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The slow progress of researches on pulmonary stem cells up to date has its root in the complex structure of pulmonary tissues and respiratory tracts, which is demonstrated by the facts that the respiratory system is composed of at least 40 types of cells, plus that the growth, renewal, and regeneration rate or capacity of respiratory epithelium are extremely limited. Mean while, there have been no specific stem cell markers for pulmonary and lung cancer, so researchers rely on stem cell markers borrowed from other systems to search for stem cells in pulmonary system.

目前对于肺干细胞的研究进展缓慢，究其原因主要是肺组织和气道具有复杂的内在结构，呼吸系统至少包含40种不同类型的细胞，呼吸上皮本身只有极低的生长更新率和非常有限的再生能力，同时肺干细胞的表面标志物也缺乏特异性，只能借助其他组织已知的干细胞标记物进行对比观察。

Western-blot and immunohistochemical assay methods were employed to detect the expression of FHIT. Apoptosis and cell cycle of cell were analyzed by FCMS. Morphological changes were observed by compound and electron microscope.

然后用流式细胞仪，普通光镜及透射电镜对获得稳定FHIT蛋白表达的转染组细胞和空真核表达质粒转染组细胞及未转染的亲本细胞凋亡的状况进行了考察，另外还通过流式细胞仪检测了三组浙江大学硕士学位论文细胞生长周期的惰况。

Signal 1 contain compound substance by the human organization of APC cell surface Ⅱ kind molecular present antigen, identify for T cell.

信号1由APC细胞表面的人类组织相容复合物Ⅱ类分子呈递抗原，供T细胞识别。

The recombinant eukaryotic expression vector pcDNA3.1-Myc-His B/tPA can stable express tPAcDNA in transfected hUVEC cell strains and can secret compt tPA protein. Experiment III The Effects of Transfected tPA Gene on Vascular Smooth Muscle Cells Proliferation and Migration in VitroObjective To investigate the effects of transfected tPA gene on vascular smooth muscle cell proliferation and migration in vitro and to provide a basis for further study on preventing vein graft restenosis.

结论构建的pcDNA3.1-Myc-HisB/tPA基因新型真核表达载体，能在HUVEC中表达，其表达具有高拷贝、稳定性好的特点，获得的表达蛋白活性较高，提示本载体和稳定表达tPA基因的人脐静脉内皮株能够作为基因治疗工具用于哺乳动物的实验研究。

Methods: The mesencephalon of rat with conceptus age of 13 days was taken out and dissociated into single cell suspension mechanically, and then cultured in serum-free medium. Fetal bovine serum was used to induce cell differentiation. The isolated cells and their progeny were observed by using immunofluorescence technique.

取孕14 d胎鼠大脑皮层，吸管吹打机械分离制成单细胞悬液，采用无血清培养和胎牛血清诱导分化，应用免疫荧光技术鉴定神经干细胞及其分化的子代细胞。

Concordantly, reprogramming of granulosa and theca cell lineages into Sertoli-like and Leydig-like cell lineages occurs with testosterone levels comparable to those of normal XY male littermates.

因此，似乎组织形态学和肠道对氧化损伤的响应是由对氧化裂解的识别来维持的，并且通过JAK—STAT和JNK通道的信号导致了肠内干细胞的增殖和修复。

A549 cells are more sensitive to CDDP-induced apoptosis(P.01) and less sensitive to THP andβ-Ele-induced apoptosis than A375p(P.01, P.05 respectively). Condensation and crack of nucleus and apoptotic bodies appeared in apoptotic cells of A549 and A375p cell lines in all treated groups and necrocytosis were to be seen in some groups. Fluorescence imaging experiments demonstrated that accumulation of iASPP in cytoplasm but little distribution in nucleus in all groups. untreated groups in two cell lines presented a bright-green colour,followed by CDDP-treated groups,and THP-treated groups is the darkest one in all groups.

免疫荧光显示，未加药物处理的A549和A375p细胞胞质染色均匀呈亮绿色，核区较暗；CDDP及β-Ele处理组细胞为暗绿色，其中可见皱缩的凋亡细胞；THP处理的细胞胞质荧光弱呈灰绿色，由于THP嵌入DNA自发红色荧光与二抗的绿色荧光中和，胞核被染成桔红色，结果提示药物处理后A549和A375p细胞中iASPP的表达有不同程度的下降。

The drug study was condign to carry out on 8~-10~of HepG2.2.15 cell culture,inwhich phase the cell was in summit of growth.

培养第8～12天的HepG2.2.15细胞处于生长高峰期，在此期进行药物实验较适宜。

To elucidate the role of full-length functional AZ in T cell activation, we examined whether conditional expression of full-length AZ protein could elucidate the hallmarks of Jurkat T cell activation.

为了探讨全长具功能性的抑酶在T细胞活化过程中所扮演的角色，本论文研究抑酶的过表现对於Jurkat T细胞活化指标特性的影响。

Results Conglobate fusiform squamous cell and meconium corpuscles on arteriole and micrangium of lung could be found in all 2 cases ofammiotic fluid embolism.Squamous cell on uterine blood vessel could be found in one case.

结果 2例均见肺小动脉及毛细血管内有成团梭形鳞状细胞，并见胎粪小体，1例在子宫血管内见鳞状细胞。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。