查询词典 chain reaction

Aim: To study the feasibility of aneuploidy diagnosis using coelocentesis combining with quantitative fluoresce polymerase chain reaction in early pregnancy.

目的：探讨应用荧光定量PCR法检查早孕期胚胎胚外体腔液胎儿细胞染色体的可行性。

Results The serum antibody level reached to 0.71±0.08-0.77±0.06 after genetic vaccine pCD-HCV1(100 μg/mouse) were inoculated into the mice(n=12) three or four times while blank vector pCD-SRα1 could not induce the mice(n=8) to generate antibody response in same way.After the antibody levels in mice(n=8) immunized by pCD-HCV1 had ascended to peak value(0.71), there was no trend of descending during the following 18 weeks of detection(0.68±0.06-0.75±0.07). Specific fragment of HCV cDNA identified by polymerase chain reaction from DNA extracted from the muscles of the mice after pCD-HCV1 had beed inoculated three months.

结果 基因疫苗pCD-HCV1(100 μg/只)3-4次接种小鼠后(n=12)，血清抗体水平达0.71±0.08-0.77±0.06，空载体pCD-SRα1免疫鼠血清抗体阴性；免疫鼠(n=8)抗体水平达高峰后(0.71)，持续检测18周未见下降趋势(0.68±0.06-0.75±0.07)；pCD-HCV1免疫小鼠3个月后，用聚合酶链反应仍可从肌肉组织中扩增出特异性HCV cDNA片段；免疫鼠PBMC对HCV合成肽CP9、基因重组抗原C、E1刺激后均出现增殖反应，刺激指数分别为4.07±1.58、3.88±0.70和3.69±1.30，与pCD-SRα1免疫鼠相比，差异有显著性(P.001)。

Objective To develop a nested-polymerase chain reaction to identify G and P genotypes of group A rotavirus.

目的用巢式聚合酶链反应对A组轮状病毒常见G、P基因型进行检测。

Gene frequency/genet ; gene amplification/genet ; polymerase chain reaction/methods

基因频率／遗传学；基因扩增／遗传学；聚合酶链反应／方法学

The LV messenger ribonucleic acid levels of contractile and calcium regulatory genes were measured by quantitative real time polymerase chain reaction and normalized for glyceraldehyde 3-phosphate dehydrogenase.

采用定量实时聚合酶链反应测定心室收缩蛋白和钙调蛋白基因信使RNA水平，采用3-磷酸甘油醛脱氢酶标准化。

The rabbits' RNA was extracted from the lavage cells, and mRNA for TNFα was quantitated by reverse-transcription polymerase chain reaction using glyceraldehyde 3-phosphate dehydrogenase as an internal standard.

第二部分：12只表面活性物质缺乏兔，以CMV(n＝6)或PHY+PEEPi(n＝6)通气1h后，重复灌洗，提取灌洗液中的兔RNA，以GAPDH为内参照RT-PCR的方法来定量分析TNFαmRNA。

Methods: Atrial tissue was obtained from the right appendage during open surgery in 25 patients with RHD. The mRNA of ACE and ERK2 and AT1R was semi-qualified by reverse transcription-ploymerase chain reaction and normalized to the glyceraldehyde 3-phosphate dehydrogenase. Western blotting analysis was employed to examine the expression of ACE and ERK and AT1R. Atrial collagen volume fraction was detected by Masson's stain.

25例风心病二尖瓣狭窄接受外科手术者，手术时取右心耳处心房组织500mg，通过逆转录—聚合酶链反应技术，以GAPDH为内参照测量ACE、AT1R、ERK2的 mRNA变化，用Western Blotting了解ACE、AT1R、ERK在蛋白水平表达的差异，经免疫组织化学了解胶原纤维容积分数改变情况。

The authors tested the cervical excretions of 50 women by polymerase chain reaction forgonorrhea and compared the results with those tested by gonococcus cultures.

作者采用聚合酶链式反应诊断女性淋病50例，并将PCR与淋菌分离培养比较，结果50例宫颈分泌物PCR检测获得阳性35例，其中20例与常规分离培养法一致，另外15例PCR阳性而分离培养为阴性，此15例临床诊断为淋病。

Measles virus ; S191; hemagglutinin gene ; reverse transcription polymerase chain reaction ; sequence analysis ; molecular epidemiology

麻疹病毒； S191； H基因；逆转录—聚合酶链反应；序列分析；分子流行病学

A method of one-step reverse transcription polymerase chain reaction was developed to subtype hemagglutinin gene of Avian influenza virus.

建立一步法 RT-PCR 检测方法，对禽流感病毒(Avian influenza virus，AIV)的血凝素(Hemagglutinin，HA)分型进行了研究。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。