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The cells were passaged no more than 5 generations,and the cell freezing density is about 3×106 cell/ml, sample numbers of each kind are between 30 and 60, conserved numbers are 150-180 ampules,biological characters of fibroblast were analysed according to the request of constructing cell line. The results were as follows: 1 Cultured cells'morpha is typical fibroblast,which is fusiformate or unregular triangle, cells is radiant,flaming or rotative during growing .Qingyuan flesh chicken's cytoplasmic tubercle is much more and shorter in contrast with Zang chicken and Langshan chicken,that can be regarded as different character from others.

细胞冻存代数在5 代以内，冻存密度为3×106个细胞/ml，每个品种的样本含量在30～60 个之间，保存管数达150～180 支，根据建系要求，对成纤维细胞的生物学性状进行分析，所得结果如下： 1 培养细胞形态为典型的成纤维状，为梭形或不规则三角形，细胞在生长时呈放射状、火焰状或旋涡状走形，清远麻鸡的细胞形态与藏鸡和狼山鸡相比，胞质向外伸出的突起较多，且较短。

If the current cell is the leftmost cell, SHIFT+TAB selects the last cell in the previous row.

如果当前单元格是最左边的单元格，Shift+Tab 就会选择前一行的最后一个单元格。

Methods: Study the newly found cell with the weak silver carbonate staining method of del Rio-Hortega. Using transmission electron microscopy, compare the newly found cell with the atypical cell found by Marc Lenoir and Philippe Vago, and extend young SD rat model to adult SD rat, young and adult Wistar rat,and extend drug of Neomycin to Amikacin to study the universality.

采用经典的小胶质细胞特殊染色方法——银染法(Hortega氏碳酸银法)对大鼠耳蜗药物损伤后出现的新细胞进行研究；通过透射电镜与Marc Lenoir和Philippe Vago实验动物模型中的"非典型细胞"进行了比较与扩展研究；运用逆转录PCR技术检测耳蜗基底膜上神经干细胞标志物nestin的表达情况，对新细胞的来源及其与神经干细胞或nestin的前体细胞的关系作进一步研究。

The level of VEGF mRNA was elevated after 6h when 8Gy irradiation was processed and maintained to 72h. Sense and anti-sense expression vector were transfected into vascular endothelial cells and stable cell lines were established. Cell line transfected sense expression vector of VEGF_(165) gene showed reduced inhibition of prolifetation, MNF, MNCF and apoptosis compared to that of control vector, while opposite results were obtained in anti-sense transfected cell line.

转染正义表达载体的ECV-304稳定细胞系经过4Gy γ射线照射后，与转染空载体的细胞相比，细胞增殖抑制率减弱，MNF、MNCF以及凋亡减少；而转染反义表达载体的细胞系与转染空载体的细胞相比，细胞增殖抑制率增强，MNF、MNCF以及凋亡增加。

In contrast to the monolayer cell culture system, the livingness of VSMCS cultured in "three-dimensional" cell culture system had no statistical difference, but collagen restrained cell proliferation.

三维培养VSMCS与经典的单层培养相比，细胞活力无统计学差异。但细胞增殖受到一定程度的抑制。

Methods In vitro, human gastric cancer cell line SGC-7901 in log phase was intervened by As_2O_3 (final concentrations in order:1.0,5.0,10.0μmol/L) and 5-Fu (final concentration:50mg/L) respectively alone or in combination, and incubated for 24h、 48h、 72h. Blank group is set up. The cell apoptosis and cell cycle changes were observed by flow cytometry, and the expression of apoptosis-related gene Bcl-2 was analyzed by immunocytochemical method and absolute gray scales quantitative method.

将对数生长期的人胃癌细胞株SGC-7901(终浓度为2×10~5)经低、中、高三种浓度的As_2O(终浓度分别为1.0，5.0，10.0μmol/L)和5-Fu(终浓度50mg/L)单独及联合处理，设空白对照组，分别孵育24h、48h、72h后，收取标本采用流式细胞术测定细胞凋亡率及细胞周期变化；免疫组化绝对灰度定量分析检测凋亡抑制基因Bcl-2的表达。

Choline and folic acid deficiencies could induce cell death in human lymphoblast cell line, and 6 μmol/L CC and 120 nmol/L FA was the optimal combination for keeping the lowest level of APO and NEC in human lymphoblast cell line.

胆碱与叶酸不足可诱导人成淋巴细胞株细胞死亡，6 μmol/L氯化胆碱及120 nmol/L叶酸是维持体外培养的人成淋巴细胞株APO及NEC频率在最低水平的最适组合浓度。

Methods The stablishment of lymphoblast cell lines was by EBV-immortalization and subtyping of the cell lines by cell fusion, mytomycin C inhibition analysis and/or immunoprecipitation and Westemn blot.

方法将我国3例FA患者外周血细胞用EB病毒活化法建立原始B-淋巴细胞系，采用丝裂霉素生长抑制试验和细胞融合后MMC抑制试验的互补试验进行分型；利用单克隆抗体免疫沉淀法和Western blot法进行分型。

They had higher SCE rate and micro nuclei rate, with 14.12times per cell, and 13.28 times per cell, respectively for SCE rate and 25.2% and 21.2%, respectively for micro nuclei rate, After treated wito mitomytin, they had even higher SCE (38.3、37.2)and micro-nuclei rate(10.7%,10.2%) than normal β－lymphoblast cell line.

现常用细胞EB病毒体外感染外周血淋巴细胞建立淋巴母细胞株，具永生性和不致瘤性，保持原有细胞的生物学特性，为实验室研究提供可信的材料[2]。

It publishes original research reports, reviews in the following areas: cell cycle regulation, cytokines, erythropoiesis, gene therapy, general hematopoiesis, granulopoiesis, hematological malignancies, immunobiology, immunotherapy, lymphopoiesis, megakaryocytopoiesis, microenvironment, monocyte development, molecular genetics, signal transduction, stem cell biology, and experimental as well as clinical stem cell transplantation.

该杂志刊载细胞周期调控、细胞因子、红细胞生成、基因疗法、全身血细胞生成、粒细胞生成、血液肿瘤、免疫生物、免疫疗法、淋巴细胞生成、巨核细胞生成、微环境、单细胞生成、分子遗传学、信号传导、干细胞生物学、实验与临床干细胞移植方面的原始论文及综述等。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。