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Second, procedures of effective protoplasts fusion and U.V. irradiation or heat treatment protoplasts fusion were developed. Equal protoplast suspensions (containing about 108/ml protoplasts) of each strain were mixed and washed by centrifugation, then the pellets were resuspended in PEG4000 solution (35%) at 37癈 for 2min.

其次，确定了原生质体融合的基本条件及灭活原生质体融合的条件：等量混合两亲株原生质体或用热灭活的原生质体(＞10~8个／ml)，用35％的PEG4000在37℃处理2min后，稀释，涂布再生，可获得融合率在50％左右的融合子。

Methods: Hepatic stellate cells were derived from normal rats by in situ perfusion, digestion and density gradient centrifugation with 108 g/L Nycodenz. The cells were stimulated by various concentrations of PDGF or MDA, and then treated with SAB.

采用原位灌注法消化大鼠肝脏，108g/L Nycodenz密度梯度离心，分离HSC，以MTT法观察细胞的增殖能力，免疫组化法检测血小板生长因子受体含量。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。