查询词典 cell-nests

Methods Human prostate cancer PC-3 cell, breast cancer ER-75-30 cell and lung cancer A549 cell were cultured in RPMI medium 1640.Light microscope,transmissional electron microscope, flow cytometer and immunohistochemical method were used to observe apoptosis morphological changes of three kinds of tumor cell lines, time and dose effect relationship and to detect expression of bcl-2 and bax.

方法人前列腺癌 PC-3细胞株、乳腺癌 ER-75-30细胞株和非小细胞肺癌 A549细胞株经培养后，采用光镜、电镜、流式细胞仪和免疫组化方法研究 188Re诱导 3种肿瘤细胞凋亡的形态学变化、时间剂量效应关系、细胞周期依赖性以及凋亡相关基因 bcl-2和 bax在其中的表达情况。

In this thesis, we find that dlg is indispensible in the establishment of anterior-posterior and dorsal-ventral polarity of drosophila oocyte. Removal of Dlg function from the posterior follicle cells using the FLP/FRT system leads to disruption of oocyte skeleton reconstruction that is elicited by the failure of those posterior cells to differentiate normally in mid-oogenesis. We demonstrate that abnormity of Notch, JAK-STAT and EGFR signal pathway in dlg mutants contributes to this aberrant differentiation. dlg null mutant also blocks the normal differentiation of two groups of anterior follicle cell-stretched cell and centripetal cell, but not border cell, with a lower penetrance. However unlike the result in posterior follicle cells, Notch and JAK-STAT signaling are both undisrupted in all mutant anterior follicle cells, implying other fate determinants may be involved.

我们的研究发现，后端滤泡细胞中的Dlg在果蝇卵子发生中期卵母细胞前后轴和背腹轴建立过程中也是必须的，PFC中dlg完全缺失型突变引起PFC的分化异常，导致卵子发生中期卵母细胞骨架重组异常，Stau、Grk等极性决定蛋白定位错误。dlg突变阻碍了Notch、JAK-STAT、EGFR等调节PFC分化的信号通路的激活。dlg突变的PFC也没有获得前端滤泡细胞命运。dlg突变不影响前端滤泡细胞群中边界细胞的分化，但是在一定程度上影响伸展细胞和向心细胞的分化，并且这种影响不依赖于前端滤泡细胞Notch或JAK-STAT信号激活的异常。

A 1: Uninduced supernant of cell lysate by sonication; 2: Induced supernant of cell lysate by sonication; 3: Uninduced precipitate of cell lysate by sonication; 4: Induced inclusion body of cell lysate by sonication; 5: Purified ULBP4 protein; M: Low molecular marker proteins.

2.3 体外NKG2D结合实验和细胞因子分泌实验间接ELISA检测结果显示：原核表达复性后的ULBP4能与人NKG2D结合，而带His标签的无关蛋白则不能与之结合，说明复性后的ULBP4仍能维持正确的天然构像。

Results Using different concentrations of 5fluorouracil to deal with A549 cells, with the increase in drug concentration and treatment time,cell survival rate decreased significantly;and low concentration of 5fluorouracil induced the cell spread and increased vacuolization within the cytoplasm, acridine orange staining showed an increase in acidic vesicles,high concentration made the cell round and cell apoptosis.

结果不同浓度的5氟尿嘧啶处理A549细胞，随着药物浓度的增加和处理时间的延长，细胞存活率明显下降；且低浓度使细胞呈铺展状态，细胞质内空泡增多，吖啶橙染色显示酸性膜泡增多，高浓度使细胞变圆，出现凋亡状态。

The experiment showed:CQP can reduce lipid peroxid a injury of myocardial cell membrane,can inhibit PLA2 activity,CQP may improv e the cell membrane fluidity,may prevent the damage of cell membrane,reduce the impairment of myocardial cell.

而CQP可减轻心肌细胞膜的脂质过氧化损伤，抑制PLA 2活性，改善细胞膜流动性，保护心肌细胞膜，减轻心肌细胞损伤。

RESULTS:Our results indicate that the chemokine CCL5 can induce migration in humanbreast carcinoma cell line MCF-7 in vitro and suggest a potential role for Ezrin in theprocesses of breast cancer cell migration,invasion and possibly metastasis responsed toCCL5.We demonstrate that Ezrin siRNA specifically inhibits Ezrin expression of MCF-7cells and deregulates several functions such as cell motility and cell invasion,indicatingthat Ezrin plays a key role in the development of human breast cancer cells.It\'s suggestedthat Ezrin may act as a new anti-invasion therapeutic target for human breast cancer.

