查询词典 centrifugation

Aureus fermentation media, the centrifugation pelleting the bacterial cells and enriching the media with the secreted Protein A.

也可以通过金黄色葡萄球菌发酵培养基的连续高速离心消除葡萄球菌细胞，离心使细菌细胞沉淀且使培养基富含分泌的蛋白A。

The result indicated that after the yellow seedlings of wheat was triturated by the high speed tissue triturating machine, high purity mitochondria could be exacted by density gradient centrifugation, and then the mitochondria was ruptured by the 5% SDS and protease K, and the protein was wiped off by organic liquid, pure mtDNA could be extracted.

结果表明，经高速组织匀浆捣碎机研磨小麦黄花苗，简易的密度梯度离心技术快速抽提大黄的高纯度的线粒体，5% SDS和蛋白酶K裂解线粒体及有机溶剂抽提去除蛋白质可获得纯度较高的mtDNA。

The results showed that plenty of the purified DPV virions were obtained after the cell lysate was condensed by 5-fold by ultrafiltration,and the concentrated DPV fluid was ultracentrifuged with 300g/L sucrose cushion method and 300-600g/kg sucrose density gradient centrifugation methods.

结果显示，用切向超滤法将病毒培养物浓缩为原体积的1/5后，再采用300g/L蔗糖垫底超速离心和300～600g/kg蔗糖密度梯度离心的方法进行纯化，可获得大量的纯化病毒粒子。

The liner-forming technologies in solid rocket motor such as centrifugation molding,brush coating,pull coating,spray coating,and so on have been developed continuously at home.

我国固体火箭发动机衬层成型工艺如离心成型、刷涂、拉涂、喷涂等工艺不断向前发展。

Results obtained included:(1) MSCs began to attach to culture flask after 24h cell inoculation by centrifugation method.

5观察经Ⅰ型胶原包被的多孔块型PLGA材料体内成骨效果，尤其是材料中心部位的成骨情况。

Methods: Isolated and cultured human EPCs from bone marrow sample with Ficoll gradient centrifugation and differential adhesion culture in sequence, meanwhile, built a new pulsatile bioreactor with T-PLS pump system and a modified air-liquid exchange passage.

通过Ficoll密度梯度离心从人骨髓标本中获得骨髓单个核细胞，差异贴壁法从中分离培养内皮祖细胞。

The following results were obtained.(1) The dissociation of some LHCⅡs from PSⅡ complex occurred after saturating illumination, but FSBA treatment inhibited the dissociation as shown by analysis of sucrose density gradient centrifugation and low-temperature (77K) chlorophyll fluorescence.(2) A significant increase in Fo and decrease in Fv/Fm occurred after saturating illumination, and the two parameters could largely recover to the levels of dark-adapted leaves after subsequent 3 h in the dark, but they could not recover when FSBA existed during saturating illumination.

获得如下结果：（1）饱和光照明可以引起部分LHCⅡ从PSⅡ核心复合体上脱离，但FSBA处理可以抑制LHCⅡ脱离；（2）大豆叶片经过3小时饱和光处理后，初始荧光Fo明显升高，而PSⅡ光化学效率Fv／Fm显著降低，但经过随后3小时暗恢复后，Fo和Fv／Fm基本可以恢复到初始暗对照的水平，而FSBA处理增大它们的变化程度，并且放置暗中3小时后不能恢复。

Concrete formula adopted computer control sand, stone, water, cement, admixtures measurement as well as various specifications of models with a variety of processes ( centrifugation, suspended roller, plug-in, PCCP, wind-driven, core-mode vibration technology) series die, various interfaces, such as drainage pipes: level I, level I, socket type, F-type pipe jacking mode, three-stage pressure pipe vibration Process modules for the largest caliber DN3m 4m (domestic exclusive use of stress to eliminate mold, sand blasting processes), is China's only capable of producing PCCP steel cylindrical pressure tube and a variety of cement pipes, such as a full set of equipment and tooling manufacturers, who national key project ¤- Inner Mongolia Wanlian Brothers Pipeline Company Diversion Project, Jilin pipeline, dragon pipe industry, Fujian Nampo, Xi'an, Shanghai tunnel company, REN Bang Tianjin, Beijing, Hubei, China and South Africa, Zhengzhou Jinlong, Nanjing, Zhejiang Tenglong Xinjiang National Unification, Liaoyang Xiaotun so water and drainage pipes around the country supporting the project.

混凝土配方采用电脑程序控制砂、石、水、水泥、外加剂计量以及各种规格型号与各种工艺的（离心、悬辊、插入式、PCCP，风动、芯模振动工艺）系列模具，各种接口形式，如排水管：平口、企口、承插式、F型顶管模、三阶段压力管振动工艺模的最大口径为DN3m×4m（国内独家采用模具应力消除、喷砂工艺生产），是全国唯一能生产PCCP钢筒压力管以及各种水泥管等全套设备与模具的厂家，曾为国家重点工程─—内蒙古万联兄弟管道公司引水工程、吉林管道、巨龙管业、福建南浦、西安、上海遂道公司、天津任邦、北京、湖北中南、郑州金龙、南京、浙江腾龙、新疆国统、辽阳小屯等等全国各地引水与排水管工程配套。

METHODS: Umbilical cord blood samples were sterilely isolated using Percoll density gradient centrifugation to harvest intermediate layer cells. DMEM medium containing fetal bovine serum, penicillin, streptomycin and L-glutamine was added. Following several adherences and purification, the floating cells were discarded. Thus, many adherent cells with a confluence were collected. When cells were 60%-80% confluent, cells were digested by trypsin for subculture.

无菌条件下，应用Percoll密度梯度离心法分离脐血标本，收获中间层细胞，加入含胎牛血清、青霉素和链霉素、L-谷氨酰胺的DMEM基础培养液，再经反复贴壁纯化，除去悬浮生长细胞，得到较多呈融合状态的贴壁细胞，待细胞达60%~80%融合时胰酶消化传代。

By using centrifugation to impurity elimination, we determined the dosage of supplemental material in the formulation of the tablets as follows: 1 time calcium carbonate, dextrin 10%, microcrystalline celluloses 0.5%, and magnesium stearate 0.3%.

最佳提取工艺为药材加10倍量的水，浸泡1.0 h，回流提取2次，每次1.0 h，采用离心法除去杂质，每个处方的辅料用量为：1倍量的碳酸钙，质量分数为10%的糊精、0.5%的微晶纤维素和0.3%的硬脂酸镁。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。