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Cell-based therapy is a promiseful option.This review will focus on the present status of cell-based therapy.Myoblast transfer therapy is hindered by minimal distribution of cells after injection,immune rejection,and poor cell survival.The drawback of bone marrow...

成肌细胞移植面临的主要限制是注射后细胞分布差，免疫排斥和细胞存活率低；骨髓干细胞移植需要解决横向分化的低效率问题；而肌源性干细胞看来能更有效地再生表达dystrophin的肌纤维。

Results bFGF 2.5ng/mL promoted ECV-304 cell migration. Cell pseudopodia was increased and cell skeleton was rearranged.

结果 bFGF 2.5ng/mL促进ECV-304细胞迁移，迁移细胞突起增多，细胞骨架发生重排。

As results, TEM showed characteristic ultrastructure morphology of apoptosis cell with chromatin marginal condense, nucleus pycnosis, mitochondrion vacuoles and cell shrinkage in IR injury group and no apoptosis cells in control group. Increased TUNEL-positive cell and typical DNA laddering were found and cells apoptotic rate were approximately 20% by flow cytometry assay in experimental groups.

值得探讨。本试验通过流式细胞仪、生物电镜、TUNEL法、RT-PCR以及DNA电泳等试验方法研究了带血供同种异体骨移植过程的细胞凋亡现象，并检测了Fas、FasL、Caspase-1、Caspase-3、Bcl-2等凋亡相关基因在IR损伤时的表达及变化情况，初步探讨了骨移植时IR损伤中细胞凋亡的发生机制及其在IR损伤中的生物学意义，希望为今后更深入的研究打下一定基础。

Under the phase-contract microscope, cell multi-l ayer was shown to be a refractile sheet, which means the capacities of biomateri al to attract cell and maintain the cell attachment.

相差显微镜下复层细胞表现为折光带，这反映生物材料引导细胞贴附并保持细胞贴附的能力。

RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.

结果：用诱导分化液作用后，未见小的、伴有突起的放射状形态的神经细胞；抗β Tubulin Ⅲ对经神经元胶质细胞诱导分化液作用后的人成肌细胞、增殖培养液培养后的各代人成肌细胞及仅含20 mL/L HS分化液分化形成的肌管细胞染色均为阳性； C2C12细胞β Tubulin Ⅲ抗体染色阴性；上述所有细胞抗Neurofilament Mr 68×103和抗GFAP染色均为阴性。

The results were that sucrose and glucose were two good carbon sources which could support the growth of Roselle cell, but soluble starch could not be assimilated by Roselle cell, and other nutrients assimilation by Roselle cell were far slower than that of two above carbon sources.

结果表明，蔗糖和葡萄糖在这些方面的表现基本相同，而可溶性淀粉由于植物细胞对其没有有效的水解利用手段，因而不能支持植物细胞的生长，从基质消耗水平上来看，也明显慢于上述两类碳源。

When the treated group were injected Safflor after ICH 6,12,24 hours,The number of caspase-3 positive cell around hematoma was gradually increased and the number of bcl-2/bax positive cell was gradually degraded and the number of TUNEL positive cell of Safflor group treated after ICH 6 hours were less than that of Safflor group treated after ICH 24 hours.

在不同时间给予红花注射液治疗脑出血大鼠，均能不同程度地促进脑出血大鼠神经功能的恢复，其中术后6h给药组治疗效果最好，其可能通过促进Bcl-2蛋白的表达，抑制Bax和Caspase-3蛋白的表达，从而抑制血肿周围的细胞凋亡。

The expression of survivin mRNA was assessed by RT-PCR; Cell cycle analysis; the cell cycle distribution of semiconfluent cell cultures was determined by follow cytomytric analysis of the content of propidium iodide-stained DNA.

本研究的目的就是通过bFGF刺激培养的人肝癌细胞株Bel-7402，并通过PI3K抑制剂wo咖annin阻断PKB的信号转导通路，以观察凋亡抑制蛋白Survivin mRNA的变化。

About a year after pollination, the generative cell of the four-celled male gametophyte undergoes division, giving rise to two kinds of cells—a sterile cell and a spermatogenous cell.

在授粉后大约一年，四个中生殖细胞-细胞男性的配偶体接受区分，引起细胞的二个类型-一个不毛的细胞和一个 spermatogenous 细胞。

The apoptosis of 80 mg/kg ﹒ d group was mainly located in spermatogenous cell and first spermatocyte; the apoptosis of 120 mg/kg ﹒ d group was mainly located in spermatogenous cell , first spermatocyte and spermatocyte of the second order; the apoptosis of 240 mg/kg ﹒ d group was mainly located in spermatogenous cell and first spermatocytes, and seemingly, the apoptosis located in first spermatocyte was dominant; whereas the apoptosis of 480 mg/kg ﹒ d group was located in all levels spermatogenic cells.

结果表明：各试验组小鼠生精细胞凋亡指数随着浓度的升高有增加的趋势，而且与对照组差异显著（ P ＜0.05或 P ＜0.01）。80 mg/kg · d 组凋亡细胞主要定位于精原细胞和初级精母细胞；120 mg/kg · d 组凋亡细胞主要定位于精原细胞、初级精母细胞和次级精母细胞；240 mg/kg · d 组凋亡细胞主要定位于精原细胞、初级精母细胞，以初级精母细胞为最多；而480 mg/kg · d 组凋亡细胞定位于各级生精细胞。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。