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Routine detecting techniquefor cell immunity such as white blood count and differential, lymphocyte blastogenesis test has beenused, and combine with flow cell technology to detect the level of cell immunization after advancedimmunization at several important aspect, and tracking experiments to observe changes in the numberof the WBC and lymphocytes, lymphocyte proliferation, the dynamic changes of CD4~+ and CD8~+lymphocyte subsets andγ-IFN; and detected dynamic changes rules of IgG, IgM in humor andmucosa with indirect ELISA, then compare with ordinary immunization methods.

主要采用白细胞计数和分类计数、淋巴细胞转化试验等常规细胞免疫检测技术结合先进的流式细胞技术从细胞免疫的几个重要方面入手检测猪瘟超前免疫后细胞免疫的水平,并进行跟踪试验,观察其白细胞、淋巴细胞数目变化情况,淋巴细胞增殖情况,CD~(4+)和CD~(8+)淋巴细胞亚群及γ-IFN的动态变化规律;同时通过间接ELISA方法检测体液和黏膜中各IgG、IgM的动态变化规律,同时与普通免疫方法进行比较。

Results:Monoclonal antibodies IH3,5A12,7E5,7F9 showed specific respo nse to NIH3T3-erbB2 cell and SKBR3 cell,no response to NIH3T3 cell.1H3 also boun d specifically to the extracellular domains of P185 protein that expressed by E.

结果:单克隆抗体1H3、5A12、7E5、7F9可与NIH3T3-erbB2细胞、SKBR3细胞特异性结合,与NIH3T3细胞无反应;1H3与原核表达的c-erbB2胞外区蛋白有特异性反应,而与无关蛋白KDR无反应。

The most effective way to ensure that all design rules are met to keep all structures within a cell that are not connected to the adjacent cell at least half of a design rule away from the cell boundary.

满足设计规则最简单的方法,就是让色层离边界的距离超过设计规则中最小间距的一半以上。

Results The cell size appeared uniform,cell boundary was distinct,the cell recovery rate after frozen storing was above 90%.

出于对该突变细胞系的重视,我们不仅对其精心培养,同时进行了克隆,成功地获得了稳定的不死性的对病毒广谱敏感的克隆株,分别在汕头华茵和北京等三个实验室保存。

If all the flows getting into the system were constrained by the leaky-bucket parameters, the maximum cell delay and the buffer content bound were got when the capacity of the crosspoint was enough, and the maximum cell delay and the buffer capacity bound which can guarantee the maximum cell delay were also got when the content of the crosspoint was not enough.

在各数据流都由令牌桶参数约束的条件下,得到了交叉点缓存容量足够大情况下的最大信元时延和缓存临界容量大小,还得到了交叉点缓存容量不足够大情况下的最大信元时延以及保证信元具有时延上界的缓存临界容量大小。

Both cell proliferation and cell apoptosis are important in condyle development. And Bcl-2 gene family plays a crucial role in regulation of cartilage cell apoptosis.

软骨细胞的增殖和凋亡是髁突软骨发育中重要的生物学基础,而Bcl-2基因家族是软骨细胞凋亡调控网络中的关键因素。

It has several kinds of functions : It could accelerate blood coagulation; has chemotaxis to monocyte and neutrophil , could appeal to fibroblast and cartilage cell to move to the region of the injury, takes part in the signal transduction and activation in cell , and takes part in the growth and differentiation of cell and repair in trauma.

它的功能多样,能加速血液凝固,对单核细胞和中性粒细胞具有化学趋化作用,能吸引成纤维细胞和软骨细胞向损伤区域移动,参与细胞的信号传导与活化,细胞的生长及分化,创伤修复等一系列重要生理和病理过程。

The major products are exported (80%), including cell culture plates, bottles of cell culture, cell plate, and the needle-type vacuum filters, and the transfer of the centrifuge tube pumping, such as straw, as well as the excellent quality and highly competitive prices, the majority of customers were endorsed unanimously.

现产品主要出口国外(80%),其中,细胞培养板、细胞培养瓶、细胞培养皿、真空式与针头式过滤器,移液管与抽吸管以及离心管等产品以优良的品质和极具竞争力的价格,受到广大客户的一致认可。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在桑葚胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在桑葚胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

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