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Actinomycin D is the potent inhibitor of RNA synthesis, and is demonstrated to be able to induce Sf-9 cell, K562 cell, aleolar macroages cell CTLL-2 and Jurkat cell apoptosis.

放线菌素D是RNA合成抑制剂，具较强的诱导细胞凋亡的能力，实验证明放线菌素D可诱导Sf-9细胞，人白血病细胞株K562，肺泡巨噬细胞，CTLL-2和Jurkat细胞等凋亡。

K562 cell cultivated in suspension and adhesively cultivated with msc were collected respectively and cell proliferation curves were drawn; the cell cycle was determined by flow cytometry; the effect of chemotherapy on cellular viability and apoptosis of k562 cell was investigated,the mdr1 gene expression was determined by rt-pcr.

对悬浮培养和与msc黏附培养的k562细胞绘制细胞生长曲线；用流式细胞术测定细胞周期并观察化学药物对细胞存活率和凋亡的影响；用rt-pcr技术检测mdr1基因表达。

K562 cell cultivated in suspension and adhesively cultivated with MSC were collected respectively and cell proliferation curves were drawn; the cell cycle was determined by flow cytometry; the effect of chemotherapy on cellular viability and apoptosis of K562 cell was investigated, the MDR1 gene expression was determined by RT-PCR.

叶悬浮培养和与MSC黏附培养的K562细胞绘制细胞生长曲线；用流式细胞术测定细胞周期并观察化学药物对细胞存活率和凋亡的影响；用RT-PCR技术检测MDR1基因表达。

An object-oriented Petri Net will be built to model a flexible manufacturing cell. This cell includes a robot, three machines and a WIP buffer. The job enters the cell once the job volume is under capacity limit, therefore adynamic scheduling system is required to schedule operations for jobs in the cell. An algorithm will be developed for the scheduling system, which considers job's makespan as criteria.

在研究中，采用物件导向裴氏图模式，架构一个阶层式之弹性制造系统，其中包含机器群组主电脑物件、机器电脑物件以及机器手臂电脑物件，并规划系统的动态进出排程，以工作总完工时间为绩效评估指标来发展演算法，接著建立eM-Plant的模拟系统，以资料库建立之工件加工档，进行动态排程及模拟。

The structure of cell wall was not destroyed by the algaecide. So we could know: after being contacted with the algae, the algaecide could penetrate the cell wall and reacted with the protein directly, damaging the function of cell and killing the cell at last.Furthermore, we blended the isothiazolone with the high polymer, such as chitin, chitosan and poly, and prepared the slow-release drug films.

实验发现，异噻唑啉酮对棕囊藻的细胞形态结构破坏程度不大，表明了异噻唑啉酮对藻体的细胞壁、细胞膜具有很强的穿透作用，能够快速穿透细胞壁和细胞膜，与细胞内部的蛋白质作用，抑制细胞生理功能的正常发挥，导致藻体死亡。

As exposed to optics microscope and inverted microscope,compared to control,SW480 cells with Giemsa staining that treated with the H2RA(cimetidine、nizatidine) took on malignance declining as crimpled firstly,cellular bulk and heteromorphism dimimished,the amount of rounded cell manifolded,nucleolus bulk lessened,dyeing thin,the amount of nucleolus decreased,partial cell organs manifest side-gathering phenomenon on chromatin,and the link between SW480 cell is loosened , subsequently partial rounded cell is floated.When the H2RA(cimetidine 40μM、nizatidine 20μM) against SW480 cells after 3 day,SW480 cells change roundly,nucleolus smashed,shrinking nucleolus came into being,and part of those not adhibited cell wall.

在光镜和倒置相差显微镜下，与对照组相比，经姬姆萨染色观察，西咪替丁、尼扎替丁处理的SW480细胞，先发生皱缩，体积变小，异形性减少，细胞变圆数目增多，细胞核变小，染色变淡，核仁数量减少，部分可见染色质边集现象；细胞间连接疏松，随后部分变圆细胞浮起。40μM西咪替丁及20μM尼扎替丁连续处理3天后，SW480细胞全部变圆，细胞核碎裂，固缩微核形成，部分不再贴壁。

Factors involved in the expression of IFN -αwere studied. These included multiplicity of infection, cell inoculation concertration and cell growth phase. The results show that lower mulitiplicity of infection (M01.4PFU/cell) is beneficial to IFN-α production.The optimal cell inoculation concentration was 〓 cells/flask. The high efficient replication of BmNPV-IFN-α and expression were also due to the proper inoculation time of BmNPV-IFN-α, which was at 48-60 house after cell passage.

研究结果表明，重组病毒感染复数的改变对α-干扰素的产量的影响是时限性的，在一定低感染复数时（Mo1.4PFU），α-干扰素的产量较高；对于重组病毒的复制，最适的细胞接种密度为3.6～7.0×〓细胞/瓶；在细胞指数生长前期（48～60小时）接种重组病毒，对于获得较高的重组病毒效价和α-干扰素的产量都是有利的。

Adding ouiduet epithelial cell or granulose cell to CR1aa or TCM-199 co-culture, the cleavage rate, 4-6 cell developmental rate and 8-16 cell developmental aresignificantly increased (P＜0.05). We can conclude that: CR1aa and TCM-199 containing 5％FBS were the samewith somatic cell cloning in culture in vitro in Yanbian Yellow Cattle.

使用SPSS14.0软件对结果进行方差分析并多重比较后，综合考虑实验结果和方便操作时间与过程，得出以下结论：含5％FBS的CR1aa和TCM-199培养液适用于延边黄牛体细胞克隆胚胎的体外培养。

Penetrating view about scholar , the article inherited the view of many scholars, and increased some of my own opinions, such as point out short information of cell phone concrete handle course " suggestion leader " function strengthen greatly , from " the media is the message " and " media "--The people one extension "watch short information of cell phone the the developments one rationality,"last " weaken reality of function "; In addition, the article has offered the objective standards of how to judge the short information of cell phone in order to be judged of the short information of cell phone through understanding communication of the short information of cell phone, thus know and have certain reference meanings to some mistakes which clarify people.

文章继承了有关学者、专家的精辟观点，并增加了自己的一些见解，如指出了手机短信具体操纵过程中&意见领袖&作用大大加强、从&媒介即讯息&和&媒介——人的延伸&看手机短信发展的合理性、&把关人&作用弱化等现实情况；另外，文章通过对手机短信媒体特点的分析，对澄清人们的一些错误认识也有一定的借鉴意义。

The Institute for Stem Cell Research is primarily dedicated to research and education in stem cell including developmental immunology, embryonic patterning, developmental epigenetics, cardiovascular stem cell, embryo stem cell neurogenesis, developmental haematopoiesis, embryo stem cell biology, genome engineering, bioinformatics, early embryo development, etc.

爱丁堡大学干细胞研究所主要致力于干细胞的研究和教学，其学科领域包括发育免疫学，胚胎模式，发育遗传学，心脏血管干细胞，胚胎干细胞神经发生，发育造血作用，胚胎干细胞生物学，基因组工程，生物信息学，早期胚胎发育等。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。