查询词典 cell-nests

Weightlessness simulated; Stomach neoplasms; Tumor cells, cultured; Cell size; Proliferating cell nuclear antigen; Cell cycler Apoptosis

失重模拟胃肿瘤肿瘤细胞，培养的细胞大小增殖细胞核抗原细胞周期脱噬作用

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后，通过与ONYX-015（E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒，美国ONYX生化制药公司研制）、wtAd5进行对比，利用病毒增殖实验和细胞生长抑制实验，验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用；利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异，揭示了腺病毒肿瘤靶向性的机制；利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株，直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

Methods: Introduce cytopathology experimental methods, human hepatic cancer cell line Bel-7402 co-cultured with Triptolide (10 g/mL, 5μg/mL and 0.25μg/mL), Using MTT method to test the effect of Triptolide on Bel-7402 cell proliferation, using TUNEL technique to test the effect of Triptolide on Bell-7402 cell apoptosis, immunohistochemistry staining method to test Bax and Bcl-2 protein expression.

采用细胞药理学实验方法，人肝癌细胞株Bel-7402与雷公藤甲素（10μg/mL、5μg/mL及0.25μg/mL）共培养，MTT法检测其对Bel-7402细胞增殖的影响，TUNEL技术检测其对Bel-7402细胞凋亡的影响，免疫组织化学染色法检测Bax及Bcl-2蛋白表达。

Results 2 ClAdo and 2 CldAdo had different cytotoxicities on the cells, while 2' dAdo had no cytotoxicity on the cells. 2 CldAdo blocked the cell growth at the phase of S and G2/M, while 2 ClAdo at the phase of G0/G1. 2 CldAdo mainly induced cell necrosis, while 2 ClAdo induced both cell necrosis and apoptosis. Down regulated Bcl 2 expression and up regulated p53 and Bax expression were associated with 2 ClAdo, while 2 CldAdo down regulated Bcl 2 expression and up regulated mildly p53 expression without any effect on Bax expression.

结果 2 ClAdo、2 CldAdo对所研究的细胞均有程度不等的毒性；2' dAdo对各细胞生长、增殖均无明显抑制（c=100 μmol/L即30 μg/mL内）；2 CldAdo明显阻断细胞于S、G2/M期，2 ClAdo阻断细胞于G0/G1期；2 CldAdo 致细胞坏死为主，2 ClAdo 既致细胞坏死又诱导凋亡；2 ClAdo 下调Bcl 2，上调P53、Bax蛋白表达；2 CldAdo小幅上调P53、小幅下调Bcl 2，不影响Bax蛋白表达。

They existed as dosage-effect and time-effect. All of these results proved that Sertoli cell was the first target cell, spermatogenic cell was the second one and mitochondrion was the target. Otherwise, we proved this compound can disturb the development and growth of sperms, influence the activity and amount of sperms and increase the deformation ratio.

结合组织病理和性激素及酶活性的改变，揭示该化合物睾丸毒性的靶细胞是支持细胞，其次是生精细胞，作用的靶点是细胞内的线粒体，此外还证明该化合物干扰精子的生长和发育，影响精子的活力和数目，增加畸形率，是雄性精子畸变的诱导剂。

Under nitrate-limited conditions the growth of Skeletonema costatum increased significantly due to its small cell size and lower demand for nitrogen, while the two dinoflagellate with large cell size increased slowly and resulted very low cell yields.

属于甲藻的微小原甲藻和塔玛亚历山大藻与属于硅藻的中肋骨条藻相比，具有较低的营养盐亲合力和比生长速率。

Disarrangement of regenerated hepatic cell nodus and liver cell cord,fatty degeneration in hepatic cell,thickening of spleen capsule and serious congestion of spleen parenchyma,atrophy of white pulp and shrinking of follicle and germinal center R50%NSG and RNSG could be seen that hepatic fibrosis,spleen capsule thichening and congesition of splenic parenchyma to a less extent.

脾内纤维严重增生。R50％NSG和RNSG光镜下见肝纤维化程度减轻，脾包膜变薄，脾实质轻度充血。

The major functions of the system include basic information management of cell lines, image management, document management, karyotype analysis, cell cryopreservation and anabiosis operation, drawing of cell growth curve, and etc. It was well established for its powerful functions and flexible operations.

其主要功能有：细胞系基本信息的管理、照片管理、文献管理、核型分析、细胞冻存与复苏操作、生长曲线绘制等，具有功能全面、操作简便、直观，实用等特点。

Each cell was mapped by the center point on the cell of transverse section with extended up and down for four pixel (nine-pixel lengths in total) and two pixels in width, and thereafter line convoluted dot map was obtained. Through Fourier transformation, the frequency distribution of power spectrum pattern from both horizontal and vertical direction was obtained, and the cell diameter in radius and tangential direction was known from the maximum value except 0 in the distribution.

把每个纤维细胞都用其中心的一个长9个像素点、宽2个像素点的线段点图表示，进行傅立叶变换，其能量光谱图在水平和垂直方向上的频率分布，除零值以外的最大值，即为细胞径向、弦向直径。

Fourier transformation image-processing technology was applied for determining the cross section fiber cell arrangement in hardwood. In this method, the disc-convoluted dot map from each cell radius with 10 pixels was transformed by Fourier transformation, and generated the angle distribution function in the power spectral pattern. The maximum value reflected the arrangement of the fiber cell.

利用傅立叶变换图象处理技术来测定阔叶材横切面纤维细胞排列，把表示每一个纤维细胞的半径为10个像素点的圈盘点图，进行傅立叶变换，再做能量光谱图的角度分布图，其最大值表示纤维细胞的排列。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。