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Besides, the series of synthetic compounds were tested for their capability to suppress growth of human hepatocellular carcinoma cell line Hep 3B and Hep G2, and myeloid leukemia cell line HL-60. The results showed compound 20, N-(2-amino- 3-nitrophenyl)cantharidinimide, exhibited more potent than others, but it was still less effective than cantharidin.

此外也利用斑蝥胺的衍生物对人类肝癌细胞Hep 3B和Hep G2，以及血癌细胞HL-60的细胞毒性进行测试，结果显示化合物20 [N-(2-amino-3-nitrophenyl)cantharidinimide]有较好的抑制作用，但是效果仍未比斑蝥素好。

In one kind of viral life cycle, the virus first binds to the cell's surface, then penetrates the cell and sheds its capsid.

在某种病毒的生命史中，病毒先是附着于细胞的表面，然后穿透细胞并蜕下其病毒外壳。

The losing of balance of cell proliferation and cell death is closely related to the carcinogenesis.

肿瘤的发生与细胞增殖和死亡之间的比例失衡密切相关。

Methods The peripheral blood lymphocytes,sera of 55 patients with rheumatic carditis and a specific antigen from group A streptococcal cell membrane were prepared and used as stimulants to test the heart tissue cell procoagulant activity in culture.

用风湿性心脏炎患者的外周血淋巴细胞、血清及A组乙型溶血链球菌膜抗原作为刺激物，测定体外培养的人胚心肌细胞促凝血活性。

HPCA was measured by using a one-stage plasma recalcification clotting time.Results The peripheral blood lymphocytes from rheumatic carditis patients can stimulate the procoagulant activity of human heart tissue cell in vitro.But the sera of rheumatic carditis patients and group A streptococcal cell membrane can not increase HPCA.

结果 患者的外周血淋巴细胞能使HPCA明显升高；静止期风心病、风湿性关节炎、其他疾病对照组及健康对照组患者的外周血淋巴细胞均无此特性，迁延活动型风湿性心脏炎患者的血清及A组乙型溶血链球菌膜抗原对HPCA也无直接影响。

The effect of carotenoid on the bcl-2 gene expression of breast cancer cell lines MCF-7 cell was determined by the method of RT-PCR.

采用RT-PCR方法检测类胡萝卜素对MCF-7细胞株的Bcl-2基因表达的影响，均呈不同程度的下调。

Methods The target DNA sequence of yrdC was obtained from human spleen tissue by using RT-PCR,construct high titer recombinant adenovirus Ad-yrdC by using AdEasy adenovirus carrier system,detect yrdC protein expression by using Western blot and immunohistochemical method,set up control group and transfected Ad-Null group,and study the effects Ad-yrdC on BGC-823 cell line by drawing cell growth curve and MTT chromatometry.

方法用RT-PCR方法获取人脾脏组织yrdC基因序列，采用AdEasy腺病毒载体系统构建携带yrdC基因的高滴度重组腺病毒Ad-yrdC，将其转染胃癌BGC-823细胞，应用Western blot和免疫组织化学法检测yrdC蛋白表达，设对照组和转染Ad-Null组，并分别绘制细胞生长曲线和MTT比色法研究Ad-yrdC对BGC-823细胞的影响。

Using this method, we observed the metabolic condition of cartilage cell and knew the effect of the serum with the various types and different concentration to the growth of cartilage cell.

利用体外细胞培养的方法进行生物学、医学领域的科学研究工作近年来在世界上甚为广泛，以软骨细胞培养的方法对软骨和骨关节疾病进行的研究也日趋深人，并已发展到分子水平。

In order to test this hypothesis, we isolated and developed stable self-renewable cell lines of mesenchymal stem cells from human fetal articular cartilage and identified the cell phenotype by FACS and we found that their phenotypic markers were identical to those of mesenchymal stem cells from bone marrow.

我们从胚胎的关节软骨中获得成纤维样细胞，在体外进行培养和扩增，并用流式分析的方法对其表面标志进行鉴定，发现其表面标志与文献报道的骨髓来源的间充质干细胞的表面标志相同。

Although the limited capacity of cartilage cell proliferation, its direct impact on the quality and quantity of in vitro amplification effect, cartilage cell growth environment shown by the different biological characteristics there are differences.

虽 然软骨细胞增殖能力有限，其质与量直接影响体外培养扩增效果，软骨细胞生长环境不同所表现出的生物学特性也有差别。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。