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He CD4〓 T-cell viability determined by the trypan blue test was over 90%. PBMCs from the recipients, after inactivated, were added in 96-well culture plates with donor CD4〓 T-cell cocultured at 37℃ for 24 hours, TJU103 and CTLA4-Ig were given for additional 48 hours coculture. The CD4〓 T-cell was collected and washed as anergic CD4〓 T-cell.

JU103联合CTLA4-Ig应用对供者T细胞增殖活性的影响根据TJU103有剂量限制性特点，选择TJU103最大抑制效应浓度50μg/ml，与不同浓度CTLA4-Ig（1μg/ml、10μg/ml、50μg/ml、100μg/ml）联合，对供者T细胞的增殖抑制率分别为（59.5％、90.5％、93.4％、95.8％），TJU103（50μg/ml）与低浓度CTLA4-Ig（10μg/ml）联合，增殖抑制率在90％以上，已接近最大增殖抑制率，为二者联合应用的最佳组合，与单独应用组比较，有显著的统计学差异（p＜0.05）。

The reproductive development process of birch was described as follows: Male inflorescences extended from apical bud in early June. In later June, stamen primodium was differentiated in the bract of male inflorescence and the differentiation of bract was generated in female inflorescence. During July, anther layers and archesporial cell were differentiated in anther, then archesporial cell developed into mother microspore cell. Pistil primodium came into being at the same time. In early part of August the meiosis of mother microspore cell started. In later August, mono-nucleus microspore was formed. After September, both male inflorescence and female inflorescence were dormant.

白桦的生殖生长发育过程如下：6月初，雄花序从顶芽中生长出来。6月中下旬，雄花序苞片上分化出雄蕊原基，雌花序分化出苞片。7月，雄花花药分化出花药壁和孢原细胞，孢原细胞演化为小孢子母细胞，雌花序苞腋处分化出雌蕊原基；8月中上旬，小孢子母细胞减数分裂，8月下旬形成单核小孢子；9月后雄花序以单核小孢子状念越冬，雌花序以雌蕊原基状念越冬。

Results 1、The CD11b-positive microglia were dyed low brown with small bacilliform cell bodies,ramified morphology,thin and long protuberance in CON and NIC group;In LPS group,the CD11b-positive microglia were dyed chocolate brown with more big roundish cell bodies,thicker and shorter processes;Most CD11b-positive microglia were dyed brown with bacilliform cell bodies,ramified morphology and few cells were dyed deep brown with more big roundish cell bodies,thicker and shorter protuberance in NIC+LPS group.

结果 1、CON组与NIC组CD11b阳性小胶质细胞胞体小，呈杆状，突起细长，染成浅棕色：LPS组小鼠脑内CD11b阳性小胶质细胞胞体增大，呈圆形，突起粗而短，染成棕褐色；NIC+LPS组少量CD11b阳性小胶质细胞胞体增大，圆形，突起变粗变短，染成深棕色，多数呈杆状，突起少、细长，染成浅棕色。

At 4th week, the percentages of Clara cells over bronchiolar epithelial cells were similar between groups. At 24th week, the percentages of Clara cells were reduced in mice exposed to TCDD, NNK, CSC, or TCDD+CSC exposure, but not in mice exposed to TCDD+1 mg NNK. Thus, these findings suggested that TCDD, NNK, CSC, or TCDD+CSC exposure injuried Clara cells to lose CCSP protein in mice lung.

在A/J mice 4周实验组中，TCDD或NNK各实验组Clara cell的数目(26.6%-34.3%)与控制组(34.2%)比较，并没有明显的差异；反观A/J mice 24周实验组中，除了TCDD+NNK (1 mg)实验组外，其他各实验组Clara cell的数目与控制组(55.9%)比较明显下降11.7%-41.4%，显示暴露NNK、TCDD、CSC或TCDD+CSC皆会伤害Clara cell，暗示Clara cell对这些环境毒物比其他呼吸道上皮细胞敏感。

The secondary xylem of woody plants is a product of the vascular cambium,its formation is a complicated type of plant cell differentiation, including cambium initiation, cambial cell division, cell expansion, secondary wall deposition and programmed cell death.

