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K562 cell cultivated in suspension and adhesively cultivated with MSC were collected respectively and cell proliferation curves were drawn; the cell cycle was determined by flow cytometry; the effect of chemotherapy on cellular viability and apoptosis of...

对悬浮培养和与MSC黏附培养的K562细胞绘制细胞生长曲线;用流式细胞术测定细胞周期并观察化学药物对细胞存活率和凋亡的影响;用RT-PCR技术检测MDR1基因表达。

Due to its excellent water-solubility, it can permeate the epidermic cell and gland to be absorbed, improve the alimentation of skin from the internal to the external and promote the metabolism of cell. Because it has the effect to improve the alimentation of skin and promote energy and spring of skin' cell, there is a high value to develop the project.

因其优良的水溶性,可透过表皮细胞和腺体而被吸收,由内而外改善肌肤营养,促进细胞的新陈代谢,具有改善肌肤营养、增强皮肤细胞活力和弹性的功效,因此该项目开发价值极高。

In the article a optical system was developed by the newest RC microscope with the schlieren method, which made the figure of appearance excellent colour and lustre and fine third dimension. The special cylindrical and cubic crystal growth cell was designed and the temperature-control system was developed to cooperate with the cell. Radial thermal gradient was formed in the cylindrical cell while transverse thermal gradient in the cubic cell, which was prepared for finding new effects.

在实验中首次采用最新式的浮雕相衬显微镜和纹影法光路相结合的方法,使得实时观察到的形貌图有良好的色泽和三维立体感;自行设计了柱型和方型两种型号的晶体生长室,研制了适合这两种晶体生长室的温度控制系统,在柱型晶体生长室中形成中心径向的温度梯度,在方型晶体生长室中形成横向的温度梯度,从而为发现新效应创造条件。

Ultrastructurally, the nuclear membranes of lymphocyte and reticular cell were broken; the rough-surfaced endoplasmic of lymphocyte, reticular cell and plasma cell were swelling in immune organs; the crista of mitochondrion was broken in plasma cell of spleen.

胸腺、法氏囊中淋巴细胞和网状细胞的核膜局部断裂,粗面内质网肿胀;脾脏中浆细胞线粒体嵴断裂,粗面内质网肿胀。

Result vitamin A and 4HPR fall to Hela cell collect form have apparent inhibition, can restrain Hela cell to be opposite the action causing tumor of naked rat (the curb tumour action of P.01); vitamin A issues visible cell solid by force to shrink at 4HPR(P.01); report lens, nuclear film is screwy, nuclear chromosome gathers agglomerate stand by the wither such as nuclear film to die DNA of cell of cellular feature; by degradation, a collection of illustrative plates of typical " of shape of " a flight of stairs appears in electrophoresis of agaragar candy gel.

结果维生素A和4HPR对Hela细胞集落形成有明显抑制功能,并可抑制Hela细胞对裸鼠的致瘤功能(P.01);维生素A的抑瘤功能强于4HPR(P.01);电镜下可见细胞固缩,核膜扭曲,核染色体聚集成块并靠近核膜等凋亡细胞特征;细胞DNA被降解,在琼脂糖凝胶电泳中呈现典型的&阶梯状&图谱。

His problem can be solved by adopting parameter configuring and TPCR model. The TPCR IP core is made up of the padded delay cell and re-synchronization cell. The padded delay cell specifies and restricts the interface timing of the IP core, making it possible for the SOC designers to estimate the margin of the interface timing at every design stage and making the IP core integrated in SOC seamlessly without the glued logic. The re-synchronization cell bridges different clock domains and asynchronous signals, ensuring that IP core carries out the transfer of the asynchronous signals.

PCR IP核模型由弹性延迟单元和再同步单元组成,其中,弹性延迟单元规范了IP核的接口时序约束,使得SOC集成者能够在设计的各个阶段,估算IP核的时序裕度,并能够在集成时无缝集成到SOC中而无需加入粘合逻辑;而再同步单元增加了IP核接口在不同时钟域和异步信号之间的桥接功能,保证IP核能够可靠地完成异步数据的传输。

This experiment selects material E14d SD the big mouse SVZ nerve stemcell as well as P7d the SD big mouse internal ear stem cell, does nothave the blood serum complete culture medium raise through D-MEM/F12to cause its massive multiplication, a part carries on Nestin, NF,CNP, GFAP, MAP-2, Myosin, the P27 immunity group, proved the raise thenerve stem cell has many to the split up ON potential; Another partdyes EGFP after the extension to carry on the guinea pig internal earto transplant, after two weeks fills the class to take the cochleafixed slice observation, discovered has the stem cell ball group inthe internal ear survival, proved the nerve stem cellheterotransplantation the possibility and for transplants the stemcell migration differentiation repair hair cell damage the thoroughresearch accumulation experiment experience.

本实验取材E14d SD大鼠SVZ神经干细胞以及P7d SD大鼠内耳干细胞,通过D-MEM/F12无血清完全培养基培养使其大量增殖,一部分进行Nestin、NF、CNP、GFAP、MAP-2、Myosin、P27免疫组化,证明培养的神经干细胞具有多向分化的潜能;另一部分通过转染EGFP后进行豚鼠内耳移植,两周后灌流取耳蜗固定切片观察,发现有干细胞球团在内耳存活,证明神经干细胞异体移植的可能性并为移植干细胞迁移分化修复毛细胞损伤的深入研究积累实验经验。

Transfer cell A specialized type of plant cell in which the cell wall forms protuberances into the cell, thus increasing the surface area of the wall and plasma membrane.

传递细胞:植物的一种特化细胞,其细胞壁形成突起深入细胞内部,因此增大了细胞壁和细胞膜的面积。

There are some little cells with diameter 4.5~8.3 μm in molecular layer. Purkinje cell is like a pear in Purkinje cell layer, it's base width is 17.8~25.6 μm, it's height is 18-30 μm. There are lots of rough endoplasmic reticulum, free ribosome, mitochondria, golgi body, lysosome, etc. in Purkinje cell. Granular cell ratio of nucleus to cytoplasm is high in granular layer.

分子层含少量小细胞,细胞大小在4.5~8.3μm; Purkinje细胞层细胞体呈梨状,底宽在17.8~25.6μm之间,高约为18~30μm,细胞中含有丰富的粗面内质网、游离核糖体、线粒体、高尔基体、溶酶体等;颗粒层的颗粒细胞细胞核大,细胞质少。

We used R1 cell line, primary established by Nagy in Canada, as ES cell to establish culture environment for ES cell, and successfully subculture R1 cell with LIF and gelatinized dishes and observed its growth characteristic.

同时,也初步建立包括129小鼠基因组文库筛选获得基因组DNA片段、基因组DNA结构分析、基因打靶载体构建、ES细胞体外培养、电穿孔基因转移、重组事件的药物选择和分子鉴定在内的ES细胞基因打靶技术。

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