查询词典 chain reaction

The nucleoid size analysis, the comet assay and quantitative polymerase chain reaction.

细胞核大小分析，慧星检测法，PCR定量法。

In this paper,techniques of reverse transcription polymerase chain reaction and rapid amplification of cDNA ends have been used to clone a gene,which encodes a general odorant binding protein,from the antennae of Loxostege sticticalis.

利用RT-PCR结合RACE技术克隆了编码草地螟触角中一种普通气味结合蛋白的基因。

Methods Polymerase chain reaction and restriction fragment length polymorphism was used to identify mdm2 genotypes. The Pearson Chi square test and Woolf statistic method were used to analyze the relative risk and 95% confidence interval in order to find the genetic association between polymorphism of mdm2 gene and symptoms and pathological types of NSCLC.

采用聚合酶链反应和限制性内切酶片段长度多态性方法检测88例NSCLC患者的mdm2基因型，并通过Pearson卡方检验及Woolf方法计算相对风险度及95%可信区间（95%CI），验证mdm2基因多态性与各临床症状及病理类型之间的关联性。

The aim of this study was to analyze the relative expression patterns of mRNA for IGFs in different development stage of preimplanted ovine embryos by using reverse transcription-polymerase chain reaction.

本研究主要在mRNA水平上检测了IGFs在绵羊植入前不同发育阶段胚胎中的表达规律。

Pocine interferon α mature protein genes were amplified by reverse transcription polymerase chain reaction, and the recombinant replication-defective human adenovius serotype 5 plasmid pAd-poIFN-α was constructed. When the recombinant plasmid pAd-poIFN-α was linearized with PacI, and then transferred into HEK-293A cells, the virus plaque was isolated and purified in HEK-293A cells by three of plaque purification passages.

本研究利用RT-PCR方法扩增猪α干扰素成熟蛋白基因后构建了重组腺病毒质粒pAd-poIFN-α，经PacI酶切后转染HEK-293A细胞，3次噬斑纯化后获得了重组腺病毒rAd-poIFN-α。

Urogenital swabs were inoculated on the conventional solid media, new solid media and urea liquid media as such.⑤Synovial fluid, synovial-membrane biopsy samples, blood, urogenital swabs and pharyngal swabs from RA were examed the presence of mycoplasmas using culture and Polymerase Chain Reaction assay.

46例泌尿生殖道标本直接划在固体培养基表面后洗脱在Uu液体培养基和Mh液体培养基中；另20例标本直接划在无青霉素的固体培养基后洗脱在Uu液体培养基中。

We performed reverse transcriptase polymerase chain reaction in the 10th generation of treated cells to examine the expression of BDV phosphoprotein.

转染细胞经培养10代后仍然表达目的蛋白，成功建立稳定表达体系。

The test was divided into three groups randomly as yh-16 - experiment group, pbs- blank control group and rpmi-1640 medium - positive group.③in the following 24h、48h and 72h interventions, the morphous of ec was observed by photology inverted microscope, the cytoactive was detected by method of mtt and the cell cycle was investigated by flow cytometry.the expression of vegf was dectected by real-time fluorescent quantitate polymerase chain reaction(real-time pcr).

以有效浓度的yh-16作用于ec，在不同时相点光镜下观察ec形态，mtt检测细胞活性，流式细胞术检测细胞周期，实时荧光定量pcr检测vegf的表达量。

The major gene of CTXΦ receptor Tcp (toxin-coregulated pilus), tcpA, was detected by polymerase chain reaction analysis.

然而在本实验室研究中，于不含ctxAB基因的El Tor型霍乱弧菌中发现部分菌株含有RS基因同源序列，为此对这些菌株作CTXΦ基因组的进一步研究。

DETECTION OF PLASMODIUM VIVAX BY POLYMERASE CHAIN REACTION IN HAINAN .

应用聚合酶链反应在海南检测间日疟原虫的效果。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。