查询词典 chain reaction

Inaddition,we extract total RNA from cultural U251 cell of humanglioma,make coding gene extron amplification of TfR through ReverseTranscription-Polymerase Chain Reaction,construct expressionplasmid of pCDNA3.1-TfR after digestion of enzyme andconjunction,do transfection to U251 cell by liposome afteridentification,cause excess expression,detect the efficiency of transientexpression of TfR protein by pEGFPN1-TfR, sieve positive cell ofpCDNA3.1-TfR through G418, choose positive cell clone, detect theexpression of TfR in anteroposterior period through Western blot,FCM,cell immunochemistry and mRNA through RT-PCR, identify the effect of transfection.

另外，培养人脑胶质瘤U251细胞，进行细胞总RNA抽提，运用RT-PCR方法进行TfR基因的完整编码区外显子的扩增，经过酶切、连接后构建pCDNA3.1-TfR的表达质粒，鉴定正确后按脂质体转染方法转染人胶质瘤U251细胞，使其过量表达，通过pEGFPN1-TfR检测其瞬时转染的效率；用G418对稳定表达pCDNA3.1-TfR的细胞进行筛选，挑选阳性细胞克隆，运用Western blot、流式细胞检测技术和细胞免疫化学以及mRNA半定量的方法检测TfR在转染前后的表达水平，鉴定转染的效果。

Objective To explore a simple and quick polymerase chain reaction method for the genotyping of Fmr1 knockout mice.

目的为Fmr1基因敲除小鼠探索快速、简单的基因型PCR检测方法。

Methods The template DNA was extract from the human osteosarcoma cells line U－2OS by the single- step isolation method with isothiocyanic acid guanidine, the cDNA coding for the mature fragment of BMP－4 was amplified by the reverse transcription- polymerase chain reaction.

方法采用异硫氰酸胍一步法从人骨肉瘤细胞株U-2OS中提取总RNA，通过逆转录-聚合酶链反应扩增为BMP-4成熟区cDNA基因片段。

Methods The mRNA and protein expression levels of NCAM were detected by reversed transcriptive polymerase chain reaction and Western blot after the rat hippocampal neurons were incubated with 20, 40 and 80μg/ml sodium fluoride for 24 hours in vitro.

原代培养海马细胞暴露于20、40、80μg/ml氟化钠24 h后，用RT-PCR和Western blot方法分别检测细胞内NCAM mRNA和NCAM各蛋白亚型的表达。

Weeks later, all the rats were killed, the levels of sodium pump α1-,α2-, and α3-subunit mRNA and protein in the cortex of kidney were detected with reverse transcription polymerase chain reaction method and immunohistochemical assay, respectively.

分别应用RT-PCR及免疫组化技术，探讨1k1c高血压大鼠肾脏皮质钠泵α1、α2及α3亚单位mRNA及蛋白水平基因表达的改变。

Weeks later, all the rats were killed and sodium pump α1-,α2-, and α3-subunit in aortic smooth muscles were detected with reverse transcription p ol ymerase chain reaction method and immunohistochemical assay at both mR NA and protein levels, respectively.

分别应用分子生物学 RT-PCR及免疫组化技术，探讨1k1c高血压大鼠动脉平滑肌细胞钠泵α1、α2及α3亚单位mR NA及蛋白水平基因表达的改变。

The Sonance Feedback Shell detonates and starts a chain reaction in a small area of effect.

其回音炮弹将在一定区域内造成连锁伤害。

If he could hit it, it might cause a chain reaction that would leave the ship dead in space.

如果他可以击中它，它可能引起一个将会离开空间的船死连锁反应。

This occurs in the core region of the star using the proton–proton chain reaction process.

这发生在太阳核区域的质子–质子链反应过程中。

Methods Blood cells of total of 77 cases with senile cataract and 76 controls were detected for GSTM1 gene, and the subcapsular epithelial cells of 22 cataract lenses were also detected for GSTM1 gene.

应用多聚酶链式反应（polymerase chain reaction， PCR）方法对77例老年性白内障患者及76例健康对照者外周血细胞进行GSTM1基因检测，并对其中的22例老年性白内障晶体上皮细胞进行GSTM1基因检测。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。