趋化因子CCL5促进人乳腺癌细胞MCF-7的趋化与侵袭，Ezrin蛋白的激活在此过程中起着重要的作用；成功构建Ezrin特异性siRNA表达载体，可用于探讨乳腺癌的侵袭转移机制，并为有效地抑制肿瘤的转移提供新的生物治疗靶点。

RESULTS The positive rate of P-gp170expression in cervi-cal cancer pre-radiotherapy was58%（22/38）.Among them,the positive expression rates were10/18（56%）,6/10,4/6and2/4in keratinizing,nonkeratinizing squamous-cell carci-noma,adenocarcinoma and adeno-squamous-cell carcinoma respectively.The positive rate of post-radiotherapy was90%（34/38）and among them,the positive expression rates were16/18（89%）,8/10,6/6and4/4in keratinizing,nonkera-tinizing squamous-cell carcinoma,adenocarcinoma and adeno-squamous-cell carcinoma respectively.

结果 宫颈癌组织P-gp170阳性表达在放疗前为58%（22/38），其中角化型、非角化型鳞癌、腺癌及腺鳞癌分别为10/18（56%），6/10，4/6，2/4例；放疗后其P-gp170阳性表达为90%（34/38），其中角化型、非角化型鳞癌、腺癌及腺鳞癌分别为16/18（89%），8/10，6/6，4/4例，P-gp170表达阳性强度较放疗前亦呈增高趋势。

The surface state and electrochemistry properly platinum-plated on open-cell foamed titanium electrode was investigated and he compared with IrTi anode and platinum-plated titanium toil cathode. The results show that open-cell foamed titanium can uniformly coat with excellent bonding strength platinum-plated, thickness coating 2 μm. The cathode release hydrogen voltage of platinum-plated on open-cell loamed titanium electrode is fall. The anode release oxygen voltage of platinum-plated on open-cell foamed titanium electrode is higher than Irli anode in high current.

采用电镀技术在多孔钛表面沉积了均匀的铂金属层，分析了多孔钛镀铂后的表面状态及电化学性能，并与IrTi涂层阳极及镀铂钛网阴极进行比较，结果显示：多孔钛板开孔孔隙表面可均匀沉积结合力良好的铂层，镀层厚度为2μm；镀铂多孔钛阴极析氢电位比镀铂钛网高，且在大电流下，阳极析氧电位比IrTi涂层阳极高。

Compared by middle-stage luteal cell, luteinized granulosa cells responded an increasion in PCNA even under hypoxic condition, which spoke for an important role of hypoxia in controlling the performance of cell proliferation. Similarity was proved between luteal cell and luteinized granulosa cells when it came to the performance of cell proliferation under a condition of hypoxia.

然而，意外地发现缺氧条件下黄体化粒性细胞之PCNA蛋白质表现量反而增加，此结果与中期黄体细胞之结果恰好相反，显示此时之细胞分裂旺盛而快速增生，与黄体发育初期之细胞生理相似，且缺氧条件可能扮演著重要的调控角色。

In Chapter Two, some methods such as electron microscope, DNA ending label and MTT measurement were used to studied the changes of apoptosis, necroses and cell vigour of leukaemia K562 cell trunk and HL-60 cell trunk irradiated with millimeter wave. Different effects of millimeter wave to leukaemia cell trunks and human marrow cells were observed clearly. Human marrow cells were not so sensitive to millimeter wave radiation as leukaemia cells.

第二章，利用电镜技术、DNA末端标记、MTT活力测定等方法，研究了毫米波对红白血病K562细胞株、人类急性早幼粒白血病细胞株HL-60及正常人骨髓单核细胞的细胞超微结构、细胞凋亡、细胞活力的影响，结果发现毫米波辐射对正常人骨髓细胞及白血病细胞的影响明显不同，正常人骨髓细胞所受影响较小，特别是功率密度小的毫米波对其无影响；而白血病细胞受辐射后发生显著的凋亡或坏死，细胞活力显著下降，该效应具有明显的功率窗和时间窗。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。