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The apoptosis of 80 mg/kg ﹒ d group was mainly located in spermatogenous cell and first spermatocyte; the apoptosis of 120 mg/kg ﹒ d group was mainly located in spermatogenous cell , first spermatocyte and spermatocyte of the second order; the apoptosis of 240 mg/kg ﹒ d group was mainly located in spermatogenous cell and first spermatocytes, and seemingly, the apoptosis located in first spermatocyte was dominant; whereas the apoptosis of 480 mg/kg ﹒ d group was located in all levels spermatogenic cells.

结果表明：各试验组小鼠生精细胞凋亡指数随着浓度的升高有增加的趋势，而且与对照组差异显著（ P ＜0.05或 P ＜0.01）。80 mg/kg · d 组凋亡细胞主要定位于精原细胞和初级精母细胞；120 mg/kg · d 组凋亡细胞主要定位于精原细胞、初级精母细胞和次级精母细胞；240 mg/kg · d 组凋亡细胞主要定位于精原细胞、初级精母细胞，以初级精母细胞为最多；而480 mg/kg · d 组凋亡细胞定位于各级生精细胞。

Meanwhile,many researches have indicated that ischemia,oxygen deficiengcy and excessive sound stimulus all induce inner hair cell and supporting cell to release excess glutamate which poison the hair cell, afferent neurofibers,ganglion spirale.

同时，许多研究表明，耳蜗缺血、缺氧或过度声刺激均可使耳蜗内毛细胞、支持细胞释放大量Glu，对耳蜗内毛细胞、外毛细胞、传入神经纤维和螺旋神经节产生毒害作用。

The protein had the same antigenicity as IgG, and the change of the protein had the same tendency as SHP-2, which was higher in canceration groups than in uncanceration groups and control groups in thymus cells, but there weren't significant change in bone marrow cells;⑧ The change law of the expression of SHP-1 and SHP-2 during γ-ray induced malignant transformation in BEAS-2B cell is that the expression of SHP-1 was increased synchronistically with malignant transformation of cell, and the expression of SHP-2 didn't have significant correlation with malignant transformation of cell.

癌变组胸腺细胞中SHP-2 mRNA编码PTPase催化区的700～1096bp间可能出现甲基化；⑦癌变组的胸腺细胞中存在一相对分子质量约为55×10〓的蛋白质，它与IgG具有相同的抗原性，其表达量的变化趋势与SHP-2的蛋白表达变化趋势基本一致，即在胸腺细胞中明显高于对照组及未癌变组，但在骨髓细胞中对照组高于癌变组；⑧SHP-1、SHP-2在γ射线诱发的BEAS-2B细胞恶性转化过程的变化规律：SHP-1蛋白表达量的升高与细胞的恶性转化在时间上基本一致，而SHP-2蛋白表达量与恶性转化无明显关系。

Establishment of the murine tumor cell lines expressing human M-CSFR-m-M-CSF fusion gene: The syngenic BALB/c mouse myeloma derived cell line SP2/0 was transfected with pF. Four stable expression cell lines were obtained after selection using G418 and cloning by limiting dilution.

表达人M-CSFR-m-M-CSF的小鼠肿瘤细胞系的建立：以pF转染小鼠骨髓瘤细胞系SP2/0，应用G418筛选，经克隆化培养建立了四株稳定表达人M-CSFR-m-M-CSF的SP2/0细胞系。

Animals, as material, were used to study the toxicity of outer substance and made sure the estrogen effect of BBP by whole animal experiment, and the chronic toxicity of BBP was studied, designed and valued through several dosages and different exposed time. The result showed that BBP could make testis, epididymis and prostate withdrawl. Tectology changes were observed in testis, epididymis and liver. Especially the atrophy and degeneration of seminiferous tubules, decrease of spermatogenic cell and the turbulence of Sertoli cell and spermatogenic cell of seminiferous epithelium were also observed. The linkage between cells vanished.

明确了BBP的雌激素效应，并分别用多剂量和不同染毒时间来研究、设计、评价BBP在体内的慢性毒性，结果表明：BBP能引起雄鼠睾丸、前列腺、附睾性腺的萎缩和肝脏肿大，改变睾丸、前列腺、肝脏的组织形态学，尤其引起睾丸曲细精管萎缩、变性，各级生精细胞减少，生精上皮内生精细胞和Sertoli细胞结构明显紊乱，细胞间连接结构消失等。

Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection. A signal significantly greater than background binding was observed from the third to the fifth round of selection on CHOEGFRGFP1 and CHOK1 cell, but a part of polyclonal scFv bound EGFR specially. About 45% of the selected clones contained a fullsized insert of 1 kb. One unique human antiEGFR scFv (F4scFv) was isolated by analyzing with cell ELISA and DNA sequencing.

结果 经过5轮筛选，细胞裂解液中洗脱出噬菌体效价有500倍以上增长；细胞ELISA结果显示多克隆pscFv与CHOEGFRGFP1细胞和CHOK1细胞均有明显结合，但有部分特异性结合EGFR；菌落PCR显示约45％克隆中含有完整的1kb scFv片断；经细胞ELISA、 DNA测序检测共获得1株EGFR特异性单链抗体，命名为F4scFv。

Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

It was expressed on trophoblastic cell, interstitial cell and endothelium cell of interstitial blood vessels of villi.

结果 ①所有的标本绒毛滋养层细胞、绒毛间质细胞、毛细血管内皮细胞都有HAV的存在。

Measure normal early pregnancy choria tissue and JAR cell by RT-PCR, LH/hCG receptor mRNA expression on both pregnancy choria tissue and JAR cell which is consistent with that be measured in ovarian granulosa cells, indicate that JAR cell and normal choria trophocyte presence LH/hCG receptor, hCG is one of the autocrine hormone.

二、用RT-PCR法可测到正常妊娠早孕绒毛组织、JAR细胞的LH／hCG受体mRNA表达，与正常卵巢颗粒细胞所表达的LH／hCG受体mRNA一致，提示JAR细胞与正常绒毛滋养细胞存在LH／hCG受体，hCG为其自分泌激素之一。

Trypan blue exclusion was used to measure the effect of TCS on cell growth and flow cytometry was used to detect the effects of TCS on cell apoptosis and cell cycle.

用台盼篮染色法检测TCS对细胞生长的影响，应用流式细胞术检测TCS诱导细胞凋亡的情况及对细胞生长周期的影响。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。