木本植物的次生木质部是维管形成层的活动产物，其形成过程是植物细胞分化的一种复杂类型，包括维管形成层发生、形成层细胞分裂、分化、细胞扩展、次生壁形成和细胞程序化死亡等阶段。

Impact of PCL capsulation on the mesenchymal stem cell activity studied in vivo, one plate culture with complete culture medium for control group with and two encapsulated transplantation for experimental groups(complete culture medium or basic culture medium for cell suspended) installed,after package and transplantation,retrieving capsulated cells for cell count,MTT assay,CCK-8 assay Daily for 8days to determine cell activity,and analysis growth curve.

以完全培养液平皿法细胞培养为对照组，设2个实验组进行聚己内酯囊管化骨髓间充质干细胞移植细胞活性研究。两个实验组分别以完全培养液和基础培养液悬浮细胞后封装移植，囊管内细胞回收后以细胞计数、MTT法、CCK-8法测定细胞的活性，共8天，进行生长曲线分析。

Observation using transmission electron microscopy found the irreversible centripetal movement of chloroplasts in chlorotic plants, as a result occurred in three sequential events:(1) chloroplast positions changed from face position (with long axis of chloroplasts at the cell walls perpendicular to light ray) to profile position (with long axis chloroplasts near the cell walls parallel to light ray),(2) chloroplast shapes changed from long elliptical to circular,(3) chloroplasts were separated from the cell wall and moved to the center of the cell.

透射电镜观察发现失绿叶片中的叶绿体发生不可逆的向心运动，整个过程大致经历以下几个阶段：(1)叶绿体从叶绿体长轴垂直于光线转变至叶绿体长轴平行于光线；(2)叶绿体的形状从椭圆形转变为圆形；(3)叶绿体脱离细胞壁向细胞中心运动。

Methods: morphological change observation, live cell accounting and clone formation were used to investigate the inhibitory effects of Cirsium setosum MB on human chronic myloid leukemia K562 cell, hepatoma HepG2cell, cervical carcinoma Hela cell and gastric carcinoma BGC823 cell.

细胞形态观察、活细胞计数法和克隆形成率法研究小蓟水提液对人白血病细胞K562、肝癌细胞HepG2、宫颈癌细胞Hela、胃癌细胞BGC823生长的抑制作用。

Based on these previous work, in this research, in order to explore the the molecular mechanism of enhanced tumor cell immunogenicity treated by elemene and mytomycin C compositely, we used murine Hca-F ascitic hepatic carcinoma ( high lymphatic metastasis substrain of H22) as an experimental model to compare the immunoprotective effects on Hca-F cells as well as the mechanism of the effects caused by HSP70-peptide complex purified from untreated tumor cells, EC-TCV and BCG; We studied the mechanism of increased tumor cell membrane HSP expression treated with elemene by the techniques of RT-PCR and immunoelectron microscopy; In order to deeply elucidate the antitumor effects and the molecular mechanism of elemene in increasing tumor cell immunogenicity we analyzed and compared the effects of elemene and heat shock on the gene expressions of tumor cell using gene expression profile chip.

本论文在上述研究成果的基础上，选择小鼠肝癌H22的高淋巴道转移亚系Hca-F为模型，进一步比较了未处理肿瘤细胞来源、榄香烯复合瘤苗来源的和卡介苗来源的HSP70-肽复合物对Hca-F的免疫保护效应、效应机制，探讨榄香烯-丝裂霉素复合处理增强肿瘤细胞免疫原性的分子机制；以Hca-F和人肝癌细胞株HepG〓等为模型研究了榄香烯处理增强肿瘤细胞膜热休克蛋白表达的机制；以HepG〓为模型用表达谱基因芯片研究比较了榄香烯与热休克处理对肿瘤细胞基因表达的影响，从而进一步阐明榄香烯抗肿瘤作用及增强肿瘤细胞免疫原性的分子机制。

In contrast to conventional methods, a novel cell sheet-based technique has been proposed .This technique intend to achieve high cell density and function by layering confluent viable cell sheets without the use of acellular scaffolds that limit mass transport. Using thermo-sensitive cell culture surfaces, confluently cultured cells spontaneously detach as intact sheets simply by reducing the culture temperature.

与传统方法相比，有学者提出了一种新的以细胞层为基础的组织工程技术，这种技术作为一种新的选择，利用温敏性培养皿，经简单的温度变化，可以非损伤性的一起获得完整的培养细胞层及其所沉积的细胞外基质，从而避免了使用蛋白水解酶。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。