查询词典 cell-free

Cell proliferation was measured with cell number,cell metabolic activity (WST-1 cleavage),DNA synthesis and cell cycle assayed with flow cytometry.

进行新西兰白兔心脏心肌成纤维细胞的培养；测定细胞计数，细胞活性（WST－1分解）和DNA合成，并以流式细胞仪测定细胞周期等以表示细胞增殖。

Methods: Cardiac myofibroblasts derived from heart of New Zealand rabbit were cultured and identified. Cell proliferation was measured with cell number,cell metabolic activity (WST-1 cleavage),DNA synthesis and cell cycle assayed with flow cytometry.

进行新西兰白兔心脏心肌成纤维细胞的培养；测定细胞计数，细胞活性（WST－1分解）和DNA合成，并以流式细胞仪测定细胞周期等以表示细胞增殖。

In HepG2-Teton cell lines,the induced expression of RX8 inhibited cell growth and the formation of cell colones,and deduced S phase cell number and the de novo protein synthesis.

在HepG2-Teton细胞株中，诱导表达的RX8能明显地抑制细胞的生长和细胞克隆的形成，并使细胞周期中处于合成期的细胞数减少和新生蛋白的翻译量降低。

Methods With cell floating method, which is separating the cultured cell from coverslip and putting the cell in front of perfusion tubing, whole cell patch clamp technique was used to record hASIC1a currents evoked by low pH external solution.

使用全细胞膜片钳技术，将记录细胞与玻片分离，并使细胞位于输液管前，呈漂浮状态，以记录在低pH值外液中，HEK293细胞的酸敏感性1a型离子通道的电流。

Methods: After LNCaP and PC3 cell lines were affected by 10, 25, 50, 75, 100 μmol/L curcumine respectively, the cell activity was assayed with MTT method at 5, 12 and 24 hours respectively; Electronic microscopy and flow cytometry were adopted to observe the morphological changes and the cell cycle of LNCaP and PC3 cell lines at 24 hours.

分别用10、25、50、75、100μmol／L浓度的姜黄素对LNCaP及PC3前列腺癌细胞株进行体外干预，5、12、24 h后四氮甲唑蓝比色法检测细胞生长活性；24 h后透射电镜观察细胞超微结构变化，流式细胞仪测定细胞凋亡及细胞周期的变化；5 h后Western印迹法检测细胞内IκBa的表达。

The main characteristic of the meridional cell averaged over 110~ 140°E is as follows: The monsoon cell in East Asia replaces Ferrel cell in January and Hadley cell in July in the Northern Hemisphere. In addition, its decadal change is very obvious.

2110～140°E的平均经圈环流主要特点是：1月东亚季风环流圈代替了北半球的Ferrel环流圈，7月东亚季风环流圈代替了北半球的Hadley环流圈，而且其10年际变化也比较明显。

The results show that follicular cells originate from non-germ cells,and its whole development process can be divided into four stages:scattered cell stage,unilayer flat cell stage,multilayer flat cell stage and granule cell secretion stage.

结果表明，滤泡细胞起源于非生殖细胞，并且其整个发育过程可分为4个时期：零散细胞期、单层扁平细胞期、多层扁平细胞期和颗粒细胞分泌期。

Methods: The CD34+ cells and AC133+ cells were gathered by MiniMACS, then the cell factors were divided into 6 groups. Through the ex vivo comparison cultivation under different cell factor combination stimulation, expansion of the CD34+ and AC133+ selection cells was observed and the clone formation of the CD34+ and AC133+ cells was also observed by using Half-solid Methyl cellulose under different cell factor stimulation (in7d, 14d, 21d.). The rate of cell apoptosis was determined.

常规富集人骨髓CD34+及AC133+细胞，应用本研究组设计并已经证实的细胞因子组合方式，对人骨髓CD34+及AC133+富集细胞进行体外对照培养，分别观察CD34+和AC133+富集细胞的扩增情况；应用甲基纤维素半固体培养法，观察不同组别培养7、14、21d CD34+和AC133+富集细胞的集落形成情况及细胞凋亡率。

Methods CD34(superscript +) cells and AC133(superscript +) cells were gathered by MiniMACS, then the cell factors were divided into 6 groups. Through the ex vivo comparison cultivation under different cell factor combination stimulation, expansion of the CD34(superscript +) and AC133(superscript +) selection cells was observed and the clone formation of the CD34(superscript +) and AC133(superscript +) cells was also observed by using Half-solid Methyl cellulose under different cell factor stimulation (during 7d, 14d, 21d.). The rate of cell apoptosis was determined.

常规富集人骨髓CD34及AC133细胞，应用本研究组设计并已经证实的细胞因子组合方式，对人骨髓CD34及AC133富集细胞进行体外对照培养，分别观察CD34和AC133富集细胞的扩增情况；应用甲基纤维素半固体培养法，观察不同组别培养7、14、21d CD34和AC133富集细胞的集落形成情况及细胞凋亡率。

Results Basal cell adenocarcinoma of prostate was composed of poorly differentiated solid carcinoma nests with many cellular nuclear mitoses arranged like basal cell carcinoma. Necrosis was in the middle of the carcinoma nest. Local differentiation to squamous cell, transitional cell and gland and structure like adenoid cystic carcinoma were found.

结果：前列腺基底细胞腺癌为分化差的实体癌呈基底细胞癌样排列，核分裂象多，癌巢中央伴坏死，可见局灶性鳞状细胞、移行细胞或腺管分化，也可出现腺样囊性癌样结构。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。