查询词典 cell-free

The cell to cell contact could facilitate the transformation. It was found that the B. subtilis strain, cultured not only in MM medium, but also in LB medium, could be transformed by cell released or chemically extracted DNA by the method of agar plate transformation, although the LB medium was previously believed not to support this process in traditional transformation protocol.

结果表明，该菌在这两种培养基中培养时均可在对数生长期向培养液中主动分泌有生物活性的DNA，且细胞间接触有利於转化过程的进行，暗示在本实验条件下DNA供体细胞可能具有某种主动功能。

The invention can further improve the photoelectric conversion efficiency of the solar cell, makes full use of the functions of the upconversion material to convert up the wide infrared spectrums into the visible light and combine the thin film solar cell and the material with the upconversion function, so as to form a novel high efficient solar cell.

本发明能够进一步提高太阳电池的光电转换效率。充分利用上转换材料的功能，将宽谱域红外上转换为可见光，将薄膜太阳电池与该具有上转换功能的材料相结合，形成一种新型高效太阳电池。

The study on development and differentiation of cells in bamboo culm is very limited,especially on formation and development of cell wall.Cell wall is the main substance of bamboo cell structure.

与木材相比，竹材细胞壁的研究工作仍处于起步阶段，对竹茎杆细胞的发育过程，尤其是细胞壁形成和分化过程的研究远远不够。

Cell Delineation is responsible for defining the cell boundaries at the originating endpoint (e.g., ATM cell switch or ATM workstation) in order that the receiving endpoint can reassemble (i.e., identify and recover) all cells associated with a data payload that has been segmented (i.e., cut up into ATM cells).

信元定界负责定义始发端（例如 ATM 小区交换机或 ATM 工作站）的小区边界，这样接收端可以将那些与已经分段的（也就是切割成数个 ATM 小区）数据有效载荷相关的所有小区重新聚集。

METHOD: The extract of sago cycas leaves was added to K562 and HL-60 cell culture fluid respectively, Cell clusters, live-cells,dead cells were calculated and cell protein was detected. The results were compared with P. B. S. control group.

细胞培养液中分别加入铁树叶(实验组1和实验组2)及P.B.S(对照组1和对照组2)，观察集落细胞数、死活细胞计数及细胞蛋白质含量。

Weightlessness simulated; Stomach neoplasms; Tumor cells, cultured; Cell size; Proliferating cell nuclear antigen; Cell cycler Apoptosis

失重模拟胃肿瘤肿瘤细胞，培养的细胞大小增殖细胞核抗原细胞周期脱噬作用

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后，通过与ONYX-015（E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒，美国ONYX生化制药公司研制）、wtAd5进行对比，利用病毒增殖实验和细胞生长抑制实验，验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用；利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异，揭示了腺病毒肿瘤靶向性的机制；利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株，直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

Methods: Introduce cytopathology experimental methods, human hepatic cancer cell line Bel-7402 co-cultured with Triptolide (10 g/mL, 5μg/mL and 0.25μg/mL), Using MTT method to test the effect of Triptolide on Bel-7402 cell proliferation, using TUNEL technique to test the effect of Triptolide on Bell-7402 cell apoptosis, immunohistochemistry staining method to test Bax and Bcl-2 protein expression.

采用细胞药理学实验方法，人肝癌细胞株Bel-7402与雷公藤甲素（10μg/mL、5μg/mL及0.25μg/mL）共培养，MTT法检测其对Bel-7402细胞增殖的影响，TUNEL技术检测其对Bel-7402细胞凋亡的影响，免疫组织化学染色法检测Bax及Bcl-2蛋白表达。

Results 2 ClAdo and 2 CldAdo had different cytotoxicities on the cells, while 2' dAdo had no cytotoxicity on the cells. 2 CldAdo blocked the cell growth at the phase of S and G2/M, while 2 ClAdo at the phase of G0/G1. 2 CldAdo mainly induced cell necrosis, while 2 ClAdo induced both cell necrosis and apoptosis. Down regulated Bcl 2 expression and up regulated p53 and Bax expression were associated with 2 ClAdo, while 2 CldAdo down regulated Bcl 2 expression and up regulated mildly p53 expression without any effect on Bax expression.

结果 2 ClAdo、2 CldAdo对所研究的细胞均有程度不等的毒性；2' dAdo对各细胞生长、增殖均无明显抑制（c=100 μmol/L即30 μg/mL内）；2 CldAdo明显阻断细胞于S、G2/M期，2 ClAdo阻断细胞于G0/G1期；2 CldAdo 致细胞坏死为主，2 ClAdo 既致细胞坏死又诱导凋亡；2 ClAdo 下调Bcl 2，上调P53、Bax蛋白表达；2 CldAdo小幅上调P53、小幅下调Bcl 2，不影响Bax蛋白表达。

They existed as dosage-effect and time-effect. All of these results proved that Sertoli cell was the first target cell, spermatogenic cell was the second one and mitochondrion was the target. Otherwise, we proved this compound can disturb the development and growth of sperms, influence the activity and amount of sperms and increase the deformation ratio.

结合组织病理和性激素及酶活性的改变，揭示该化合物睾丸毒性的靶细胞是支持细胞，其次是生精细胞，作用的靶点是细胞内的线粒体，此外还证明该化合物干扰精子的生长和发育，影响精子的活力和数目，增加畸形率，是雄性精子畸变的诱导剂。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。