查询词典 centrifugation

Finally, high-speed centrifugation was applied to the conservation of crude phycobiliprotein, indicating,its effects on removement of denaturalized protein molecule with loss of phycobiliprotein.

最后研究了高速离心对脱盐液保存的影响，认为其在沉降大分子物质和变性蛋白的同时，也必然会使目标蛋白有所损失。

Finally, high-speed centrifugation was applied to the conservation of crude phycobiliprotein, indicating,its effects on removement of denaturalized protein molecule with loss of phycobiliprotein.

最后研究了高速离心对脱盐液保存的影响，认为其在沉降分子物质和变性蛋白的同时，也必然会使目标蛋白有所损失。

Collect Agrobacterium cells by centrifugation at 4,000g for 10 min at room temperature, and gently resuspend cells in 1 volume of freshly made 5% sucrose solution with a stirring bar.

在 4，000g 的离心分离的 8|收集 Agrobacterium 细胞 10 分钟在室温，而且逐渐地以一根活泼的酒吧新做的 5%蔗糖解决在 1 册中再中止细胞。

Chinese scientists have developed a method to separate mixtures of metallic and semiconducting single-walled carbon nanotubes by a two-step dispersion-centrifugation process.

我国科学家已经开发出一种方法，独立的混合金属和半导体单壁碳纳米管由两步分散离心过程。

METHODS: Bone marrow was sterilely isolated. BMSCs were isolated and purified by the density gradient centrifugation.

无菌条件下抽取猪骨髓液，密度梯度离心法分离纯化骨髓间充质干细胞。

METHODS: Mouse bilateral femur was sterilely isolated. BMSCs were harvested by the Percoll density gradient centrifugation, and purified and proliferated by the adherent method.

无菌分离小鼠双侧股骨，Percoll密度梯度离心法分离骨髓间充质干细胞，再用贴壁筛选法纯化扩增。

On basis of mention , we investigate the ability of isolation, purification and expansion of adult rat mesenchymal stem cells to nerve cells in vitro.MethodsBone marrow mononuclear cells from adult rat femur and tibia were obtained sterilely by Percoll ( 1. 073 103 g/L ) gradient centrifugation .

根据MSCs与其它细胞的密度不同而采用Percoll(1.073×10~3g／L)分离液将其分离，此方法分离MSCs所获得的细胞种类较单一，Percoll分离液将骨髓细胞分为三层，取含MSCs的低密度层进行培养。

METHODS: Bone marrow was sterilely separated from human. After heparinization, human BMSCs were harvested using density gradient centrifugation and adherence method. At the fifth passage, BMSCs at 1×108/L were incubated in the 6-well plate and divided into 2 groups. BMSCs in the edaravone group were 50% confluent and incubated in L-DMEM containing basic fibroblast growth factor and fetal bovine serum for 24 hours. After washing in PBS, these BMSCs were incubated in serum-free L-DMEM containing 20 mg/L edaravone for 24 hours. BMSCs in the blank control group were incubated in L-DMEM, supplemented with 10% fetal bovine serum.

无菌抽取的骨髓经肝素化后，采用密度梯度离心法及贴壁筛选法分离获得人骨髓间充质干细胞，传至第5代按1× 108 L-1接种于6孔板内，设立2组，依达拉奉组细胞达50%融合时用含碱性成纤维生长因子、胎牛血清的L-DMEM预诱导24 h，PBS洗涤后再用20 mg/L依达拉奉无血清L-DMEM诱导24 h；空白对照组始终用含体积分数为10%胎牛血清的L-DMEM培养，不加任何预诱导剂和诱导剂。

METHODS: Rat double lower limbs were isolated sterilely. Bone marrow was obtained by rinsing using low-glucose DMEM. BMSCs were isolated and purified by the whole bone marrow adherence method and density gradient centrifugation.

无菌条件下分离大鼠双下肢，低糖DMEM培养液冲出骨髓，分别运用全骨髓贴壁法和密度梯度离心法体外分离骨髓间充质干细胞，利用差速贴壁原理对细胞进行纯化扩增。

METHODS: Bilateral testis of Kunming male mice aged 5-7 days were sterilely collected. Spermatogonial stem cells were isolated and purified using adherence and noncontinuity Percoll density gradient centrifugation.

无菌收集5~7 d龄昆明雄性小鼠双侧睾丸，采用差速贴壁联合非连续性Percoll密度梯度离心法分离纯化精原干细胞。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。