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Methods DCs were pre pared from peripherad blood mononuclear induce d with gra-nulocyt e／macrophage colony-stimulating factor and interleukin-4.Apoptosis of he patochlangioma cells were induced with γ-radiation.We design these experiment groups including(1)coculture of DCs and apoptotic cancer cells and T cells,(2)co culture of DCs and necrosis cancer cells and T cells,(3)coculture of DCs and cul tured cancer cell and T cells,(4)cocultu re of DCs and T cell,(5)cocuture of DCs and cultured cancer cell.

用粒-巨噬细胞集落刺激因子加白介素-4(IL-4)从人外周血分化、诱导DCs、γ-射线在体外诱导培养的人胆管癌细胞凋亡，将DCs、T淋巴细胞和凋亡胆管癌细胞共培养，同时设计不同类型肿瘤细胞(坏死胆管癌细胞及培养胆管癌细胞)作对照，7d后，分离、富集DCs、T 淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。

Coli. Transmission electron microscopy was used to evaluate the cell morphology of both the normal and the treated E. coli. The observation with TEM suggested that silver nanoparticles lead to the formation of "pits" in cell wall of the bacteria, and silver nanoparticles could enter into periplasm through the pits and destroyed the cell membrane.

采用透射电镜观察了经纳米银粒子处理过的大肠杆菌细胞形态变化过程，结果显示纳米银粒子先在细胞壁上产生小的孔洞，通过这些孔洞进入周质空间，导致细胞膜成分渗漏和破坏细胞膜，进而进入细胞内部。

We generated EG4-GFP cell lines expressing enhancing green fluorescence protein which still maintained typical characteristics of pluripotent stem cells. We microinjected green cells into perivitelline of 8-cell stage embryos as well as aggregated them to 8-cell stage embryos with zona pellucida removed, to investigate developmental fate of EG4-GFP cells during formation of chimeric blastocysts.

我们建立了稳定表达绿色荧光蛋白的EG细胞系（EG4-GFP），发现该细胞仍能保持野生型的一般生长特性；通过显微注射和体外聚集得到细胞与8-细胞受体胚胎共同发育形成的嵌合胚，发现转基因细胞参与了胚胎早期发育过程中的生长与分化，大部分细胞集中于内细胞团，也有细胞分化为滋胚层细胞。

Affect on the liver pathological state after XIEZHIPING treated: the golden hamster hepatic tissue morphous structure of normal control was normal, which had regular hepatic lobules and no lipid droplet;that of model control manifested diffuse heavy adipose degeneration and heavy ballooning degeneration with inflammatory cell infiltrate which mainly composed of lympholeukocyte;that of high dose group showed midrange ballooning degeneration with chronic inflammatory cell infiltrate which mainly composed of lympholeukocyte and plasmocyte;that of media dose group indicated slight focus ballooning degeneration with a small quantity normal hepatic cells in some locals;that of low dose group appeared midrange adipose degeneration with a few lympholeukocyte cell infiltrate in the parts of central veins and port area.

泻脂平治疗后对肝脏病理状况的影响：空白对照组金黄地鼠的肝组织形态结构正常，肝小叶规则，无脂滴发现；模型对照组肝脏弥漫性重度脂肪变性，发生重度气球样变，伴见以淋巴细胞为主的慢性炎性细胞浸润；泻脂平高剂量组肝细胞呈现中度气球样变，伴见以淋巴细胞、浆细胞为主的慢性炎性细胞浸润；中剂量组肝细胞轻度灶性气球样变，局部可见少量正常肝细胞；低剂量组肝细胞呈中度脂肪变性，可见中央静脉及汇管区些许淋巴细胞浸润。

Combination with the practice of domestic aluminium production industry, the 3—D current distribution, magnetic flux density, electro—magnetic force, melt flow velocity field of three diffrent cells, that is, 80KA Soderberg cell with vertical stub, 155KA prebake cell, 160KA prebake cell were computed and the related computer—simulated pictures were drawn by use of the softwares we made.

本文利用电流分布、磁场、电磁力场和流场的计算软件，结合我国生产实际计算了80KA上插自焙槽，155KA预焙槽，160KA预焙槽三种不同槽子的三维电流分布，槽内磁感应强度，熔体中电磁力场，熔体流速场，绘制了相应的计算机仿真图，为了增加计算软件的可靠性、用铁棒法测定了80KA上插自焙槽，155KA预焙槽的铝液流速，测定值与计算值符合较好。

Cell-based therapy is a promiseful option.This review will focus on the present status of cell-based therapy.Myoblast transfer therapy is hindered by minimal distribution of cells after injection,immune rejection,and poor cell survival.The drawback of bone marrow...

成肌细胞移植面临的主要限制是注射后细胞分布差，免疫排斥和细胞存活率低；骨髓干细胞移植需要解决横向分化的低效率问题；而肌源性干细胞看来能更有效地再生表达dystrophin的肌纤维。

Results bFGF 2.5ng/mL promoted ECV-304 cell migration. Cell pseudopodia was increased and cell skeleton was rearranged.

结果 bFGF 2.5ng/mL促进ECV-304细胞迁移，迁移细胞突起增多，细胞骨架发生重排。

As results, TEM showed characteristic ultrastructure morphology of apoptosis cell with chromatin marginal condense, nucleus pycnosis, mitochondrion vacuoles and cell shrinkage in IR injury group and no apoptosis cells in control group. Increased TUNEL-positive cell and typical DNA laddering were found and cells apoptotic rate were approximately 20% by flow cytometry assay in experimental groups.

值得探讨。本试验通过流式细胞仪、生物电镜、TUNEL法、RT-PCR以及DNA电泳等试验方法研究了带血供同种异体骨移植过程的细胞凋亡现象，并检测了Fas、FasL、Caspase-1、Caspase-3、Bcl-2等凋亡相关基因在IR损伤时的表达及变化情况，初步探讨了骨移植时IR损伤中细胞凋亡的发生机制及其在IR损伤中的生物学意义，希望为今后更深入的研究打下一定基础。

Under the phase-contract microscope, cell multi-l ayer was shown to be a refractile sheet, which means the capacities of biomateri al to attract cell and maintain the cell attachment.

相差显微镜下复层细胞表现为折光带，这反映生物材料引导细胞贴附并保持细胞贴附的能力。

RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.

结果：用诱导分化液作用后，未见小的、伴有突起的放射状形态的神经细胞；抗β Tubulin Ⅲ对经神经元胶质细胞诱导分化液作用后的人成肌细胞、增殖培养液培养后的各代人成肌细胞及仅含20 mL/L HS分化液分化形成的肌管细胞染色均为阳性； C2C12细胞β Tubulin Ⅲ抗体染色阴性；上述所有细胞抗Neurofilament Mr 68×103和抗GFAP染色均为阴性。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。