查询词典 chain reaction

Methods: A polymerase chain reaction assay with oligonucleotide primers homologous to a portion of the urease C and cytotoxin associated gene A of Hp was used to test the presense of Hp in dental plaque.

依据特异的尿素酶C 基因和 cag A基因设计引物，建立聚合酶链反应方法，检测65例慢性胃病患者不同牙齿的龈上和龈下菌斑中的Hp.6例胃Hp阳性的胃炎患者，经药物治疗后，检测口腔Hp。

HP were determined by rapid urease test,Warthin-Starry silver stain and polyme rase chain reaction,and expression of P21,P53,C-myc and C-erbB-2 were detecte d by immunohistochemicology (Streptaovidin/Peroxidase,SP) method,and were evalua ted with positive expressive integrality,the mean optical density an d the integral optical density in computer assisted image analysis system.

方法经内镜和病理诊断的胃癌91例，用快速尿素酶试验，改良W-S银染色和PCR方法检测上述标本中Hp；以S-P免疫组织化学法检测P21、P5 3、C-mcy及C-erbB-2，并用阳性表达积分半定量和免疫组化图像分析进行比较。

RESULTS: The polymerase chain reaction product was in concordance with target fragment. The analysis of the gene sequence proved it is the ALR gene. The overall length of the ALR gene was 378 bp, it was highly conservative in many species, for instance the Homo sapiens, the Mus musculus, the Bos Taurus and the Danio rerio.

结果：聚合酶链反应扩增结果与目的片段位置一致，测序结果表明为肝再生增强因子mRNA，基因全长378 bp，在人、牛、小鼠、斑马鱼等物种中高度保守。

In order to understand insertion/delation polymorphism of the angiotensin-converting enzyme gene in pilots,and to explore the relationship between ACE gene I/D polymorphism and the perfomance of the pilots,the polymerase chain reaction was used to determine the genotypes for an I/D polymorphism in intron 16 of the ACE gene in 118 pilots and 96 healthy subjects as controls.

为了解飞行员血管紧张素转换酶基因插入或缺失多态性情况，探讨ACE基因多态性与飞行员耐力可能的关系，用聚合酶链反应扩增技术检测118例飞行员和96例健康对照者的ACE基因I/D多态性。

The methods of DNA depuration and detection are proved to be simple,quick and accurate, which establish a good foundation for the assessment of DNA vaccine safety,and have the favourable perspective in application.

荧光定量PCR（Fluorescent Quantative Polymerase Chain Reaction，FQ-PCR技术是美国PE公司研制成功的一种高灵敏度和高精确度核酸检测方法，该法在传统PCR的基础上引入荧光标记探针，从根本上解决了PCR反应中非特异性扩增的难题[4]。

The reverse transcription polymerase chain reaction is a routine diagnostic assay for the detection of hMPV.

RT-PCR技术是hMPV主要的检测手段，hMPV感染也是哮喘急性发作和慢性阻塞性肺病急性加重的诱因。

Methods: Twelve microsatellite markers located at chromosomes 3p,9p and 14q were selected to investigate microsatellite alterations in 31 RCC specimens and their paired metastasis specimens by polymerase chain reaction-polyacrylamide gel electrophoresis-ethylene dibromide (PCR-PAGE-EB) staining and sequencing.

选取位于染色体3p、9p、14q上的共计 12个微卫星多态性标记，采用PCR-中性聚丙烯酰胺凝胶电泳-EB染色和测序的方法对31例肾细胞癌患者的原发病灶和配对的转移病灶进行MSI和LOH分析。

Methods: Twelve microsatellite markers located at chromosomes 3p, 9p and 14q were selected to investigate microsatellite alterations in 31 RCC specimens and their paired metastasis specimens by polymerase chain reaction-polyacrylamide gel electrophoresis ethylene dibromide (PCR-FAGE-EB) staining and sequencing.

选取位于染色体3p、9p、14q上的共计12个微卫星多态性标记，采用PCR-中性聚丙烯酰胺凝胶电泳－EB染色和测序的方法对31例肾细胞癌患者的原发病灶和配对的转移病灶进行MSI和LOH分析。

Both products then serve as DICER substrates, initiating the RdRP chain reaction.

所有的产物又可作为Dicer的底物，起始RdRP级联反应。

Methods We measured messenger RNA levels of Dicer and Drosha in specimens of invasive epithelial ovarian cancer from 111 patients, using a quantitative reverse-transcriptase–polymerase-chain-reaction assay, and compared the results with clinical outcomes.

我们用定量RT-PCR的方法检测了111例侵袭性上皮卵巢癌标本中Dicer 和Drosha酶的mRNA的水平，并且分析其与临床预后的关系。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。