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Wu39-1,Wu43-1 and Wu50-2 were used to develop a handy extraction method of mtDNA in crucifer s on the basis of the sucrose-bedding method of mtDNA extraction and by means of four combinations of different centrifugal forces,differential centrifugation,and composition adjustment and dosage increase of buffer solution A.

以十字花科植物芸芥 Eruca sativa 、白菜型油菜 Brassica rapa 和甘蓝型油菜 Brassica napus ：武39- 1、武4 3- 1、武5 0 - 2为试材，在蔗糖衬垫法分离纯化m t DNA方法优点的基础上，通过4组不同离心力配比，采用差速离心法、改变缓冲液A的成分以及增加缓冲液的用量，建立了一种简便提取十字花科植物mt DNA方法。

Microcells were produced by centrifugation of micronucleated A9 (neo12) cells in Percoll density gradient containing 20 mg/L Cytochalasin B at 39 000 g. The resulting mixture of microcells, whole cells, karyoplasts and cytoplast fragments was filtered through 8 μm and 5 μm size membrane pores sequentially to obtain pure preparation of microcells.

微核化的细胞在含有20 mg/L细胞松弛B的Percoll密度梯度介质中，经39 000g高速离心后，包含微细胞、完整细胞、细胞核和细胞碎片的混合液，依次通过8 μm和5μm孔径的滤膜过滤后可获得纯化的微细胞溶液。

Microcells were produced by centrifugation of micronucleated A9 (neol2) cells in Percoll density gradient containing 20mg/L Cytochalasin B at 39 000g. The resulting mixture of microcells, whole cells, karyoplasts and cytoplast fragments was filtered through 8 pm and 5 pm size membrane pores sequentially to obtain pure preparation of microcells.

微核化的细胞在含有20mg/L细胞松弛B的Percoll密度梯度介质中，经39000g高速离心后，包含微细胞、完整细胞、细胞核和细胞碎片的混合液，依次通过8μm和5μm孔径的滤膜过滤后可获得纯化的微细胞溶液。

Virgin olive oils are the oils obtained from the fruit of the olive tree solely by mechanical or other physical means under conditions, particularly thermal conditions, that do not lead to alterations in the oil, and which have not undergone any treatment other than washing, decantation, centrifugation and filtration.

原始橄榄油是油从果实的橄榄树只用机械或其他物理手段的情况下，特别是热条件下，不会导致改建的油，并没有经过任何治疗以外洗衣机，迁，离心，过滤。

One could denature the DNA, and then subject the single-stranded sample to CsCl centrifugation.

CsCl梯度离心的结果虽然排除了全保留复制的可能性，但不能根据这一结果就可证明DNA的复制是半保留复制，因为弥散复制也会出现同样的结果。

By releasing the cytosol proteins with Digitonin and centrifugation technology, we divided the J6-2 cell proteins into the cytosol proteins and the particulate-conjugated proteins, and then using SDS-PAGE, Western blotting and Enzyme-linked immunosorbent stain combined with computer quantiscan technology, we investigated the effect of GM3 on PLC1 translocation.

本文采用洋地黄皂甙增加细胞膜渗透性，释放胞液蛋白，通过离心将细胞蛋白分为胞液蛋白和颗粒结合蛋白两部分，然后采用SDS-PAGE，Western blot及酶联免疫显色技术，并结合计算机扫描半定量技术，检测了GM3处理前后PLC1在细胞内的转位情况。

The conventional rock electricity experiment get the irreducible water saturation with centrifugation,we think it is higher than its actual value.At present,we think the irreducible water saturation that get from mercury intrusion method is accurate.In view of the disadvantages of mercury intrusion method,we analysis the effect of bark of bluish dogbane and correct the experiment data,finally get reasonable data.

常规的岩电实验用离心法得到的束缚水饱和度普遍以为其偏高，目前以压汞法得到的束缚水饱和度为准，本文考虑压汞实验的弊端，深进分析"麻皮效应"对计量结果的影响，对压汞实验数据进行校正，得到了较为公道的实验数据。

Results The purity as well as removal rates of egg albumin and foreign protein of virus bulk purified by Sepharose 4FF gel chromatography were slightly higher than those by sucrose density gradient centrifugation.

结果 层析法的纯度、卵清蛋白及杂蛋白去除率略高于蔗糖密度梯度离心法，而蔗糖密度梯度离心法的病毒回收率优于层析法。

OVCs were isolated by density ladder centrifugation in the 4th week, and then OVC's morphology was observed under transmission electromicroscrope and immunocytochemistry were performed to detect the expression of ICAM-1. Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast.

结果 透射电镜下观察到OVCs超微结构的改变为核大，核/浆比大，核仁小，胞浆内细胞器少，可见少量内质网和小线粒体，整个细胞呈幼稚、未分化状态；ICAM-1免疫细胞化学检测阳性；损伤肝组织HE染色和ICAM-1免疫组化检测显示，肝损伤初期，Hering管周围OVCs增生并且ICAM-1阳性表达，随着肝损伤的加重，OVCs继续增生，并且，OVCs沿增生的肝纤维向肝小叶迁移，同时，ICAM-1阳性表达也继续增多，并逐渐向肝小叶弥散分布；透射电镜下观察到，肝损伤初期，OVCs在Hering管周围增生，肝纤维随之增生，增生的OVCs与肝纤维黏附生长。

OVCs were isolated by density ladder centrifugation in the 4th week, and then OVC's morphology was observed under transmission electromicroscrope and immunocytochemistry were performed to detect the expression of ICAM-1. Results Observing OVC's morphology under transmission electromicroscrope showed that OVC was infantile and undifferentiated with big nucleus, clear nucleolus, large nucleoplasm-ratio, small mitochondria, and little endoplast. In initial stage of damaged liver, OVCs and the expression of ICAM-1 mostly distributed around Hering duct, and then gradually increased and expanded toward hepatic lobule, shown by staining paraffin sections with HE, immunochemistry and transmission electromicroscrope. Immunocytochemistry indicated that ICAM-1 expression on OVCs was positive.

结果 透射电镜下观察到OVCs超微结构的改变为核大，核/浆比大，核仁小，胞浆内细胞器少，可见少量内质网和小线粒体，整个细胞呈幼稚、未分化状态；ICAM-1免疫细胞化学检测阳性；损伤肝组织HE染色和ICAM-1免疫组化检测显示，肝损伤初期，Hering管周围OVCs增生并且ICAM-1阳性表达，随着肝损伤的加重，OVCs继续增生，并且，OVCs沿增生的肝纤维向肝小叶迁移，同时，ICAM-1阳性表达也继续增多，并逐渐向肝小叶弥散分布；透射电镜下观察到，肝损伤初期，OVCs在Hering管周围增生，肝纤维随之增生，增生的OVCs与肝纤维黏附生长。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。