查询词典 cell-free

The recombinant viruses, with the gene of reference RV strain VP2, field strain T114 VP6 and T73 G1-serotype VP7, respectively, co-infected Sf9 cell at a multiplicity of infection of 0.5. Harvested the supernatants of culture when the cells were lysed fully (about 7-10 days), or harvested cells before cell lysates (about 4-5 days after infection) and then treated the cells with lysing solution. The supernatants of and cell lysates were ultracentrifuged twice with 40% sucrose cushion at 93 000g, and the sediment products were harvested with TNC buffer. The purified VLPs samples were separated by SDS-PAGE, and then stained by Coomassie blue and silver nitrate and detected by Western blot to analyze the composition of VP2, VP6 and VP7 and their specificity. The morphologic structure of recombinant VLPs was detected by EM.

含标准株VP2、地方株T114 VP6和地方株T73 G1型VP7蛋白编码基因的重组病毒以0.5 MOI共感染Sf9细胞制备2／6／7病毒样颗粒，待细胞完全裂解后收获培养基上清或感染后5天细胞裂解前收获细胞并用裂解液裂解，培养基上清和细胞裂解液样品两次40％蔗糖垫93000g超速离心，沉淀样品经SDS-PAGE胶分离，用考马氏亮兰和硝酸银染色以及Western blot检测样品中VP2、VP6和VP7蛋白的组成以及特异性，电镜检测重组病毒样颗粒的形态结构。

Animal size;control mechanisms;cell number;cell division;cell death

动物体型；调控机制；细胞数量；细胞分裂；细胞死亡

Based on the results of information investigation, the paper briefly summarizes the research and application of cell engineering technology in agriculture: Chromosome engineering,protoplast culture,anther culture, cell culture, tissue culture and somatic hybridization. The worldwide development and progress in the application of cell engineering in plant breeding are reviewed.

根据文献研究结果，系统地综述了染色体工程技术、原生质体培养、花药培养、细胞培养与无性系筛选、组织培养与体系胞杂交等细胞工程技术，在国内外农作物育种上开发应用后所取得的新进展、新成果，以及这些新成果的产业化，对促进中国农业生产的发展所起的作用。

Barley Grass Fiber offers an excellent source of amino acids in the form of polypeptides that enable faster, more efficient assimilation into the blood to fuel the cell building, cell regeneration, energy production and cell metabolism in supporting the physical and mental developments.

它提供了一种能受血液快速高效吸收的肽状氨基酸，有效支援细胞制造，细胞再生，衍生和细胞新陈代谢，在儿童和青少年的身心发展上作出关键性的贡献。

Nutri-drink is packed with amino acids sourced from Barley grass in the form of polypeptides that enable faster, more efficient assimilation into the blood in the support for cell building, cell regeneration,energy production and cell metabolism, which helps boost the human physical and mental growth, especially necessary to the children.

大麦养生活力素富含源自大麦苗的活性肽形态氨基酸，此形态的氨基酸更易更快为人体血管所吸收，有效的辅助细胞的修造，繁殖，代谢，为人体尤其是儿童的身体心智发展作出贡献。嫩化皮肤，使您容光焕发

August 21, 2008 — Specific skin-odor profiles can be used to identify basal-cell carcinoma, researchers reported at the American Chemical Society 236th National Meeting and Exposition. These findings may eventually translate into novel and effective methods of diagnosing not only basal-cell carcinoma, but also squamous-cell cancer and melanoma.

August 21， 2008 —研究者在美国化学会第236届年会与展览中指出，特定的皮肤味道档案可以作为辨识基底细胞癌的工具，这些发现最终可以变为基底细胞癌和鳞状上皮细胞癌、黑色素瘤之新的有效诊断方式。

METHODS:Based on the model of human umbilical vein endothelial cell, this study adopted Rose Bengal Stain, cell ELISA, immunocyto-chemical techniques to investigate the effect of berberine on PMN-endothelium adhesion and the expression of cell adhesion molecules.

以小檗碱加IL-1、TNF处理人PMN或人脐静脉内皮细胞后，用蛋白染料染色法研究小檗碱对PMN与HUVEC粘附的作用，用细胞ELISA、APAAP法研究小檗碱对细胞表面粘附分子表达的影响。

The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2).Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR.MCP-1 protein expression were detected by ELISA.

采用过氧化氢建立离体培养的血管内皮细胞(ECV-304)氧化应激损伤模型，取健康大鼠每日灌服不同剂量的鲜姜有效部位(200，400，800 mg·kg-1)或阳性对照药洛伐他汀(40 mg·kg-1)，取含药血清作为受试药物，用孟加拉玫红染色法测定单核细胞与内皮细胞的黏附力；逆转录聚合酶链反应测定细胞内细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子(VCAM-1)以及单核细胞趋化蛋白(MCP-1)的mRNA表达水平；酶联免疫吸附法测定MCP-1蛋白表达水平。

Objective To know if MCF-7 breast cancer cell line has breast cancer stem cell,and change of stem cell pool in diverse incubation circumstance.

以及在不同的培养条件下，干细胞池的变化情况。

Genistein ; human breast cancer cell line MDA-MB-453; proliferation ; cell apoptosis ; cell cycle

三羟异黄酮；人乳腺癌细胞MDA-MB-453；增殖；细胞凋亡；细胞周期

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。