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When the strain grew in the medium containing glucose(40 g/L) as carbon source with tomato juice(3.6 mL/L), gingili oil(1.6 mL/L), Na2CO3(6 g/L)and phosphate buffer, at 28 ℃ with illumination for 120 h, its cell biomass, and carotenoid yield and content could reach 65.57 mg wet cell per mL medium, 9.896 μg per mL medium and 150.9 μg per gram wet cell, respectively.

菌株在以葡萄糖为碳源,添加番茄汁(3.6 mL /L)、芝麻油(1.6 mL/L)、Na2CO3(6 g/L)和磷酸缓冲盐的液体培养基中28 ℃振荡培养5 d,每mL培养液细胞生物量湿重达到65.57 mg,类胡萝卜素产量达到9.896 μg,菌体每g湿重细胞胡萝卜素含量为150.9 μg。

The result showed that the changes of stoma density ranged from 91~173 unitmm^(-2). Guard cell length ranged from 25.8~32.4 μm. Guard cell width ranged from 18.4~24.2μm. Guard cell width in sports variety was longer than in original variety.

结果表明:叶片气孔密度的变化范围在91~173个/平方公厘,保卫细胞长度变化在25.8~32.4μm之间;保卫细胞宽度变化在18.4~24.2μm之间,芽变品种的保卫细胞的宽度有变长的趋势。

We examined the effects of Gyp on human leukemia HL-60 and mouse WEHI-3 cells. Therefore, the results from flow cytomertic analysis indicated that Gyp arrest cell cycle in G0/G1 and induced apoptosis in both examined cell lines. We also used DAPI stain and DNA gel electrophoresis to confirm Gyp induced apoptosis in both cell lines.

本实验探讨绞股蓝皂甘是否对於人类血癌细胞株(HL-60)和小鼠血癌细胞株(WEHI-3)诱发抗癌活性机转,藉由流式细胞仪分析结果发现绞股蓝皂甘能够让细胞周期停滞於G0/G1期,最后走向细胞凋亡,此结果可经由DNA电泳观察到DNA断裂情形得到证实。

DMBQ, ferulic acid and resorcin were effective chemical signal substances in the process of seed germination and haustorium formation of Cistanche deserticola. 4.The embryo is consist of 12 cells, the endosperm cell degraded so as to provide nutrition for the embryo development .As a result, germ tube differentiated from the embryo and came out through micropylar end, the small cell at the tip of the germ tube held the capacity of cleavage and the other cells grew big ,so the germ tube elongated .The anterior extremity of germ tube swelled to form haustorium under chemical substances treatment, the haustorium cell stained ,especially its portrait because of the lignification of the cells.

肉苁蓉种子的胚是由12个细胞紧密排列形成的圆球状胚,球形原胚分裂增殖的同时,胚乳细胞分解,为胚细胞的增殖提供营养,最终胚上分化出的芽管由珠孔端冒出,芽管前端细胞较小,具有旺盛的分裂能力,后部细胞体积增大,芽管逐渐伸长,在化学物质的诱导下,芽管由中部至前端细胞膨大分化出初生吸器,初生吸器呈帽子状,而这些细胞比其周围的细胞着色深,吸器纵向局部区域的番红染色加深,细胞高度木质化。

We examined cellular morphology under phase contrast microscope,cell proliferation using MTT assay,viable cell numbers by hemocytometer counting and cell apoptosis with flow cytometry.

当RA在高浓度(1μM、10μM、100μM)时产生明显抑制作用,细胞形态出现显著变化,48h较24h更明显。

Quiescent RASMCs were incubated respectively in 10% FBS DMEM as control group, 10% FBS DMEM supplemented with 0.1% dimethyl sulfide as vehicle group and 10% FBS DMEM supplemented with panaxynol(at concentration of 1,3,9×10-6 mol·L-1) as panaxynol groups. 1. Cell counting and double time. Cell viability was examined with trypan blue exclusion test and the cell number was counted at 0, 24, 48, 72h after the incubation using a hemocytometer.

实验中细胞分成如下几个处理组:①正常对照组:含10%FBS的培养液;②溶剂对照组:0.1%DMSO;③药物处理组:panaxynol 1、3、9×10-6 mol·L-1组。1、细胞计数及倍增时间测定:于接种并同步化后处理0、24、48、72h,用血细胞计数板计算活细胞数量,绘制生长曲线并计算细胞倍增时间。

Pike protein consists of S1 and S2 subunit. S1 is head and S2 is rod-shape part, intermolecular effort can bind each other. S1 can bind with host cell epimembranal acceptor. S2 can fix integral Spike protein on the cellular membrane and mediate cell-fusion with virus and host cell. There are significant antigen determinant of virus in Spike protein, genic heterogenesis can effect viral pathogenicity. SARS-CoV Spike protein, which has 23 pieces larvaceous glycosylation sit, consists of 1256 amino acid. Its N-terminals and C-terminals is corresponding conservative.

蛋白是I型膜糖蛋白,介导病毒与宿主细胞膜上的受体结合并与宿主细胞膜相融合。S蛋白由S1和S2两个亚基组成。S1形成球状部分,S2形成棒状部分,两者之间通过分子间的作用力相互结合。S1能与宿主细胞上的受体结合,S2可把整个S蛋白固定在细胞膜上,并介导病毒包膜与宿主细胞膜融合。S蛋白上有重要的病毒抗原决定簇,S基因的突变会影响病毒的致病性。

CD44 is a single strand glucoprotein family encoded by single gene on the memebrane surface with high heterology,which can mediate adhesion between cell and cell as well as cell and extracellular matrix.Its different splicing variants will result in the variety of its gene products.Especially,its splicing varant V6 (CD44v6) is condided to have oncogenicity.CD44v6 takes part in the genesis and development of tumor at some certain and is one of prognostic factors.So it will be the marker indicating tumor development.

CD44是由单一基因所编码的具有高度异质性的单链膜表面糖蛋白家族,介导细胞与细胞、细胞与细胞外基质之间的黏附作用,其拼接变异体的不同构成了其基因产物的多样性;尤其拼接变异体V6(CD44v6)被认为具有致瘤性;CD44v6在一定程度上参与了肿瘤的发生发展,是预后因子之一,并且可能作为肿瘤进展的标志物。

The rate of cervical intraepithelial neoplasia is very optimistic,the cell of non-typical grouing and the cell of heteromorphism hyperplasaia are not different from the cell of carcinoma in sita They blong to the same process of the disease which is a kind of continuous changing of disease.

宫颈癌前病变的比率相当可观,不典型增生和异型增生的细胞在本质上与原位癌的细胞无差异,是同一疾病过程,在上皮内连续的不同程度的病变,早发现、早治疗,有效降低子宫颈癌的发病率。

The rate of cervical intraepithelial neoplasia is very optimistic,the cell of non-typical grouing and the cell of heteromorphism hyperplasaia are not different from the cell of carcinoma in sita They blong to the same process of the disease which is a kind of continuous changing of disease. TO be discovered and treated early is an effeitive way of decreaisng the ratio of carcinoma of cervix uteri.

宫颈癌 病变的比率相当可观,不典型增生和异型增生的细胞在本质上与原位癌的细胞无差异,是同疾病过程,在上皮内连续的不同程度的病变,早发现、早治疗,有效降低子宫颈癌的发病率。

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