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The results showed that ACP positive reactions were mainly deposited in lysosome, nucellar cell, cellular endomembrane, mitochondriurn, and endoplasmic reticulum in four tissues of the healthy shrimp. Furthermore, ACP activity was located in microvilli, and around partly lipid droplet in liver. AKP positive reactions were mainly deposited in cell membrane, nucellar cell, lysosome. endoplasmic reticulum and cellular endomembrane in four tissues.

结果显示，在健康的对虾体内，ACP依次出现在各组织中的溶酶体、细胞核、细胞内膜、线粒体、内质网以及肝脏组织中的部分脂滴周围及微绒毛中AKP依次出现在各组织中的细胞膜、细胞核、溶酶体、内质网以及肝脏组织中的脂滴周围及微绒毛中。

The level of β-tp was in direct proportional to the proliferation of cell in the oligodendroglioma cell lines (SHG-44, U-251, BT-325), but not in the non-oligodendroglioma cell lines (oophroma, prostate-carcinoma and neuro-oocyte).

但在非胶质瘤细胞株，如卵巢癌细胞，神经母细胞瘤细胞及前列腺癌细胞株等，β-tp的浓度与细胞增殖的趋势相反。

The result of this study indicated that the optimum growth condition of GS-101 on agar medium are at 26℃ in pH 5, and the carbon and nitrogen sources are fructose, and corn steep liquor. Through the experiment of shaking flask, the starch was approved to improve the biomass of the culture. However, without starch the fermented broth oppositively has the activity of inhibiting fibrosarcoma cell (HT-1080) and breast cancer cell (MDA-MB-231) cell.

研究结果显示，GS-101使用洋菜培养基探讨较适生长条件为温度26℃，pH 5，碳源为fructose，氮源为corn steep liquor；从摇瓶试验得知starch虽能增加菌丝乾重，但没添加starch的发酵液具有抑制纤维肉瘤细胞(HT-1080)和乳癌细胞(MDA-MB-231)的活性。

Adding fresh medium to cultures, when they had been cultured for 5-8 days, could promote cell sustained division, after which, adding a medium with lower concentration osmatic substance or without osmatic one could futher stimulate the cell division and ensure the active growth of the cell masses.

采用液体浅层培养方法，对埃斯基和雷蒙特红豆草的原生质体进行了培养。埃斯基花茎愈伤组织的原生质体在VK和Kao＇s培养基中2-6天分裂，2周内形成细胞团，19天时的分裂率和克隆率分别为71.41-76.19％、64.78-67.03％。

This experiment is for the purpose of unifying the porine ovocyte in vitro mature raise and the ROSI correlation operation, establishes in the ovicell nature the round sperm cell microinjection fertilization technology procedure, the exploration porine round sperm cell micro fertilization possibility, the research affects this kind of special cell microinjection fertilization effect the factor, for further studies the porine ROSI technology to provide the basic data.

本试验旨在结合猪卵母细胞体外成熟培养及ROSI相关操作，建立卵胞质内圆形精细胞显微注射受精技术程序，探索猪圆形精细胞显微受精的可能性，研究影响这种特殊细胞显微注射受精效果的因素，为进一步研究猪ROSI技术提供基础资料。

The list of possible causes of diabetes insipidus in this case and many others is extensive and includes infection (tuberculous pachymeningitis and Whipple's disease), malignant neoplasms (germ-cell tumor and lymphoma), infiltrative processes (hemochromatosis and amyloidosis), autoimmune diseases (Wegener's granulomatosis, lymphocytic infundibulitis, and sarcoidosis), and histiocytosis (Langerhans'-cell histiocytosis and non-Langerhans'-cell histiocytosis).

这例病例其他许多病例尿崩症的可能病因广泛，包括感染(结核性脑脊髓膜炎和Whipple病)，恶性肿瘤，变性疾病，自身免疫病（Wegener肉芽肿，垂体茎淋巴细胞瘤和粒细胞肉瘤）和组织细胞增生症（Langerhans细胞和非Langerhans细胞组织细胞增生症

Methods DCs were pre pared from peripherad blood mononuclear induce d with gra-nulocyt e／macrophage colony-stimulating factor and interleukin-4.Apoptosis of he patochlangioma cells were induced with γ-radiation.We design these experiment groups including(1)coculture of DCs and apoptotic cancer cells and T cells,(2)co culture of DCs and necrosis cancer cells and T cells,(3)coculture of DCs and cul tured cancer cell and T cells,(4)cocultu re of DCs and T cell,(5)cocuture of DCs and cultured cancer cell.

用粒-巨噬细胞集落刺激因子加白介素-4(IL-4)从人外周血分化、诱导DCs、γ-射线在体外诱导培养的人胆管癌细胞凋亡，将DCs、T淋巴细胞和凋亡胆管癌细胞共培养，同时设计不同类型肿瘤细胞(坏死胆管癌细胞及培养胆管癌细胞)作对照，7d后，分离、富集DCs、T 淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。

Coli. Transmission electron microscopy was used to evaluate the cell morphology of both the normal and the treated E. coli. The observation with TEM suggested that silver nanoparticles lead to the formation of "pits" in cell wall of the bacteria, and silver nanoparticles could enter into periplasm through the pits and destroyed the cell membrane.

采用透射电镜观察了经纳米银粒子处理过的大肠杆菌细胞形态变化过程，结果显示纳米银粒子先在细胞壁上产生小的孔洞，通过这些孔洞进入周质空间，导致细胞膜成分渗漏和破坏细胞膜，进而进入细胞内部。

We generated EG4-GFP cell lines expressing enhancing green fluorescence protein which still maintained typical characteristics of pluripotent stem cells. We microinjected green cells into perivitelline of 8-cell stage embryos as well as aggregated them to 8-cell stage embryos with zona pellucida removed, to investigate developmental fate of EG4-GFP cells during formation of chimeric blastocysts.

我们建立了稳定表达绿色荧光蛋白的EG细胞系（EG4-GFP），发现该细胞仍能保持野生型的一般生长特性；通过显微注射和体外聚集得到细胞与8-细胞受体胚胎共同发育形成的嵌合胚，发现转基因细胞参与了胚胎早期发育过程中的生长与分化，大部分细胞集中于内细胞团，也有细胞分化为滋胚层细胞。

Affect on the liver pathological state after XIEZHIPING treated: the golden hamster hepatic tissue morphous structure of normal control was normal, which had regular hepatic lobules and no lipid droplet;that of model control manifested diffuse heavy adipose degeneration and heavy ballooning degeneration with inflammatory cell infiltrate which mainly composed of lympholeukocyte;that of high dose group showed midrange ballooning degeneration with chronic inflammatory cell infiltrate which mainly composed of lympholeukocyte and plasmocyte;that of media dose group indicated slight focus ballooning degeneration with a small quantity normal hepatic cells in some locals;that of low dose group appeared midrange adipose degeneration with a few lympholeukocyte cell infiltrate in the parts of central veins and port area.

泻脂平治疗后对肝脏病理状况的影响：空白对照组金黄地鼠的肝组织形态结构正常，肝小叶规则，无脂滴发现；模型对照组肝脏弥漫性重度脂肪变性，发生重度气球样变，伴见以淋巴细胞为主的慢性炎性细胞浸润；泻脂平高剂量组肝细胞呈现中度气球样变，伴见以淋巴细胞、浆细胞为主的慢性炎性细胞浸润；中剂量组肝细胞轻度灶性气球样变，局部可见少量正常肝细胞；低剂量组肝细胞呈中度脂肪变性，可见中央静脉及汇管区些许淋巴细胞浸润。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。