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There was significant difference in signal intensity between the cell concentration group and unlabelled cell group during 2 weeks. And significant difference occurred in the 5×105 SPIO-labeled cells per milliliter medium group and unlabelled cell group during 3 weeks.3. In kidneys of ischemia-reperfusion injury,the labeled BMSCs were demonstrated as signal intensity loss in renal medulla on T2*WI sequence in days 1,3,and5.The signal intensity loss was visualized up to 8 days after transplantation,and the signal intensity in the third day was the lowest. Histological analyses showed that most Prussian blue staining-positive cells were well correlated with the area where a signal intensity loss was observed in MRI.

在此细胞浓度的基础上,标记后细胞信号在T2*WI序列的降低在2w后失去统计学意义;而在细胞浓度为5×105/ml时,标记3w后信号在T2*WI序列的降低才失去统计学意义。3缺血再灌注肾损伤家兔细胞移植后第1、3、5天肾脏髓质区信号强度明显下降,至移植后第8天信号改变不明显,其中第3天信号改变最为明显;组织学分析见绝大多数普鲁士蓝染阳性标记细胞分布于肾小管周围,与MRI信号强度下降区域基本一致。

Apoptotic bodies were observed. Sulfone showed no effect on cell proliferation, cell cycle distribution or cell morphology.

而舒林酸砜化物对血管内皮细胞的增殖、细胞周期分布及凋亡无影响。

Inthe whole process, the oocytes, nurse cell and follicle cell morphology change. Inoocyte yolk formation and growth, the number of its nuclear trophoblast cell nucleoli,lamphrush chromosome with a strong synthetic material; oocytes also some syntheticmaterial; follicle cells in the yolk protein synthesis, provide access to exogenous yolkprotein.

在卵子发生的整个过程中,卵母细胞、滋养细胞及滤泡细胞形态均有明显变化;在卵母细胞生长及卵黄形成期,滋养细胞核内的多核仁现象、灯刷染色体与旺盛的物质合成有关;卵母细胞本身也合成一些物质;滤泡细胞参与卵黄蛋白的合成,并为外源性卵黄蛋白提供通道。

Then, on the basis of modeling the manufacturing cell with the O-O method, the information model of the manufacturing cell is described with XML language. It provides a method of information exchange to the optimization and configuration of manufacturing cell together with the development of application system.

其次,在制造单元信息模型的基础上,使用XML语言对制造单元信息模型进行了描述,为制造单元的优选配置以及应用系统的开发提供了信息交换的方法。

We compared the changes of T reg cell levels pretransplant and short courses posttransplant, the changes of T reg cell levels between the acute rejectors and the stables,and the changes of T reg cell levels between CsA group and FK506 group.

对比术前和术后近期T reg细胞水平的变化,移植肾功能稳定组和急性排斥组T reg细胞水平的变化,CsA组和FK506组T reg细胞水平的变化。

Methods Phase-contrast microscopy, cell proliferation assay and wound -closure were used to study the process of cell proliferation and wound healing in a model of human retinal pigment epithelial cell damage.

应用人RPE细胞损伤模型、细胞增殖曲线法,在倒置相差显微镜下观察人RPE细胞在各种条件作用下的损伤愈合与增殖过程。

CTAB was used to pyrolyse plant cell and reacted with the released cell nucleic acid to form nucleic acid-CTAB complex, the cell extract was then deproteined with chloroform-isoamylol, at last RNA was selectively precipitated by LiCl.

采用CTAB裂解植物材料,与细胞中的核酸形成核酸-CTAB复合体,然后用氯仿一异戊醇去除细胞裂解液中的蛋白,最后采用LiCl选择性沉淀其中的RNA。

CTAB was used to pyrolyse plant cell and reacted with the released cell nucleic acid to form nucleic acid-CTAB complex,the cell extract was then deproteined with chloroform-isoamylol,at last RNA was selectively precipitated by LiCl.

但是一般高等植物组织内都含有多糖类物质,这些物质的存在严重影响RNA的提取,如果去除不净,将影响反转录、扩增等下游操作。

To understand the infectivity by porcine endogenous retrovirus with porcine skin fibroblast cell in vitro and in vivo, porcine skin fibroblast cell established by our laboratory were co-cultured with neo/HEK293 cell for the infection of RERV in vitro, and were subcutaneously transplantated to SCID (severe combined immuno-deficiency) mice for the infection of PERV in vivo, laying the foundation for valuation of biologic safety of xenotrans-plantation. The event of neo/HEK293 cells infected by PERV occurred during co-culture of porcine skin fibroblast cells with neo/HEK293 cells, expanding the rang of the infection of porcine endogenous retrovirus. Afterpig cells transplantated subcutaneously in SCID mice, the microchimerism (78.57%) of pig cells occurred widel, and there was phenomena of integration of PERV provirus (85.71%) in several organs or tissues remote from the injected sites, indicating infection of PERV in SCID mice in vivo. yet, there is no evidence of active viral replication in analysis of PERV env RNA of these tissues or organs.

为了解猪皮肤成纤维细胞PERV在体外和体内的感染性,通过建立猪皮肤成纤维细胞系,将所建细胞系与人胚胎肾293细胞体外共培养,并移植于严重联合免疫缺陷鼠皮下进行猪皮肤成纤维细胞PERV的体外和体内感染性实验,结果表明,猪皮肤成纤维细胞与人胚胎肾细胞共培养过程中,猪内源性逆转录病霉感染人胚胎肾细胞,进一步证实和拓宽了猪细胞PERV感染人细胞的范畴;猪皮肤成纤维细胞移植SCID鼠皮下后,导致SCID鼠发生猪细胞微嵌合(78.57%)和PERV在体内感染(85.71%)并且波及远离移植部位的多种组织或器官,但是并未检测出SCID鼠组织中表达PERV env RNA。

A number of lines evidence indicate that calcium treatments maintain the structure and function of the cell walls, increase the fruit firmness, decrease the activity of the cell wall degration enzymes and ethylene production, maintain the integrity and stability of the membrane, activate some oxidases, induce phytolexin and regulate cell function as a signal mobecular.

钙的防腐机制在于对果蔬采后腐烂的控制,保持细胞壁的结构与功能,增强果实硬度,降低细胞壁降解酶的活性,抑制呼吸,降低乙烯生成量,保持细胞膜的完整性,激活体内的某些氧化酶,诱导合成植保素,作为信号分子,调节细胞功能。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。