查询词典 cell-free

Methods MTT assay was used to determine the proliferation inhibition by cantharidin to A549 cell. FACS can were applied to detect the change of cell cycle.

采用MTT法检测斑蝥素对A549细胞增殖的抑制作用；流式细胞仪分析A549细胞周期及斑蝥素对细胞周期的影响。

Results Cantharidin inhibited the proliferation of A549 cell, and the mechanism of inhibitory action is to make A549 cell cycle hindered at G2/M.

结果 斑蝥素对A549细胞的增殖有抑制作用；其抑制作用机制为使A549细胞周期出现明显的G2/M期阻滞。

Besides, the series of synthetic compounds were tested for their capability to suppress growth of human hepatocellular carcinoma cell line Hep 3B and Hep G2, and myeloid leukemia cell line HL-60. The results showed compound 20, N-(2-amino- 3-nitrophenyl)cantharidinimide, exhibited more potent than others, but it was still less effective than cantharidin.

此外也利用斑蝥胺的衍生物对人类肝癌细胞Hep 3B和Hep G2，以及血癌细胞HL-60的细胞毒性进行测试，结果显示化合物20 [N-(2-amino-3-nitrophenyl)cantharidinimide]有较好的抑制作用，但是效果仍未比斑蝥素好。

In one kind of viral life cycle, the virus first binds to the cell's surface, then penetrates the cell and sheds its capsid.

在某种病毒的生命史中，病毒先是附着于细胞的表面，然后穿透细胞并蜕下其病毒外壳。

The losing of balance of cell proliferation and cell death is closely related to the carcinogenesis.

肿瘤的发生与细胞增殖和死亡之间的比例失衡密切相关。

Methods The peripheral blood lymphocytes,sera of 55 patients with rheumatic carditis and a specific antigen from group A streptococcal cell membrane were prepared and used as stimulants to test the heart tissue cell procoagulant activity in culture.

用风湿性心脏炎患者的外周血淋巴细胞、血清及A组乙型溶血链球菌膜抗原作为刺激物，测定体外培养的人胚心肌细胞促凝血活性。

HPCA was measured by using a one-stage plasma recalcification clotting time.Results The peripheral blood lymphocytes from rheumatic carditis patients can stimulate the procoagulant activity of human heart tissue cell in vitro.But the sera of rheumatic carditis patients and group A streptococcal cell membrane can not increase HPCA.

结果 患者的外周血淋巴细胞能使HPCA明显升高；静止期风心病、风湿性关节炎、其他疾病对照组及健康对照组患者的外周血淋巴细胞均无此特性，迁延活动型风湿性心脏炎患者的血清及A组乙型溶血链球菌膜抗原对HPCA也无直接影响。

The effect of carotenoid on the bcl-2 gene expression of breast cancer cell lines MCF-7 cell was determined by the method of RT-PCR.

采用RT-PCR方法检测类胡萝卜素对MCF-7细胞株的Bcl-2基因表达的影响，均呈不同程度的下调。

Methods The target DNA sequence of yrdC was obtained from human spleen tissue by using RT-PCR,construct high titer recombinant adenovirus Ad-yrdC by using AdEasy adenovirus carrier system,detect yrdC protein expression by using Western blot and immunohistochemical method,set up control group and transfected Ad-Null group,and study the effects Ad-yrdC on BGC-823 cell line by drawing cell growth curve and MTT chromatometry.

方法用RT-PCR方法获取人脾脏组织yrdC基因序列，采用AdEasy腺病毒载体系统构建携带yrdC基因的高滴度重组腺病毒Ad-yrdC，将其转染胃癌BGC-823细胞，应用Western blot和免疫组织化学法检测yrdC蛋白表达，设对照组和转染Ad-Null组，并分别绘制细胞生长曲线和MTT比色法研究Ad-yrdC对BGC-823细胞的影响。

Using this method, we observed the metabolic condition of cartilage cell and knew the effect of the serum with the various types and different concentration to the growth of cartilage cell.

利用体外细胞培养的方法进行生物学、医学领域的科学研究工作近年来在世界上甚为广泛，以软骨细胞培养的方法对软骨和骨关节疾病进行的研究也日趋深人，并已发展到分子水平。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。