查询词典 chain reaction

ObjectiveTo assess the application value of fluorescence quantitative polymerase chain reaction for detection of treponema pallidum DNA.

目的：评估荧光定量聚合酶链反应技术检测梅毒螺旋体DNA的应用价值。

AIM To study the effects of polymerase chain reaction and intensified kinyoun in the diagnosis of L-form tubercle bacillus infection.

目的 探讨L型抗酸菌的IK染色和PCR检测的临床应用价值。

Intimal areas were measured using morphometric analysis of perfusion-fixed vein graft specimens, and intimal thickness was calculated using circumferential measurements. The SMC proliferation was studied by the immunohistochemical detection of proliferating cell nuclear antigen. Expression of MMP-2、MMP-9 mRNA in vein grafts and unoperated control Vein grafts was detected by reverse transcription polymerase chain reaction. Substrate gel zymography was used to determine the proteolytic activity.

分别行HE染色、Verhoeff弹性纤维染色观察组织病理变化，计算机病理图象分析系统测量新生内膜厚度及面积，免疫组织化学方法检测静脉壁增殖细胞核抗原表达以观察细胞增殖情况，半定量逆转录-聚合酶链反应检测静脉壁基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)mRNA的表达，明胶酶谱法检测MMP-2、MMP-9活性，比较各组之间的差别。

Reverse Transcription-Polymerase Chain Reaction was used to clone the whole-length gene of BMP-2 and construct AdV vector.

应用反转录-聚合酶链反应法克隆出骨形态发生蛋白质类2全长基因，构建重组腺病毒载体，DNA-磷酸钙共沉法将辅助质粒PJM17转染293细胞，同源重组构建出重组腺病毒。

METHODS: MyoD cDNA fragments were extracted from plasmids pEMSV-MyoD with polymerase chain reaction, and PCR was used to clone the whole-length gene of MyoD. After adding CACC sequence at 5' end, MyoD gene was cloned by orient topology into transfer ventor, pENTR/D-TOPO. Objective gene was transferred into adenoviral expression vector DNA via pENTR/D-TOPO vector. The recombinant adenoviral vectors transfected into HEK293A cells by using lipofectamine were packaged and amplified.

从pEMSV-MyoD质粒上用聚合酶链反应法扩增出MyoD cDNA片段，再通过聚合酶链反应使MyoD基因加上CACC序列接头，经过定向拓扑克隆使目的基因连接到转移载体上，再通过LR酶促反应，将目的基因转移到腺病毒表达载体DNA上，获得MyoD基因重组的腺病毒DNA，用脂质体转染法转染HEK293A细胞，包装扩增出MyoD基因重组的腺病毒。

Methods With β-actin gene as a reference, reverse transcriptase polymerase chain reaction was conducted to dectect the expression of PSA mRNA in 35 BC samples, 12 paracarcinoma breast tissues and 10 breast adenofibroma samples.

以β actin作为内部参照半定量RT PCR方法检测 35例乳腺癌组织、12癌旁乳腺组织和 10例乳腺纤维瘤中PSAmRNA的表达。

METHODS: expression of Pgp and MDR1 mRNA were detected by immunohistochemistry and reverse transcriptionpolymerase chain reaction in the specimens from 25 breast cancer patients with neoadjuvant chemotherapy, 15 breast cancer patients without neoadjuvant chemotherapy and 10 patients with breast adenosis.

采用免疫组织化学及逆转录多聚酶链反应技术，对25例接受术前新辅助化疗的乳腺癌患者、15例未接受术前化疗的乳腺癌患者、10例乳腺腺病患者组织标本内Pgp 和MDR1 mRNA的表达情况进行检测。

METHODS: The expression of Pgp and MDR1 mRNA were detected by immunohistochemistry and reverse transcriptionpolymerase chain reaction in the specimens from 25 breast cancer patients with neoadjuvant chemotherapy, 15 breast cancer patients without neoadjuvant chemotherapy and 10 patients with breast adenosis.

采用免疫组织化学及逆转录多聚酶链反应技术，对25例接受术前新辅助化疗的乳腺癌患者、15例未接受术前化疗的乳腺癌患者、10例乳腺腺病患者组织标本内Pgp 和MDR1 mRNA的表达情况进行检测。

The player utilized the aimer to shoot the bubble towards a location in the cube. If more than a certain number of bubbles with the same color are connected, they explode through a chain reaction and the remaining bubbles that are not connected to the main layer fall.

如果超过一定数量的泡沫具有相同的颜色是相连的，他们可以通过一个连锁反应爆炸，其余的气泡，不连接到主层的就会掉下。

Adult male Wistar rats were randomLy allocated to 3 groups:control group, stone-forming group, Alisma Orientalis group. Reverse transcription polymerase chain reaction technique was used to examine bikunin mRNA expression levels in rat renal tissue.

将Wistar大鼠随机分成对照组、结石模型组、泽泻组，采用半定量逆转录聚合酶链反应检测大鼠肾组织bikunin mRNA的表达水平、镜下观察肾组织草酸钙晶体分布，同时检测大鼠血生化、肾钙含量、24h尿钙和尿草酸的分泌量。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。