查询词典 chain reaction

Methods MHC-DRB1, DQA1, DQB1 were examined by using polymerase chain reaction/sequence specific oligonuleotide probe methods in 19 patients with miliary tuberculosis, 36 with tuberculous menigitis,20 with primary tuberculosis, 28 with tuberculous pleurisy and 91 controls.

采用聚合酶链式反应-序列特异性寡核苷酸探针斑点杂交方法，测定原发性肺结核20例、结核性胸膜炎28例、粟粒型肺结核19例、结核性脑膜炎36例和91例正常对照组的MHC-DRB1，DQA1，DQB1等位基因。

The I/D polymorphism of ACE was detected by polymerase chain reaction, and the CMA/B poly- morphism of Chymase gene was detected using PCR-restriction fragment length polymorphism.

运用聚合酶链反应检测 ACE基因的I/D多态性，运用限制性片段长度多态性技术检测Chymase基因的CMA/B多态性。

Methods The effect of ginsenoside-Ro on murine splenocytes proliferation was studied using [3H] thymidine incorporation assay. Effects of ginsenodide-Ro on the production of cyrokines interleukin-2 (IL-2), interferon-γ and interleukin-4 (IL-4) from mutine splenocytes were detected by ELISA method. Effects of ginsenoside-Ro on mRNA level of TH1 cytokine IFN-γ and Th2 cytokine IL-4 were evaluated by reverse transcription polymerase chain reaction analysis.

方法[3H] TdR参人法检测人参皂有－Ro对小鼠脾淋巴细胞增殖的影响；酶联免疫吸附法检测人参皂苷－Ro对小鼠脾淋巴细胞产生细胞因子白介素－2、干扰素－γ和白介素-4的影响；逆转录聚合酶链式反应分析法研究人参皂苷-Ro对小鼠脾淋巴细胞引干扰素－Υ、白介素-4mRNA表达的影响。

Nested polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples.

聚合酶链反应和DNA探针联合检测临床标本中结核杆菌的研究。

Methods Using polymerase chain reaction method,24 patients withNPC, 10 patients with other head and neck carcinoma(control group 1) and 10 patients with chronic nasopharyngitis(control group 2) were tested in the presence of Epstein Barr virus DNA in peripheral blood leukocyte and serum.

采用多聚酶链反应检测 2 4例鼻咽癌患者及 10例头颈部其它部位肿瘤(对照组 1)和 10例慢性鼻咽炎患者(对照组 2 )外周血白细胞及血清中EB病毒的DNA。

Methods Specimens of neurofibroma tissue, keloid and normal skin were collected. The distribution and expression of CTGF was measured by immunohistochemistry. The mRNA expression of CTGF, TGF-β1,ColⅠ and ColⅢ was detected by reverse transcription- polymerase chain reaction. Expression of CTGF protein was measured by western blotting.

收集神经纤维瘤组织、瘢痕疙瘩组织及正常皮肤组织标本，免疫组化检测CTGF的表达，逆转录-聚合酶链式反应方法检测组织CTGF、TGF-β1、Ⅰ型胶原及Ⅲ型胶原 mRNA表达，Western blotting检测组织CTGF蛋白的表达。

It has opened a new way for the diagnosis and therapy of tumours, and also estab- lished the foundation for the carcinogenic mechanism research. In order to study the altered molecular events during laryngeal car- cinogenesis and elucidat the role of oncogenes in laryngeal carci- noma, we examined the Ha-ras and c-myc gene mutation and amplification by the polymerase chain reaction and molecu- lar hybridization technigues respectivly.

癌基因的研究是当前肿瘤领域内的重大课题，正在基础理论和临床医学中进行广泛和深入的研究，癌基因的发现为肿瘤的诊断和治疗开辟了新的途径，同时也为人类开始从分子水平来回答细胞癌变的本质问题，最终认识癌变机制奠定了理论基础。

mdm2 gene expression atmrna and protein levels,p53 protein and eb virus dna were detected by nonradioactive in situ hybridization ,immunohistochemistry and polymerase chain reaction separately in 46 cases of npc tissuesand 12 cases of chronic inflammation of nasopharyngeal epithelium.

运用非同位素原位杂交、免疫组化和多聚酶链反应技术对46例npc、12例慢性鼻咽炎症粘膜分别进行mdm2 mrna、mdm2蛋白、p53蛋白及ebv-dna的检测。

Polymarase Chain Reaction may be the better method of detecting M.tuberculosis than other methods and lead us to carry out a series of research on this problem. This thesis is composed of five parts:(1)systematic evaluation for the first time of the ability of five defferent PCR methods to detect M. tuberculosis;(2) using the endonuclease and southern blot by a radioactive and a nonradioactive labeled probe, to confirm the specificity of the PCR products and to analyse the sensitivity of detection by southern blot method using digoxigenin-labeled probe;(3) evaluation of acid-fast staining, bacterial culture and PCR for the detection of M. tuberculosis in sputum, pleural and peritoneal effusion samples;(4) The feasibility of PCR for detection of M tuberculosis on formalin-fixed paraffin embedded tissues;(5) 26 cases of sarcoidosis were retrospectively studiedwith PCR for M. tuberculosis and nontuberculosis Mycobacterium and the problems on histopatholoical diagnosis of sarcoidosis are discussed.

论文共分为五个部分，(1)首次系统地比较了五种不同引物序列的PCR检测结核杆菌方法的特异性和敏感性等情况；(2)利用限制性内切酶酶切分析和同位素及非同位素标记DNA探针进行Southern杂交的办法，分析PCR产物的序列与原设计是否相符及杂交检测PCR产物对提高检测结核杆菌敏感性的情况；(3)并对PCR用于快速检测临床标本中的结核杆菌进行了初步评价；(4)探讨了PCR用于检测石蜡包埋组织中结核杆菌的作用；(5)并利用PCR检测结核杆菌和非结核分枝杆菌的方法对26例原病理诊断为结节病的病例进行了PCR检测及组织病理学诊断的探讨。

A portable polymerase chain reaction system automates all the steps, including sample preparation, allowing operation by nonscientist users in the field and providing results in only 30 minutes.

一种便携式聚合酶链反应系统能够自动完成包括样品准备在内的所有实验步骤，从而使得不是该领域的非专业操作者也能够完成检测，而且只要30分钟就能够得到结果。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。