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Then, on the basis of modeling the manufacturing cell with the O-O method, the information model of the manufacturing cell is described with XML language. It provides a method of information exchange to the optimization and configuration of manufacturing cell together with the development of application system.

其次,在制造单元信息模型的基础上,使用XML语言对制造单元信息模型进行了描述,为制造单元的优选配置以及应用系统的开发提供了信息交换的方法。

We compared the changes of T reg cell levels pretransplant and short courses posttransplant, the changes of T reg cell levels between the acute rejectors and the stables,and the changes of T reg cell levels between CsA group and FK506 group.

对比术前和术后近期T reg细胞水平的变化,移植肾功能稳定组和急性排斥组T reg细胞水平的变化,CsA组和FK506组T reg细胞水平的变化。

Methods Phase-contrast microscopy, cell proliferation assay and wound -closure were used to study the process of cell proliferation and wound healing in a model of human retinal pigment epithelial cell damage.

应用人RPE细胞损伤模型、细胞增殖曲线法,在倒置相差显微镜下观察人RPE细胞在各种条件作用下的损伤愈合与增殖过程。

CTAB was used to pyrolyse plant cell and reacted with the released cell nucleic acid to form nucleic acid-CTAB complex, the cell extract was then deproteined with chloroform-isoamylol, at last RNA was selectively precipitated by LiCl.

采用CTAB裂解植物材料,与细胞中的核酸形成核酸-CTAB复合体,然后用氯仿一异戊醇去除细胞裂解液中的蛋白,最后采用LiCl选择性沉淀其中的RNA。

CTAB was used to pyrolyse plant cell and reacted with the released cell nucleic acid to form nucleic acid-CTAB complex,the cell extract was then deproteined with chloroform-isoamylol,at last RNA was selectively precipitated by LiCl.

但是一般高等植物组织内都含有多糖类物质,这些物质的存在严重影响RNA的提取,如果去除不净,将影响反转录、扩增等下游操作。

To understand the infectivity by porcine endogenous retrovirus with porcine skin fibroblast cell in vitro and in vivo, porcine skin fibroblast cell established by our laboratory were co-cultured with neo/HEK293 cell for the infection of RERV in vitro, and were subcutaneously transplantated to SCID (severe combined immuno-deficiency) mice for the infection of PERV in vivo, laying the foundation for valuation of biologic safety of xenotrans-plantation. The event of neo/HEK293 cells infected by PERV occurred during co-culture of porcine skin fibroblast cells with neo/HEK293 cells, expanding the rang of the infection of porcine endogenous retrovirus. Afterpig cells transplantated subcutaneously in SCID mice, the microchimerism (78.57%) of pig cells occurred widel, and there was phenomena of integration of PERV provirus (85.71%) in several organs or tissues remote from the injected sites, indicating infection of PERV in SCID mice in vivo. yet, there is no evidence of active viral replication in analysis of PERV env RNA of these tissues or organs.

为了解猪皮肤成纤维细胞PERV在体外和体内的感染性,通过建立猪皮肤成纤维细胞系,将所建细胞系与人胚胎肾293细胞体外共培养,并移植于严重联合免疫缺陷鼠皮下进行猪皮肤成纤维细胞PERV的体外和体内感染性实验,结果表明,猪皮肤成纤维细胞与人胚胎肾细胞共培养过程中,猪内源性逆转录病霉感染人胚胎肾细胞,进一步证实和拓宽了猪细胞PERV感染人细胞的范畴;猪皮肤成纤维细胞移植SCID鼠皮下后,导致SCID鼠发生猪细胞微嵌合(78.57%)和PERV在体内感染(85.71%)并且波及远离移植部位的多种组织或器官,但是并未检测出SCID鼠组织中表达PERV env RNA。

A number of lines evidence indicate that calcium treatments maintain the structure and function of the cell walls, increase the fruit firmness, decrease the activity of the cell wall degration enzymes and ethylene production, maintain the integrity and stability of the membrane, activate some oxidases, induce phytolexin and regulate cell function as a signal mobecular.

钙的防腐机制在于对果蔬采后腐烂的控制,保持细胞壁的结构与功能,增强果实硬度,降低细胞壁降解酶的活性,抑制呼吸,降低乙烯生成量,保持细胞膜的完整性,激活体内的某些氧化酶,诱导合成植保素,作为信号分子,调节细胞功能。

It has beenconfirmed that the occurrence of basal cell carcinoma, small cell lung cancer,pancreatic cancer, prickle cell carcinoma, prostatic carcinoma, carcinoma of bladder,medulloblastoma and gastric cancer of mankind have a close relationship with the abnormal activation of Sonic hedgehog signaling pathway.

现已证实人类皮肤基底细胞癌、小细胞肺癌、胰腺癌、口腔鳞状细胞癌、前列腺癌、膀胱癌、髓母细胞瘤、胃癌等肿瘤的发生与sonic hedgehog信号通路异常激活密切相关。

According to the structure of wood cell wall, the preparation of aerogel-type wood mainly includes three steps, namely the destruction of wood cell wall in S3 layer, the expansion of wood cell wall and supercritical drying.

根据木材细胞壁的结构,气凝胶型木材的制备主要包括3个步骤:木材细胞壁S3层的破坏;木材细胞壁的膨化;超临界干燥。

The cell outline was collected with two-step expansion model of modified Snake,at the same time;greedy algorithm was applied to reduce the operative time of procedure.Upon the basis that the segmentation of wood cell intracavity and edge was finished,wood cell wall was identified.

利用改进的Snake两步膨胀模型对细胞轮廓进行提取,同时应用贪婪优化算法,减少程序运行时间,在完成对木材细胞内腔和边缘分割的基础上,完成对木材细胞壁的识别。

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