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Distribution of silkworm Bm N cell attachment to Cytodex 3 microcarrier was observed and the influence of cell inoculation concentration and microcarrier concentration on cell growth was studied.

选择微载体Cytodex3对家蚕BmN(从SilkwormBombyxmori获得的细胞系)细胞进行高密度培养,并获得成功。

Methods: A mouse model of head and neck squamous cell carcinoma was first established with 4-nitro-quinoline-1-oxide in drinking water, then the expressions of MAGEA1 and MAGEA3 protein were determined by immunohistochemical in the different developments of head and neck squamous cell carcinoma, central and adjacent (<5 mm,>5 mm area) tissue of head and neck squamous cell carcinoma (n=41), and normal mucosa tissues.

利用诱癌剂4-硝基喹啉-1-氧化物饮水法建立头颈部鳞癌小鼠模型,用免疫组化法检测MAGEA1、MAGEA3蛋白的在各组中的表达,包括头颈部鳞癌形成过程中的不同阶段、41例头颈部鳞癌中心区、癌旁<5 mm区、>5 mm区及正常黏膜组织。

Methods: WTBZ〗 Telomeras e activity was detected in 38 cases of NPC, 15 cases of PNC adjacent tissues and 19 cases of chronic nasopharyngitis as well as in NHE1 cell lines, two other ca ncer cell lines and three other normal control cell lines by TRAP PCR ELISA and TRAP PCR PAGE respectively.

分别采用TRAP PCR ELISA及TRAP PCR PAGE检测了38例鼻咽癌、15例癌旁组织、19例慢性鼻咽炎和鼻咽癌HNE1细胞株、其它2种癌细胞株及3种正常细胞的端粒酶表达。

The chronic action is stimulating beta-cell proliferation, induction of islet neogenesis, and inhibition of beta-cell apoptosis, thus promoting expansion of beta-cell mass, as observed in rodent diabetes and in cultured beta-cells.

其长期作用是刺激β细胞增生,诱导胰岛新生与抑制β细胞碉亡,因而促进β细胞体积增大,与啮齿类动物糖尿病模型及体外培养的β细胞所观察到的相一致。

Objective: To observe the effect of nerve growth factor on cell cycle in PC12 cells, and to establish a cell cycle model of neuronlike cell in vitro.

目的:观察神经生长因子诱导PC12细胞周期的变化,以建立体外神经元样细胞周期的研究模型。

In treatment group, there was no progressive necrosis in stasis zone, and at 24 h post injury, capillary dilation, cell edema and inflammatory infiltration were lessened significantly. In control group, 2 wounds had progressive necrosis (accounted for 10% of the total). Cell edema, inflammatory infiltration and capillary thrombosis were serious, Until 72 hr post injury, cell edema did not subside and necrosis of the dermis worsened. 2. Pathological assessment of the stasis zone tissue: As compared with that in control group, in treatment group the dermis structure in stasis zone was intact and the collagenous fiber bundle was normal.

结果:①创面细胞形态学改变:伤后8h毛细血管扩张、细胞水肿,炎性浸润最明显;治疗组中央淤滞区无进行性坏死,伤后24h后毛细血管扩张、细胞水肿、炎性浸润减轻;对照组2个创面呈进行性坏死(占10%),中央淤滞区细胞水肿、炎性浸润明显,毛细血管血栓形成,伤后72h水肿仍明显,真皮坏死加重;②淤滞区组织病理学评分:治疗组与对照组相比,治疗侧淤滞区的表皮结构相对完整、胶原纤维束相对正常,粒细胞浸润小于5个/400倍视野。

The solar cell industry rapidly develops with an annual average growth rate of 30% in order to cope with the energy crisis and the environment pollution·The next task is how to im- prove the conversion efficiency of the solar cell, increase the production scale and decrease the manufacturing cost, which can reduce the cost of electricity generated by solar energy and make it reach the same as that by the normal method·The variety of the solar cell and its manufacturing technique,the bottle neck of the indus...

为了应对能源危机和环境污染,太阳能电池产业以平均年增长率为30%的速度飞速发展。摆在人们面前的课题是如何进一步提高转换效率、扩大生产规模和降低制造成本,使太阳能发电的成本降低到与常规发电相当的水平。介绍了太阳能电池的种类、制造方法、产业瓶颈和发展方向。

OBJECTIVE: To investigate a simple easy method for isolation of cell nuclei, and to provide a great quantity of active cell nuclei for studying neuronal nucleus transplantation, cell isolation-fusion and nucleus composition.

目的:探索简单易行的分离细胞核的方法,为神经元细胞核移植、细胞拆合以及细胞核成分的研究提供大量完好有活性的细胞核。

To explore the functions of SPANXA1 in cancerous phenotypes CL1-5 cells were transfected with a SPANXA1 expressing vector and evaluated by cell proliferation, cell migration, Matrigel invasion and colony formation assays in vitro as well as mouse metastasis assays in vivo. The results indicated that the induction of SPANXA1 could reduce cell invasiveness. In the other hand, immunostaining showed that SPANXA1 was predominately located at the nucleoplasm.

经RT-PCR验证得知SPANXA1在CL1-0的表现量比CL1-5多100倍以上,於是我们将SPANXA1转染在CL1-5细胞株中,并测量活体外的细胞生长速度试验、细胞迁徙实验、Matrigel侵袭实验、癌细胞群落形成实验及小鼠活体内细胞转移能力,探讨SPANXA1对癌细胞性状的影响,结果发现增加SPANXA1会降低癌细胞的转移能力,在另一方面,我们利用免疫萤光染色法侦测出SPANXA1是存在於核质中,并以西方点墨法再次证实SPANXA1是存在於核质中。

Expression of Delta2 in HeLa and H1299 cells showed that only Delta2- expressing H1299 cell, which is a p53 null cell line, increased cell viability and eliminated the cleavage of PARP after UV exposure.

在HeLa细胞以及H1299细胞中大量表现Delta2可以发现,UV照射之后只有在p53缺失的H1299细胞中可以防止细胞因为Delta2分解而走入细胞凋亡。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。