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Results There were more mineralization nodes to form in shorter time in the 1×105/ml cell density group compared with in the 2×105/ml cell density group, but in the 5×105/ml cell density group, there were not, and with the DMEM medium contained β-glycerophosphate sodium and ascorbic acid, mineralization nodes formed more early compared with the DMEM medium.

结果 在培养30天内，条件培养液或常规培养液培养的5×105个/ml组细胞无结节形成；而2×105个/ml组和1×105个/ml组都有钙化结节形成，而且有细胞越少形成结节越早，数量越的趋势；给予条件培养液又较常规培养液培养有结节形成早而多的趋势。

Results There were more mineralization nodes to form in shorter time in the 1×105/ml cell density group cnpared with in the 2×105/ml cell density group, but in the 5×105/ml cell density group, there were not, and with the DMEM medium contained β-glycerophosphate sodium and ascorbic acid, mineralization nodes formed more early cnpared with the DMEM medium.

结果 在培养30天内，条件培养液或常规培养液培养的5×105个/ml组细胞无结节形成；而2×105个/ml组和1×105个/ml组都有钙化结节形成，而且有细胞越少形成结节越早，数量越多的趋势；给予条件培养液又较常规培养液培养有结节形成早而多的趋势。

Results There were more mineralization nodes to form in shorter time in the 1×105/ml cell density group compared with in the 2×105/ml cell density group, but in the 5×105/ml cell density group, there were not, and with the DMEM medium contained β-glycerophosphate sodium and ascorbic acid, mineralization nodes formed more early compared with the DMEM medium.

结果 在培养30天内，条件培养液或常规培养液培养的5×105个/ml组细胞无结节形成；而2×105个/ml组和1×105个/ml组都有钙化结节形成，而且有细胞越少形成结节越早，数量越多的趋势；给予条件培养液又较常规培养液培养有结节形成早而多的趋势。

To effectiely study the effect of these heterotypic cell-to-cell interactions, it is necessary to establish a culture enironment that controls the interactions between multiple cell types.

为了有效地研究这些异型细胞之间的相互作用，建立一个能控制多种细胞间相互作用的环境是非常必要的。

To further investigate their roles in cell proliferation apoptosis and differentiation, three human leukemia cell lines (K562, U937 and HL60) exposed to various concentration of hexamethylene bisacetamide, a potent inducer of differentiation of transformed cells both in vivo and in vitro, were used as an in vitro model for the study of cell proliferation, differentiation and apoptosis.

为了进一步研究cyclin A和p21在白血病细胞增殖，分化，凋亡中的作用，我们用在体内，外均有抗肿瘤作用的药物六亚甲基二乙酰胺在体外抑制白血病细胞株K562，U937和HL60增殖，诱导其分化，成功地建立了与药物干预浓度呈剂量-效应关系的，具有不同增殖，分化，凋亡状况的实验细胞模型。

Restrictive changes of TCR Vp repertoire and clonal expanded T cells could be found in peripheral blood T cells from patients with T-ALL. However, the clonal expanded T cells' property (clonal expansion leukemic cells or leukemia antigen-specific expansion T cells) should be further characterized. It may play a certain role on detection of minimal residual disease and design of anti-leukemia idiotypic vaccine. The dominant utilization of TCR VjJ repertoire could be found in peripheral blood T cells from patients with B-ALL, meanwhile clonal expansive T cells were existed. It may be a feature of the host immune response for leukemia-associated antigen. The clonal expansive tendency of T cells in V021 and VP23 subfamilies was rather obvious, which may be correlated with certain malignant B-cell clone. The CDR3 sequences of monoclonal expansive T cell in T cell strains were different, which was the base of developing DNA vaccine.

T-ALL患者外周血T细胞的TCR Vβ谱系出现限制性改变，均可检测到克隆性增殖T细胞，尚需进一步鉴定其性质(肿瘤性或抗原特异性增殖)，对于研究微小残留病变检测和设计抗白血病独特型疫苗均有一定的意义。B-ALL病人外周血T细胞的TCR Vβ谱系呈现优势利用的特点，并存在克隆性增殖的T细胞，这可能是机体对白血病相关抗原产生的特异性免疫反应；Vβ21和Vβ23亚家族T细胞发生克隆增殖改变的趋向性比较明显，可能与B-ALL相关抗原刺激有关。T细胞株中检测到的肿瘤克隆其CDR3序列具有高度的特异性，是构建DNA疫苗的基础。

Increase the bone marrow cell component while reduce the fat component, abnormal the caryon and cytomembrane structure of bone marrow cell and stromal cell, enrich the ribose body, mitochondrion and rough surfaced endoplasmic reticulum in cytoplasm of several organs and tissues, embody abvious improvement of heart muscle, liver, immnune organs, incretory organs and sexual gland.

增加免疫介导型再障小鼠的骨髓细胞成分，减少脂肪成分。使骨髓细胞和基质细胞的细胞核和细胞膜结构趋于正常，使多种脏器组织的细胞浆内核糖体丰富，线粒体及粗面内质网发达。对心、肝、免疫器官、内分泌器官及性腺等亦有明显的改善作用。

Results Established prostate cancer cell lines are eventually infectible by adenoviral vector. The ratio of vector/cell at which infection occurs depends on the specific cell line.

结果腺病毒载体可以转染所有已建立的前列腺癌细胞株，但是每种细胞株转染所要求的载体浓度以及暴露时间不同。

ResultsIn group C and D hyperplasia of epithelium cell and infiltrations of inflammative cell in day 14 that were severe than that of day 7,ciliate cell was piece loss,the glands were hyperplasia（≥40% of group C,≤20% of group D）in windpipe,and pulmonary alveolus were congestion and dropsy.In group C the epidermis were pucked and connective tissue was hyperplasia in the skin,the quantity of hair ball and the sweat glands of group C and D in the food were significantly reduced than that of group A.

结果第14天时，C组、D组气管上皮鳞状化生，C组气管纤毛多呈片状缺损，而D组多呈灶状缺损；两组气管黏膜炎性细胞浸润均较第7天为重，伴气管浆液腺上皮黏液腺化生（D组≥40%、C组≤20%）、肺泡淤血、水肿。C组皮肤表皮呈不规则皱缩、毛球数明显减少；C组、D组足垫部皮肤表皮增厚、汗腺减少伴结缔组织增生。

Spontaneous germ cell death is very important for the removal of germ cells and control of the quantity and quality of sperm. But excessive germ cell death will lead to diminish spermatogenic function and decrease the quality and quantity of sperm. Excessive germ cell death triggered by various regulatory stimuli, including deprivation of gonadotropins and intratesticular testosterone or by estradiol treatment in rats via apoptosis.

生精细胞凋亡是哺乳动物精子发生过程中的一个显著特点，其生理性凋亡对于去除受损的生精细胞，控制细胞数量和保证精子的质量具有重要意义，但生精细胞的过度凋亡可引起生精功能低下，使精子的数量和质量降低。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。