method for detection of Hypodermal and hematopoietic necrosis baculovirus by polymerase chain reaction.

皮下和造血器官坏死杆状病毒［1］、对虾杆状DNA病毒［2］和白斑杆状病毒［3］是近年来引起全球对虾暴发性死亡的病原。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。