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Methods HPDLFs were primary cultured from tissue explants, and the cells of the 5th to 8th passages were used after immunohistochemical identification of keratin and vimentin expressions. The cells were divided into 5 groups and treated with TP at 1, 0.5, 0.25, 0.125, and 0.0625 mg/ml, respectively, with another group without TP treatment as the blank control group. Cell counting and MTT colorimetric assay were performed to assess the cell proliferation, and flow cytometry was employed to determine the DNA content of the HPDLFs.

采用组织块培养法培养原代人牙周膜成纤维细胞并传代,经免疫组化SABC法检测角蛋白和波形丝蛋白鉴定,取5~8代细胞用于实验;按茶多酚不同浓度分1mg/ml、0.5mg/ml、0.25mg/ml、0.125mg/ml、0.0625mg/ml组和空白对照组,采用细胞计数法、MTT法检测细胞增殖情况,流式细胞术检测细胞DNA含量。

Early embryonic cell has the developmental totipotency. Every embryonic blastomere cell can develop to an intact individual separately in a proper condition.

早期胚胎卵裂球细胞具有发育全能性,每个胚胎卵裂球细胞在适宜的条件下都能单独发育为一个完整的个体。

Of reconstructed oocytes with 2-cell blastomere and 0.7% of reconstructed oocytes with ES cell developed in vitro to blastocyst.

结果表明不同分化程度的细胞核移植后,重新编程的难易程度不同,分化程度越高则重新编程越难。

From the difference of cleavage rate, rate of 4~8-cell embryo and rate of embryo of more than 8-cell, a best kind of semen for getting early embryonic blastomere cells in the lab could be screened out.

通过比较不同类型精液(鲜精、附睾精液和冷冻—解冻精液)IVF的卵裂率、4~8-细胞胚胎率以及8-细胞以上胚胎率差异,筛选出适合本实验室获得早期胚胎细胞来源的精液。

One embryonic cell can be separated into many blastomere cells. As be the nuclear donor of the embryonic cell nuclear transfer, it can breed the number of eminent species largely, increase the number of endangered animals, supply large number of supply same genotype animals, improve the efficiency of gene transfer and sex control and offer same genotype apparatus or organ for human transplant.

一个早期胚胎细胞可以分离多个卵裂球,以此作为核供体进行胚胎细胞核移植,可以大量扩繁优良品种的数量,增加濒危动物的数量,为科学研究提供大量遗传同质的动物,提高转基因和性别控制技术的效率,提供基因型相同的器官或组织供人类移植。

The colony of embryonic stem cell separated from blastula could gain five strains of embryonic stem cell and go down to posterity F7 in vitro.

从培养的囊胚中分离获得的类胚胎干细胞集落,经传代培养获得了5株胚胎干细胞株,在体外可传代培养到7代,冷冻保存复苏后,干细胞仍能存活、增殖。

GV oocytes with normal shape were not obtained in the group which was stored at 20℃ for 24 h and at 25℃ for 14 h; A blastula rates (64%) of all groups in average was gotten in culturing 2-cell in vitro, and there is no significant difference in temperature and time of storage. After transplantating some of the 2-cell embryos which were obtained from GV oocytes stored at 15'C for 8 h, normal infant mice could be obtained.

在20℃下保存24h和25℃下保存14h时,难以获得形态正常的GV期卵;体外受精获得的2细胞期胚经体外培养,总体上有64%的胚胎发育至扩张囊胚,未见有保存温度和保存时间的显著影响,且利用在15℃保存8h后的GV卵获得2细胞期胚的移植获得正常新生小鼠。

TS inhibition of T cell T-cell blood cells about 5% of the total to 10%.

抑制T细胞 TS细胞约占血液T细胞总数的5%~10%。

In the long time duration of studying on Bluetongue Virus of Hubei China strain 3, BTV-HbC3, We noted that BTV-HbC3 could be propagated well in HeLa cell line, a cell line derived from human cervical carcinoma, and later, we found it can even infect some other cancer cells.

本研究室在对蓝舌病毒中国湖北株-3(BTV-HbC_3)长期的研究过程中,注意到BTV-HbC_3可以在HeLa细胞,一种来自于人类宫颈癌的细胞,中良好地增殖,而后进一步发现其可以感染其他一些恶性肿瘤细胞。

Lecithin is needed by every cell in the body and is a key building block of cell membranes.

卵磷脂是需要的每一个细胞在人体中是一个关键的积木细胞膜的主要组成部分。

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推荐网络例句

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。