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It was seen that strain 6055 generated three kinds of dextransucrases whose molecular weights were 151 kDa,142kDa and 117kDa,respectively.Among them,the former two ones were found both in supematants and cell wall,while the later was only found in the fermentation liquor.Strain DM 1-2 synthesized two kinds of dextransucrases,and their molecular weights were 183kDa and 142kDa,respectively.The former existed both in cell wall and out of cell,but the later only was located on cell surface.Strain PC 13 produced three kinds of dextransucrases,and their molecular weights were 148kDa, 138kDa and 115kDa,respectively.The former two ones were ectoenzyme while the later was linked to cell.Resembled to strain 6055,strain L4 produced two types of dextransucrases coexisting in supernatants and cell wall with molecular weights of 145 kDa and 136kDa,respectively,but the dextransucrase of 115kDa was only found in free-cells supernatants.

结果显示，菌株6055可产生分子量分别为151kDa、142kDa和117kDa的三种葡聚糖蔗糖酶，其中前两者同时存在于上清液中和细胞表面，而后者只存在于上清液当中；菌株DM1-2可以产生两种葡聚糖蔗糖酶，分子量分别为183kDa和142kDa，前者同时存在于上清液中和细胞表面，而后者仅能存在于细胞表面；菌株PC13可以产生分子量分别为148 kDa、138 kDa和115kDa的三种葡聚糖蔗糖酶，前两者属于胞外酶，而后者与细胞相连；与菌株6055相似，菌株L4可以产生两种同时存在于上清液和细胞表面的葡聚糖蔗糖酶，分子量分别为145 kDa和136kDa，而分子量为115kDa的葡聚糖蔗糖酶只存在于上清液中。

Compared with classical cytotoxic T lymphocytes , NK cells are superior in the following aspects: NK cells are among the first cell types to be activated after intracellular pathogen stimuli, the activity of NK cells peaked between 1 to 3 days, retained during the first 7-10 days, and the T cells response emerged only after 7 days when the NK cell responses begin to decline; though comprising of only 10％-15％ of peripheral lymphocytes, NK cells mobilize most of the cell populations in a strong and high level response to different invasions, and act as key factors at early defense before a specific immune response could mount, while only certain clones were involved in T cell response; NK cells kill virus-infected or malignant transformed cells without pre-sensitization and without restriction by major histocompatibility antigen, which is usually a mechanism of immune escape to T cell recognition by virus-infected or tumor cells, thus NK cell in complementary with T cells play crucial roles in tumor and virus eradication.

NK细胞以其强大的细胞毒活性为特征，与细胞毒性T细胞相比，NK细胞具有如下特点：NK细胞虽然只占外周血淋巴细胞的10-15％，但是对刺激性因素产生应答的过程十分迅速，一次可以调动大多数细胞共同参与，而不是几个克隆的活化，免疫应答的强度高；病毒感染或恶性转化细胞的一个主要特征是细胞表达的MHCⅠ类分子下降或消失，并借助这一机制逃避特异性T细胞应答的识别，NK细胞介导的杀伤活性无需抗原刺激，不受MHCⅠ分子表达的限制，从而与T细胞应答互为补充发挥免疫防御的功能；NK细胞的免疫活性可以被多种细胞因子上调，其中IFNα，IL-2，IL-12，IL-15和IL-18具备更强的作用；NK细胞具有强大的细胞因子分泌功能，对于启动T细胞特异性应答必不可少。

Its main features are: algae body as a single cell or even into a variety of groups; cell walls composed of silica and pectin, but also by the structure of the shell and the shell under the combined set of the Cheng, vertical section view was "soap-box"-shaped; in the upper and lower shell surface of the shell are patterns arranged in patterns on both sides of the main mode of symmetry and radial symmetry is divided into two categories, for the classification of the most important basis; be able to campaign Type in the shell has a shell side seam, can not be changed no shell seam types; pigment body sheet or ribbon, 1 or 2, or for multiple small discoid, yellow green or brown, containing chlorophyll a, c, also contains fucoxanthin and silicon A flavin and other photosynthetic pigments; storage material mainly for oil droplets; reproduction mainly for cell division and cell division in diatoms obvious features are: each cell division, resulting from two sub-cells,, only one and the mother cell and so big, the other one is smaller.

其主要特征是：藻体为单细胞或连成各式群体；细胞壁由硅质和果胶质组成，而且在结构上都是由上壳和下壳套合而成，纵断面观呈"肥皂盒"形；在上下壳的壳面上有花纹，花纹排列的方式主要分为两侧对称和辐射对称两大类，为分类上的最重要依据；能运动的种类在壳面都有壳缝，不能动的种类均无壳缝；色素体片状或带状，1个或2个，或为多个小盘状，黄绿色或黄褐色，含叶绿素a、c，还含有墨角藻黄素和硅甲黄素等光合色素；贮藏物质主要为油滴；繁殖方式主要为细胞分裂，硅藻的细胞分裂的明显特点是：每次细胞分裂所产生的2个子细胞中，仅有1个和母细胞等大，另1个则稍小。

KIR bind corresponding HLA-Ⅰmay hame four results followed:1、When inhibitory KIR bind HLA-Ⅰand there has no activatory KIR bind it,cell isn\'t dissolve because inhibitory signal access was done;2、When activatory KIR bind corresponding HLA-Ⅰand there has no inhibitory KIR bind it,form activate signal,lead cell dissolve.3、If activatory or inhibitory receptor both bind HLA-Ⅰ,both signal access is make done,when activatory signal play the main role,NK cell still actived,lead target cell dissolve.4、If activatory or inhibitory receptor both bind HLA-Ⅰ,both signal access is make done,when inhibitory signal play the main role,or both signal access don\'t play,NK cell do not make effect.this is NK cell recognice nomal tissue.

