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In the EPC repair group, 1010/L passage 3 rabbit bone marrow mononuclear cell suspension was injected to two anastomotic stomas of the new urethra, 0.1 mL for each stoma. The subcutaneous tissue was interruptedly sutured to the formed urethra using 0/6 DG suture. In addition, 0.5 mL bone marrow mononuclear cell suspension was added to the region between each anastomotic stroma and newly repaired urethra. The same procedure was performed in the model group except that bone marrow mononuclear cell suspension was replaced by cell-free medium.

细胞修复组将传至第3代的兔骨髓源单个核细胞浓度调整至1010 L-1,注射于新尿道两端的吻合口处,每处0.1 mL,0/6DG线间断缝合皮下组织覆盖成形后的尿道,并在近吻合口处和新修复尿道之中间处注射细胞悬液,每处0.5 mL;模型组在同样位点注射等量的空白细胞培养液。

It was known that pyridine and Triton-x100 had a bad effect on the cell growth and lycopene production were studied. The results indicated that pyridine and triton-x100 inhibited cell growth and lycopene synthesis;β-ionone increased cell growth and lycopene synthesis when the concentration was 0.05 ml/L, but when the concentration became higher, there would be a decrease on the lycopene output; span-20 increased cell growth and lycopene synthesis effectively when the concentration reached 2.0ml/L, and the lycopene yield became 2.4×10~(-2) mg/L which was 3-fold than control.

研究了培养基中加入不同浓度的小分子效应物吡啶、Triton-x100、Span-20、β-紫罗酮、异烟肼对番茄红素产量的提高作用,结果显示培养基中加入很低浓度的吡啶(0.1ml/L)就会对菌体产生很强的毒害作用;triton-x100对类胡萝卜素的合成也有很强的抑制作用,试验的浓度为0.2ml/L到5ml/L,结果发现它对菌体的生长和番茄红素的合成都有抑制作用;β-紫罗酮在浓度为0.05ml/L时有很小的促进作用,而浓度升高就有了比较强的抑制效果;span-20在液体培养基中的加量为2.0ml/L时,对番茄红素的合成有很强的促进效果,产量达到了2.4×10~(-2)mg/L,比原始菌株产量提高了2倍,而浓度进一步增大就产生了很强的抑制效果。

Fund Project: the National Natural Science and Technology Source Program, No. 2001DEA1006*Abstract: Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have no cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少,从细胞形态来看神经干细胞为圆形或椭圆形,无或有较短的突起,核质比大,核染色较深,形态上与其它种类的细胞没有明显的差异,并且未找到一种细胞表面特异性标志物,因此目前鉴定神经干细胞主要有以下3个方面:特异性神经抗原的表达,包括巢蛋白、Musashi、转录因子及细胞黏附分子;自我更新能力,包括单细胞克隆分析、BrdU标记S期细胞;多向分化潜能,包括免疫细胞化学法、聚合酶链反应色法。

Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少,从细胞形态来看神经干细胞为圆形或椭圆形,无或有较短的突起,核质比大,核染色较深,形态上与其它种类的细胞没有明显的差异,并且未找到一种细胞表面特异性标志物,因此目前鉴定神经干细胞主要有以下3个方面:特异性神经抗原的表达,包括巢蛋白、Musashi、转录因子及细胞黏附分子;自我更新能力,包括单细胞克隆分析、BrdU标记S期细胞;多向分化潜能,包括免疫细胞化学法、聚合酶链反应色法。

In this paper, the least squares approximation method is introduced to predict the trend line of carrier movement. Then the yarn paths are analyzed systematically. Finally, the three kinds of unit cell, the interior cell, the surface cell and the corner cell, are established.

采用最小二乘法分段对携纱器的相关运动位置点进行拟合,得到编织过程中纱线的空间运动规律,在此基础上,获得的预制件结构的单胞模型,包含内部单胞,表面单胞和棱角单胞。

Megaspore mother cell just enters leptotene as The meiosis of microspore mother cell develop into tetrads . The meiosis of megaspore mother cell develop into pachytene while that of microspores develop into a single nuclear adjacent to cell wall.

当白杨小孢子母细胞减数分裂至四分体时,大孢子母细胞开始进入减数分裂细线期;而当小孢子发育至单核靠边期上时,大孢子母细胞减数分裂达到粗线期。

The amplification failure rate of the single cell 5-plex nest PCR is significantly associated with the difference of the methods of cell lysed. Using appropriate method to lyse cell can improve the accuracy of single cell PCR.

结果表明单细胞PCR模板制备与PCR扩增失败有密切关系,选用合适的细胞裂解方法可提高单细胞PCR的准确性。

Both the new peniculus 1 and the old haplokinety separated at the telophase.The two germinal rows germinal rowsfor both the daughter cells appeared almost at the same time;(2) The macronucleus became shorter and thicker, finally oblate during the division of the cell. Then the macronucleus restored its original shape, band-like form. The micronucleus were divided into two before the macronucleus division;(3) The original scopula and stalk contributed to the old cell. The new cell formed scopula gradually after the cell division.

同时,新仔虫的第一咽膜(P′1)也开始由老单毛基索复制,并在细胞分裂后期与老结构分离;(2)大核在虫体分裂过程中由长带状逐渐缩短变粗至扁圆形,于虫体即将分开时迅速拉长,然后分裂为二个新大核;小核分裂先于大核,在两仔虫口纤毛器即将分开时完成;(3)原帚胚及柄归属老仔虫,新仔虫的帚胚在虫体分裂后逐渐形成,柄内肌丝则在柄鞘形成后逐渐长出。

The period length of lignin deposition was:fiber<stone cell<metaxylem vessel<ground tissue parenchyma cell.The iignin content of each kind of cell wall increased constantly accompanying with the thickness increasing of cell wall.

结合场发射和透射电镜的研究结果表明,无论初生壁还是次生壁发育阶段,CMTs排列方式的变化与CMfs沉积方向的变化具有明显的一致性。5。

The wood structures of Lauraceae were characterized by most diffuse porous; most pores solitary, less multiple and cluster pores; simple and scalariform perforation with few bars; most solitary pore; alternate intervessel pitting; most of rays multiseriate, less uniseriate; all of rays heterogeneous, mostly heterogeneous Ⅲ and Ⅱ type rays; most of axial parenchyma paratracheal type, less banded or marginal type; oil cell and mucilage cell in axial parenchyma cell or ray cell; mostly fiber tracheid and libriform fiber, less septate fiber.

结果表明:除檫木外,其它木材的管孔分布均为散孔材,具较高的单孔率;导管分子穿孔板兼有单穿孔和梯状穿孔2种或者仅具有单穿孔;导管间纹孔式为互列;导管-射线间纹孔式类型丰富,主要为刻痕状和大圆状。木射线有单列和多列射线2种类型,单列射线稀少、短,多列射线数量多;射线组织主要为异形Ⅲ和异形Ⅱ;轴向薄壁组织以傍管状为主,少数有带状或轮界状。油细胞和粘液细胞普遍存在于射线薄壁细胞或轴向薄壁细胞中。木纤维由韧性纤维和纤维管胞组成,部分树种具分隔纤维。

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I didn't watch TV last night, because it .

昨晚我没有看电视,因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来,在北京的很多小区里,电梯液晶电视被撤了下来。

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我比喻得过头了。