查询词典 centrifugation

After centrifugation, the extracts were separated on HILIC column by using acetonitrile-water as mobile phase. The analytes were identified and quantitated by LC-tandem MS under the positive/negative electrospray ionization and multiple reaction monitormode. The calibration curves showed good linearity in the range of 25-5000 μg/kg for melamine and cyanuric acid, and 50-10000 μg/kg for ammeline and ammelide. The limits of detection and limits of quantification were in the range of 5-20 μg/kg and 50-100 μg/kg, respectively.

牛奶样品直接用乙腈超声波提取；奶粉样品先用水溶解后再加乙腈超声波提取，高速离心后以乙腈－水为流动相，采用亲水作用色谱柱分离这4种化合物，在电喷雾正、负电离切换多反应监测模式下进行定性与定量分析，三聚氰酸一酰胺与三聚氰酸二酰胺含量在50~10000μg/kg之间、三聚氰胺与三聚氰酸在25~5000μg/kg之间，均获得良好的线性。

Methods The models of isolated rat islets were established by collagenase digestion and dextran gradient centrifugation. The influence of various concentrations of amylin on both glucagon and insulin secretion was studied.

应用胶原酶消化和不连续密度梯度离心技术建立离体大鼠胰岛模型，研究不同浓度胰淀素对胰岛素和胰高血糖素分泌的影响。

3Ml blood of antecubital vein from patients with CCF were collected at admission, and 3ml blood of antecubital vein from healthy individuals on an empty stomach in early morning. Added Na2-ethylenediaminetetra-acetic acid to the samples of whole blood, separated plasma by prompt centrifugation of the blood samples at 2500g at 4°C for 10 minutes . The samples were immediately frozen and stored at -80°C.

选择91例心力衰竭患者和26例健康体查者作为研究对象，心力衰竭患者入院后立即抽取肘静脉血液3ml，收集对照者清晨空腹肘静脉血液3ml，EDTA-Na2抗凝，4℃下以2500转/分高速台式离心机离心10分钟后分离血浆，分装保存于-80℃冰箱待测。

METHODS: BMSCs were obtained from 16 fresh adult bone marrow by gradient centrifugation with Ficoll Paque, cultured with DMEM/F12 with 10% umbilical cord blood serum and 10% adult autoserum. The fourth passage was used in this study. The surface antigens of BMSCs were detected by flow cytometry.

采用Ficoll Paque细胞分离液从16例新鲜成人骨髓穿刺液中分离骨髓间充质干细胞，分别用含体积分数为10%脐带血清、成人自体血清的DMEM/F12培养基培养，取第4代细胞进行实验。

Extraction efficiency of sucrose-centrifugation methods which were applied to centrifugate nematodes from soil was studied by means of one-way trial and double-way trial.

采用单因素试验和二因素组合试验，对蔗糖离心法分离线虫的效果进行研究。

By Percoll fluid and density gradient centrifugation,the MSC was obtained;after the cells filled the bottom of vessel,subcultured them,when they subculture in third generation ,redigested them,500 R/min centrifugate,alter the

经percoll液密度梯度离心法能获得纯度较高的人骨髓间充质干细胞，这种细胞在体外培养体系中保持旺盛的增殖能力；经轻度离心和化学限定培养基法可诱导骨髓间充质干细胞向成软骨方向分化，增殖旺盛，可传一代培养，是软骨组织工程理想的种子细胞。

Methods: A 5ml bone marrow was extracted from the lilac of human volunteers. By Percoll fluid and density gradient centrifugation, the MSC was obtained; after the cells filled the bottom of vessel, subcultured them, when they subculture in third generation, redigested them, 500 R/min centrifugate, alter the completed medium to chemical definition medium, examined the form change and prolifration of cells by invert microscope, toluidine blue stain、immunocytochemical stain and RT-PCR to test the type Ⅱ collagen mRNA and proteoglycan.

取健康成人髂后上棘处骨髓5ml，经percoll液分离后密度梯度离心，〓／ml密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心5分钟，〓／ml接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质中的蛋白多糖，免疫细胞化学染色检测ECM中Ⅱ胶原的蛋白合成，RT-PCR鉴定诱导细胞Ⅱ胶原mRNA的表达。

Objective: To examine the property and proliferation ability of primary culturing MSC, investgate the effect of lightly centrifugation and chemical definition medium to induce MSC differentiate into chondroblast.

目的：了解骨髓基质干细胞原代培养的特性及增殖能力，探讨轻度离心和化学限定培养基法诱导骨髓基质干细胞向成软骨方向分化的作用；为软骨组织工程提供足量、合适的种子细胞。

Part one Isolating, culturing, chondroblast induction and evaluation of human MSC in vitroObjective:To examine the property and proliferation ability of primary culturing MSC ,mvestgate the effect of lightly centrifugation and chemical definition medium to induce MSC differentiate into chondroblast.

结果：原代骨髓基质干细胞易于体外培养，增殖旺盛；经轻度离心和化学限定培养基诱导后细胞呈高密度生长，仍保持较旺盛的增殖能力，细胞转化成圆形肥大细胞，甲苯胺蓝染色显示软骨细胞外基质 v的特异性异染，11胶原蛋白免疫细胞化学染色呈强阳性，BT干CR鉴定显示＊胶原mRNA丰富的表达。

Nutrition solution culture, ICP-AES, the differential centrifugation technique and sequential chemical extraction method, suppression subtractive hybridization , RAPD were used in this thesis to study the mechanisms of copper tolerance and accumulation by Commelina communis grown in copper mine deposit which was reported to be a Cu hyperaccumulator, compared with C.

论文以本实验室报道的生长于矿山上的铜超富集植物鸭跖草和生长于正常土壤中的鸭跖草为试验材料，分别运用溶液培养法、ICP-AES元素测定技术、差速离心法和化学试剂逐步提取法、抑制消减杂交技术、RAPD技术研究了矿山和非矿山两种生境鸭跖草对铜的耐性和吸收积累的差异，两种生境鸭跖草在铜胁迫下养分吸收和积累的差异，两种生境鸭跖草在铜胁迫下生理反应的差异，以及铜在鸭跖草细胞内的分布及结合形态研究，矿山鸭跖草差异表达的cDNA克隆，铜对鸭跖草种群遗传分化的影响，从这几个方面探讨了矿山鸭跖草对铜的耐性机理。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。