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Surface waxes of the pericarp were observed by scanning electron microscopy; the structure and composition of cell wall were studied using light microscopy, transmission electron microscopy and Fourier transform infrared microspectroscopy respectively; the changes of calcium in the cell wall were determined by atomic absorption and the integrity of cell membrane by conductivity meter respectively.

本论文采用扫描电子显微镜研究果实表皮蜡层的变化,用光学显微镜、透射电子显微镜和傅里叶变换红外光谱仪研究果实细胞壁结构和成分的变化,用原子吸收分光光度计测定细胞壁钙离子的变化,用电导率仪检测果实细胞的完整性。同时测定了果实酚类物质含量、多酚氧化酶(EC 1.10.3.1)活性、过氧化物酶(EC 1.11.1.7)活性,并分析了果实硬度、糖和酸含量等品质指标。

Results: Among 46 lesions there were 30 squamous cell carcinomas (65.2%), 8 adenocarcinomas(17.4%), 4 large cell carcinomas(8.7%), 3 small cell carcinomas(6.5%), and 1 mixe...

结果46例中,鳞癌30例(65.2%)、腺癌8例(17.4%)、大细胞癌4例(8.7%)、小细胞癌3例(6.5%)和混合性癌1例(2.2%)。

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2-4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L-1 after their number counted.

选取2~5代的小鼠胚胎成纤维细胞,待其至对数生长期倒掉或吸掉培养基,加入含10 mg/L丝裂霉素C的细胞全培养液5 mL,置37 ℃,体积分数0.05的CO2饱和湿度温箱中培养二三小时,在6孔板中加入0.1%明胶包被,放置30 min以上,倒掉或吸掉含丝裂霉素C的培养基,磷酸盐缓冲液清洗5遍,尽量除去丝裂霉素C,加入2 mL 0.05%的胰蛋白酶消化细胞,镜下观察,当细胞间出现裂隙,细胞变圆时(约2~4 min),立即加入等量的全培养液终止消化,并用吸管反复吹打,使之成为单细胞悬液,在细胞计数板中央放置专用的盖玻片,用玻璃虹吸管吸取细胞,让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液,至盖玻片下被液体充满为止。

Objective To determine the origin and function of multinucleated giant cell s and mononulear stroma cell s in giant cell tumor of bone.

目的探讨骨巨细胞瘤中多核巨细胞和单核基质细胞的起源及在肿瘤中的作用。

Now, I mainly engaged in myxobacterial genomic and bioinformatics analysis work. Myxobacteria is a single-cell microorganisms, but they act as multi-cell behavior (such as feeding, social movement, cells congregating and fruiting body formation, and so on) and own a wealth of secondary metabolites Product, those remarkable characteristics make myxobacteria important in the research fields of prokaryotes cell differentiation and development, biological evolution, as well as the drug development and research.

现主要从事粘球菌基因组与生物信息学分析方面的研究工作:粘细菌是单细胞微生物,然而却具有多细胞行为(如摄食、社会性运动、细胞聚集和子实体形成等)和丰富的次级代谢产物,这些行为特征使粘细菌在原核生物的细胞分化发育、生物进化以及药物开发研究中占有重要地位。

The outer neuroblastic layer before it divided to inner and outer nuclear layer and outer complexiform layer and gonglion cell layer showed a temporal PCNA positive which was related to proliferation. The three cell layers had another PCNA positive when finished time changed from outer to inner after their morphologic development basically completed( outer nuclear layer:11~15 days,inner nuclear layer: 8~16 days, gonglion cell layer:16~21 days).

PCNA显示在外成神经细胞层分化为内、外核层和外丛状层之前,和节细胞各有一次和增殖相关的阳性表达。3个细胞层在形态学的分化基本完成后,又出现了一次结束时间是由外向内变化的阳性过程(外核层:第11天至15天:内核层:第8天至16天,神经节细胞层: 16天到21天,这一次阳性表达过程可见到细胞形态向成年形态的转化。

Bone marrow stromal cells are cells got rid of hemopoietic stem cells in marrow cell of mature individual, which is approved to be multi-differentiation potential including transforming into neural stem cell, and differentiation into neurs and neuroglial cell.

骨髓基质细胞(Bone marrow stromal cells,BMSCs)是成体骨髓细胞中除造血干细胞之外的另一类骨髓干细胞,近年来的研究证明其具有多向分化的潜能,包括向神经干细胞转化,并可进一步分化为神经元和神经胶质细胞。

The first, many tuberculosises in glandular stomach were found besides liver cell degeneration, pneumoncongestion, epithelium cell degeneration of tubuli tenales, necrosis and kidney hemorrhage, cardiac muscle celldegeneration, heart hemorrhage, cerebrum hemorrhage, satellitosis and neuronophagia, cerebellum purkinje cell necrosis.

发现病例1,肝细胞变性,肺淤血,肾小管上皮细胞变性、坏死,间质出血,心肌纤维变性,间质出血,大脑出血,&卫星化&与&噬神经&现象,小脑浦肯野氏细胞坏死,还可见腺胃有大量的结核结节。

The human bile duct carcinoma cell line QBC_(939) was neurotropic. The co-culture model of DRG/nerve cell and carcinoma cells could simulate the process of the tumor cell going through the matrix and invading the nerve fiber.

结论DRG/神经细胞-肿瘤细胞共培养模型较好的模拟了肿瘤细胞穿过细胞基质,浸润神经纤维的过程,人胆管癌细胞QBC939细胞对神经纤维具有亲嗜性。

The results showed that ACP positive reactions were mainly deposited in lysosome, nucellar cell, cellular endomembrane, mitochondriurn, and endoplasmic reticulum in four tissues of the healthy shrimp. Furthermore, ACP activity was located in microvilli, and around partly lipid droplet in liver. AKP positive reactions were mainly deposited in cell membrane, nucellar cell, lysosome. endoplasmic reticulum and cellular endomembrane in four tissues.

结果显示,在健康的对虾体内,ACP依次出现在各组织中的溶酶体、细胞核、细胞内膜、线粒体、内质网以及肝脏组织中的部分脂滴周围及微绒毛中AKP依次出现在各组织中的细胞膜、细胞核、溶酶体、内质网以及肝脏组织中的脂滴周围及微绒毛中。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。