与活化型KIR受体相比，抑制型KIR受体与HLA-Ⅰ类抗原结合的亲和性更强，当抑制型和活化型KIR受体识别和结合同一HLA-Ⅰ类抗原分子时，以抑制作用为主。KIR与相应HLA-Ⅰ结合后可能产生以下4种情况：①当抑制型KIR与HLA-Ⅰ结合而无活化型KIR与HLA-Ⅰ相互作用时，因抑制信号通路启动而不产生细胞溶解；②当活化型KIR与靶细胞表面的相应HLA-Ⅰ结合同时无抑制型KIR与HLA-Ⅰ的相互作用时，形成刺激信号通路，导致靶细胞溶解。

Results showed that L-THP possessed negative inotropic and negative chronotropic action, lowed left ventricular pressure and it's infinitesimal calculus, decreased heart output in per min., shorten 〓 and 〓 of action potential of ventricular papillary muscle of guinea pig and even the plate-phase of action potential disappeared in larger dose group , but prolonged 〓. we studied transmembrane transportation of 〓 after administering L-THP , L-THP not only depressed uptake and increased release of Ca 2+ in normal myocardium, but also it showed stronger inhibition in cell injured by ISO, and fully eliminated the effect of ISO increasing uptake and decreasing release of 〓. By detecting the 〓 content of ventricular single myocyte using fluorescent probe Fluo-3/AM, results correspond to that done above, L-THP decreased 〓 content inside of cell significantly. Using whole-cell mode, we observed the effect of L-THP on Ica in ventricular single cell, it was found that L-THP decreased Ica, the effect increased with increasing it's dose, and had voltage, frequency-dependency. Dose highly corelated to effect, corelative constant is 0.87. When washing cell with normal tyre's solution, Ica could recover, which proved the blocked action of L-THP on calcium channel once again. Meanwhile, L-THP reduced production of MDA in heart injured by ISO in dose-dependent manner, improved content of GSH-Px, in case tissue or cell free from free radical damage.

结果显示，该药物具有负性肌力和负性频率作用，降低左室压及其微分，终使每分心输出量减少；缩短豚鼠心室乳头肌动作电位时程的〓和APD〓，大剂量使动作电位平台消失，〓延长；同位素示踪法研究证实L-THP抑制〓摄取，促进〓释放，降低细胞内的〓含量；单通道水平的研究证实，L-THP减少豚鼠心室肌单细胞内向钙电流，且具有剂量，电压，频率依赖性，其作用易于洗脱，药物剂量与Ica也具有高度相关性（相关系数r为-0.87）；L-THP还可抑制ISO损伤心肌组织所致的丙二醛含量增加，增加谷胱甘肽过氧化物酶的量，使组织细胞免受损伤。L-THP阻断L-型〓通道，抑制〓的摄取，促进〓的释放，降低细胞内〓含量L-THP作用的离子基础。

In this paper, we present an access cell selection approach, which is based on cell sojourn time estimation. The approach selects access cell according to the cell sojourn time estimated. Compared with residual dwell time approach and power level offset approach, our access cell selection approach has a much smaller probabfiity of erroneous access cell selection, thus a better performance.

在本文中，我们提出了一种基于移动台小区驻留时间估计的接入层次选择方法，这种方法依据移动台在小区中的驻留时间估计值来进行接入层次选择，与剩余驻留时间方法与功率小区偏移方法相比，其小区选择错误概率明显要低，具有较好的性能。

Because of this situation, we suggested that the construction of human embryonic stem cell research regulations in Taiwan should be of first priority and illegal use of human embryo or unpatentable types of human embryonic stem cell inventions should be regulated by law. Moreover, following the trend of stem cell technology, the regulation of cell therapy, gene therapy and analysis or diagnostic technologies related to stem cells should be amended to support stem cell research and research foundation, research awards or preference of business investment should be established to improve development of stem cell industry in Taiwan.

据此，本研究建议我国应优先建制人类胚胎干细胞研究管制规范，并以法律明订违法使用人类胚胎之情况或不予专利之人类胚胎干细胞发明态样，另一方面配合干细胞技术的应用趋势，对於干细胞相关的细胞疗法、基因疗法以及干细胞特性检测等潜在发展领域进行相关规范之增修，并且针对干细胞产业设立研究基金、拟定奖励研究或优惠投资方案，以达到促进干细胞产业发展之政策目的。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

Pathology findings:there were 294 cases of clear renal cell carcinoma (83.76%),including 2 cases of cystis renal cell carcinoma ,27 cases of Grannular renal cell carcinoma ( 7.69 %), 14 cases of papilliform renal cell carcinoma , 6 cases of the mixed type renal cell carcinoma,10 cases of other cell types .

病理结果：透明细胞癌294 例(83.76%，其中囊性肾细胞癌2例)，颗粒细胞癌27 例(7.69%)，乳头状肾细胞癌14例(3.98%)，肾混合细胞癌6例(1.7%)，其它癌10例。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。